{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:02:56Z","timestamp":1785420176216,"version":"3.56.0"},"posted":{"date-parts":[[2026,3,25]]},"group-title":"Neuroscience","reference-count":40,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T00:00:00Z","timestamp":1774396800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"award":["R01EY019498, R01EY013528, R21EY037926, and P30EY003176"],"award-info":[{"award-number":["R01EY019498, R01EY013528, R21EY037926, and P30EY003176"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["DGE 2146752"],"award-info":[{"award-number":["DGE 2146752"]}],"id":[{"id":"https:\/\/ror.org\/021nxhr62","id-type":"ROR","asserted-by":"publisher"}]},{"name":"HHMI Gilliam Award","award":["GT17844"],"award-info":[{"award-number":["GT17844"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,3,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Coordinated signaling among neurons, glia, and the vasculature is essential for the formation of a functional nervous system, yet how these relationships emerge during development remains unclear. Here, we investigated the developmental interplay between neural activity, M\u00fcller glia, and the retina vasculature in mice. Using quantitative confocal imaging from postnatal day 5 to eye-opening, we mapped the emergence of the superficial, intermediate, and deep vasculature layers and found that they emerged normally in mice lacking the \u03b22-containing nicotinic acetylcholine receptors, despite a dramatic reduction in cholinergic signaling. Tip cell density and overall vessel growth were unchanged, indicating cholinergic wave activity is not required for the emergence of retinal vasculature.<\/jats:p>\n                <jats:p>We next defined the developmental timeline of M\u00fcller glia\u2013vascular interactions. Sparse labeling and immunohistochemistry revealed that M\u00fcller glial lateral processes closely associate with endothelial tip cells during intermediate- and deep-layer angiogenesis and establish Aquaporin-4\u2013enriched endfeet at vascular contact sites from the earliest stages of growth, even when vessel trajectories are perturbed.<\/jats:p>\n                <jats:p>Finally, two-photon calcium imaging combined with simultaneous electrophysiology demonstrated that M\u00fcller glial endfeet exhibit robust, compartmentalized calcium transients during development. Although a subset of events was temporally correlated with retinal waves, enhancing neurotransmitter spillover selectively increased wave-associated activity in glial stalks but not endfeet. These findings indicate that calcium signaling at the glial-vascular interface is largely independent of spontaneous neuronal activity. Together, our results support a model in which M\u00fcller glia engage growing vessels through an activity-independent, parallel developmental program that may provide instructive cues for retinal angiogenesis.<\/jats:p>","DOI":"10.64898\/2026.03.23.713726","type":"posted-content","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T04:27:43Z","timestamp":1774499263000},"source":"Crossref","is-referenced-by-count":0,"title":["M\u00fcller glia-vasculature interactions in the developing retina"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2910-7739","authenticated-orcid":false,"given":"Samira","family":"Monshietehadi","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3145-2846","authenticated-orcid":false,"given":"Angel 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