{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T11:59:08Z","timestamp":1775044748487,"version":"3.50.1"},"posted":{"date-parts":[[2026,3,28]]},"group-title":"Cell Biology","reference-count":44,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T00:00:00Z","timestamp":1774656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"National Heart, Lung, and Blood Institute Division of Intramural Research","award":["R01HL155921"],"award-info":[{"award-number":["R01HL155921"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,3,28]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Caveolae, flask-shaped membrane invaginations highly enriched in endothelial cells, play a central role in buffering membrane tension, yet the principles governing their spatial organization remain elusive. This investigation sought to generate the most comprehensive and systematic analysis of blood vessel caveolar spatial organization. To do so, our group leveraged micropatterning technologies to impose precise biophysical constraints on endothelial cell geometry to probe how caveolae are organized under defined tensional and polarity environments. These experiments were integrated with a high-throughput spatial cell mapping computational pipeline for analyzing thousands of caveolae, providing an extremely high-fidelity analysis. Our results provide a governing framework of how total cellular caveolae are spatially organized during random and directional migration, non-motile polarized, nascent and stable monolayers with differing confinement levels as well as in angiogenic vasculature in vivo. Broadly, our results demonstrated caveolae preferentially organized in the rear of migrating and polarized endothelial cells. In differing monolayer configurations, caveolae default to a peri-junctional spatial organization. Lastly, in mouse retinal blood vessels caveolae are most prominent in the vascular front due to their responsiveness to vascular endothelial growth factor signaling. Overall, these results strongly suggest that caveolae cellular arrangement and number are highly predictive of vascular stability and remodeling states.<\/jats:p>","DOI":"10.64898\/2026.03.27.714916","type":"posted-content","created":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T18:20:11Z","timestamp":1774722011000},"source":"Crossref","is-referenced-by-count":0,"title":["PRINCIPLES GOVERNING ENDOTHELIAL CAVEOLAE ORGANIZATION DURING ANGIOGENESIS"],"prefix":"10.64898","author":[{"given":"Drew B.","family":"Grespin","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Denver, Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jasper S.","family":"Farrington","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Denver, Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Talen G.","family":"Niven","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Denver, Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liam J.","family":"Russell","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Biophysics Programs, University of Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dinah","family":"Loerke","sequence":"additional","affiliation":[{"name":"Department of Physics and Astronomy, University of Denver, CO, USA"},{"name":"Molecular and Cellular Biophysics Programs, University of Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaryn J.","family":"David","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Denver, Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret S.","family":"Grespin","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Denver, Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris M.","family":"Culkin","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Denver, Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adella P.","family":"Bartoletti","sequence":"additional","affiliation":[{"name":"Cell and Molecular Biology Department, Tulane University, New Orleans, LA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0968-7155","authenticated-orcid":false,"given":"Stryder M.","family":"Meadows","sequence":"additional","affiliation":[{"name":"Cell and Molecular Biology Department, Tulane University, New Orleans, LA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erich J.","family":"Kushner","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Denver, Denver, CO, USA"},{"name":"Molecular and Cellular Biophysics Programs, University of Denver, CO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026040103451027000_2026.03.27.714916v1.1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000070546.16946.3A"},{"key":"2026040103451027000_2026.03.27.714916v1.2","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.3390\/biom10091218","article-title":"Caveolae and Lipid Rafts in Endothelium: Valuable Organelles for Multiple Functions","volume":"10","year":"2020","journal-title":"Biomolecules"},{"key":"2026040103451027000_2026.03.27.714916v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2808"},{"key":"2026040103451027000_2026.03.27.714916v1.4","doi-asserted-by":"publisher","DOI":"10.1172\/JCI27100"},{"key":"2026040103451027000_2026.03.27.714916v1.5","doi-asserted-by":"crossref","unstructured":"Grivas, D. , Gonz\u00e1lez-Rajal, \u00c1. , Guerrero Rodr\u00edguez, C. , Garcia, R. & de la Pompa, J.L . 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