{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T01:52:13Z","timestamp":1780969933177,"version":"3.54.1"},"posted":{"date-parts":[[2026,3,26]]},"group-title":"Bioengineering","reference-count":41,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T00:00:00Z","timestamp":1774483200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["U01 NS100588, R01 AG085299, R01 AG071515, R01 AG091312, P41 EB031771, U24 NS141774 and P30 AG066512"],"award-info":[{"award-number":["U01 NS100588, R01 AG085299, R01 AG071515, R01 AG091312, P41 EB031771, U24 NS141774 and P30 AG066512"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,3,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Efficient metabolic waste clearance, via the postulated glymphatic system, is essential for neural homeostasis. However, direct visualization of tissue-cerebrospinal fluid (CSF) exchange remains limited, leading to ongoing debate in the neuroscientific field. The present work revealed evidence of tissue-CSF water exchange in the live human cortex, by employing a novel MRI technique demonstrating the flux of water molecules across the perivascular interface. We observed robust water exchange inside the cortical ribbon, which was more prominent than white matter and deep brain tissue. We validated that the signal originates from CSF and is independent of cerebral perfusion. Water exchange between tissue and CSF declined with age. Furthermore, we demonstrated for the first time that tissue-CSF exchange was impaired in Alzheimer\u2019s disease (AD), in particular in regions where the perivascular space is clogged by anti-amyloid immunotherapy.<\/jats:p>","DOI":"10.64898\/2026.03.26.714533","type":"posted-content","created":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T17:43:17Z","timestamp":1774806197000},"source":"Crossref","is-referenced-by-count":1,"title":["Non-invasive MRI mapping of tissue-CSF water exchange reveals glymphatic fluid movement in live human cortex"],"prefix":"10.64898","author":[{"given":"Zihan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyi","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengrong","family":"Jiang","sequence":"additional","affiliation":[{"name":"The Russell H. 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