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In our study, we employed two single-molecule methods, F\u00f6rster Resonance Energy Transfer (smFRET) and high-resolution optical tweezers, to investigate the transcription of\n                  <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                  RNA polymerase (MtbRNAP) from initiation through to termination. We aim to provide a set of comprehensive biophysical tools to deepen our current understanding of MtbRNAP and its transcription factors. These experimental assays represent an important step towards unraveling the molecular dynamics and interactions that support transcription in\n                  <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                  .\n                <\/jats:p>","DOI":"10.64898\/2026.03.27.714832","type":"posted-content","created":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T04:25:13Z","timestamp":1774758313000},"source":"Crossref","is-referenced-by-count":0,"title":["Single-Molecule Methods to Investigate Mechanisms of Transcription by RNA Polymerase of\n                  <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.64898","author":[{"given":"Wenxia","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, Guangdong 518000, China"},{"name":"Institute for Quantitative Biosciences-QB3, University of California, Berkeley, CA 94720, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omar","family":"Herrera-Asmat","sequence":"additional","affiliation":[{"name":"Institute for Quantitative Biosciences-QB3, University of California, Berkeley, CA 94720, United States"},{"name":"Department of Molecular Cell and Biology, University of California, Berkeley, CA 94720, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander B.","family":"Tong","sequence":"additional","affiliation":[{"name":"Institute for Quantitative Biosciences-QB3, University of California, Berkeley, CA 94720, United States"},{"name":"Department of Chemistry, University of California, Berkeley, CA 94720, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiantian","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, Guangdong 518000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2970-0073","authenticated-orcid":false,"given":"Carlos","family":"Bustamante","sequence":"additional","affiliation":[{"name":"Institute for Quantitative Biosciences-QB3, University of California, Berkeley, CA 94720, United States"},{"name":"Department of Molecular Cell and Biology, University of California, Berkeley, CA 94720, United States"},{"name":"Department of Chemistry, University of California, Berkeley, CA 94720, United States"},{"name":"Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, United States"},{"name":"Department of Physics, University of California, Berkeley, CA 94720, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026041708301057000_2026.03.27.714832v2.1","unstructured":"Wolrd Health Organization. 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