{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T06:41:55Z","timestamp":1793515315964,"version":"4.5.0"},"posted":{"date-parts":[[2026,3,23]]},"group-title":"Microbiology","reference-count":44,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-11-LABX-0024"],"award-info":[{"award-number":["ANR-11-LABX-0024"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"crossref","award":["ANR-20-CE18-0013"],"award-info":[{"award-number":["ANR-20-CE18-0013"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Conseil R\u00e9gional d'Ile de France","award":["DIMOneHealth"],"award-info":[{"award-number":["DIMOneHealth"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,3,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Apicomplexan parasites such as\n                  <jats:italic>Toxoplasma<\/jats:italic>\n                  and\n                  <jats:italic>Plasmodium<\/jats:italic>\n                  spp. rely on the sequential secretion of parasite apical organelles, called micronemes and rhoptries, to invade host cells. The claudin-like apicomplexan microneme protein (CLAMP) is a conserved protein that plays an essential role during host cell invasion in\n                  <jats:italic>Toxoplasma<\/jats:italic>\n                  and\n                  <jats:italic>Plasmodium<\/jats:italic>\n                  zoites. Previous studies have shown that CLAMP is essential in\n                  <jats:italic>Plasmodium<\/jats:italic>\n                  merozoites for erythrocyte invasion and also in sporozoites for the invasion of the mosquito vector salivary glands and of mammalian host hepatocytes. In\n                  <jats:italic>Toxoplasma gondii<\/jats:italic>\n                  tachyzoites, CLAMP forms a complex with two other microneme proteins, the Secreted Protein with an Altered Thrombospondin Repeat (SPATR) and the CLAMP-Linked Invasion Protein (CLIP). Both SPATR and CLIP are also expressed in\n                  <jats:italic>Plasmodium<\/jats:italic>\n                  sporozoites, and downregulation of SPATR impacts sporozoite infectivity in\n                  <jats:italic>P. berghei<\/jats:italic>\n                  . In contrast, the role of CLIP in sporozoites remains unknown. To study the function of CLIP, we used a CRISPR-assisted conditional genome editing strategy based on the dimerisable Cre recombinase in the rodent malaria model parasite\n                  <jats:italic>P. berghei<\/jats:italic>\n                  . Deletion of\n                  <jats:italic>clip<\/jats:italic>\n                  in\n                  <jats:italic>P. berghei<\/jats:italic>\n                  blood stages impaired parasite growth and prevented erythrocyte invasion by merozoites. Upon deletion of\n                  <jats:italic>clip<\/jats:italic>\n                  gene in\n                  <jats:italic>P. berghei<\/jats:italic>\n                  transmission stages, sporozoite development in mosquitoes was not affected, but invasion of the mosquito salivary glands was dramatically reduced. In addition, CLIP-deficient sporozoites were impaired in cell traversal and productive invasion of mammalian hepatocytes, associated with a defect in gliding motility, recapitulating the phenotype of CLAMP-deficient parasites. Collectively, our data demonstrate that CLIP plays an essential role in host cell invasion by\n                  <jats:italic>P. berghei<\/jats:italic>\n                  merozoites and sporozoites, and support a conserved role of the CLAMP-CLIP-SPATR complex in invasive stages of apicomplexan parasites.\n                <\/jats:p>","DOI":"10.64898\/2026.03.22.713516","type":"posted-content","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T11:40:19Z","timestamp":1774266019000},"source":"Crossref","is-referenced-by-count":1,"title":["The CLAMP-Linked Invasion Protein (CLIP) plays an essential role in\n                  <i>Plasmodium berghei<\/i>\n                  zoites"],"prefix":"10.64898","author":[{"given":"Tanaya","family":"Unhale","sequence":"first","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, CNRS, Centre d\u2019Immunologie et des Maladies Infectieuses, CIMI-Paris, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samhita","family":"Das","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, CNRS, Centre d\u2019Immunologie et des Maladies Infectieuses, CIMI-Paris, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carine","family":"Marinach","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, CNRS, Centre d\u2019Immunologie et des Maladies Infectieuses, CIMI-Paris, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Briquet","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, CNRS, Centre d\u2019Immunologie et des Maladies Infectieuses, CIMI-Paris, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Fran\u00e7ois","family":"Franetich","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, CNRS, Centre d\u2019Immunologie et des Maladies Infectieuses, CIMI-Paris, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lien","family":"Boeykens","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Biochemistry and Infla-Med Centre of Excellence, University of Antwerp, Campus Drie Eiken, 2610 Wilrijk, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yann G.-J.","family":"Sterckx","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Biochemistry and Infla-Med Centre of Excellence, University of Antwerp, Campus Drie Eiken, 2610 Wilrijk, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0525-6940","authenticated-orcid":false,"given":"Olivier","family":"Silvie","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, CNRS, Centre d\u2019Immunologie et des Maladies Infectieuses, CIMI-Paris, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026032502500496000_2026.03.22.713516v1.1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14645"},{"key":"2026032502500496000_2026.03.22.713516v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80511-5"},{"key":"2026032502500496000_2026.03.22.713516v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.019"},{"key":"2026032502500496000_2026.03.22.713516v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.019"},{"key":"2026032502500496000_2026.03.22.713516v1.5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011261"},{"key":"2026032502500496000_2026.03.22.713516v1.6","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-87114-4"},{"key":"2026032502500496000_2026.03.22.713516v1.7","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.202000305"},{"key":"2026032502500496000_2026.03.22.713516v1.8","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M112.024505"},{"key":"2026032502500496000_2026.03.22.713516v1.9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005791"},{"key":"2026032502500496000_2026.03.22.713516v1.10","doi-asserted-by":"publisher","DOI":"10.15252\/EMBJ.2022113155"},{"key":"2026032502500496000_2026.03.22.713516v1.11","doi-asserted-by":"publisher","DOI":"10.1126\/science.aap7847"},{"key":"2026032502500496000_2026.03.22.713516v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.06.030"},{"key":"2026032502500496000_2026.03.22.713516v1.13","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14432"},{"key":"2026032502500496000_2026.03.22.713516v1.14","doi-asserted-by":"crossref","unstructured":"Costa DM , S\u00e1 M , Teixeira AR , P\u00e9rez-Cabezas B , Golba S , Sefiane-Djemaoune H , et al. 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