{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T19:55:25Z","timestamp":1775159725408,"version":"3.50.1"},"posted":{"date-parts":[[2026,3,31]]},"group-title":"Neuroscience","reference-count":45,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["R01NS113915"],"award-info":[{"award-number":["R01NS113915"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"crossref","award":["R01NS123708"],"award-info":[{"award-number":["R01NS123708"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,3,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Adult zebrafish exhibit scarless repair and functional recovery following spinal cord injury. Their regenerative capacity is attributed to potent stem-like progenitors that mediate neuronal and glial repair. Zebrafish are thought to lack anti-regenerative extracellular matrix (ECM) components abundant in mammalian SCI, but the positive contributions of ECM to spontaneous spinal cord repair are less understood. By employing cross-species single-cell transcriptomics, we found the hyaluran modifying enzyme Hapln1 is upregulated in zebrafish progenitors but not in mouse progenitors following injury. Loss-of-function of\n                  <jats:italic>hapln1a\/b<\/jats:italic>\n                  and ablation of\n                  <jats:italic>hapln1<\/jats:italic>\n                  <jats:sup>+<\/jats:sup>\n                  cells reduce progenitor cell activation and hinder spontaneous recovery from injury. Using a series of\n                  <jats:italic>in vivo<\/jats:italic>\n                  and\n                  <jats:italic>in vitro<\/jats:italic>\n                  assays, we show that Hapln1 is required for hyaluran-\n                  <jats:italic>cd44b<\/jats:italic>\n                  mediated progenitor cell proliferation. This study reveals that, in addition to lacking anti-regenerative ECM components around SC lesions, zebrafish can also leverage pro-regenerative ECM molecules to enhance progenitor cell potency and promote repair.\n                <\/jats:p>","DOI":"10.64898\/2026.03.28.714985","type":"posted-content","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T18:35:43Z","timestamp":1774982143000},"source":"Crossref","is-referenced-by-count":0,"title":["Hapln1-HA signaling promotes progenitor cell proliferation and spinal cord regeneration"],"prefix":"10.64898","author":[{"given":"Yuxiao","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Developmental Biology, Washington University School of Medicine, Saint Louis, MO, USA"},{"name":"Center of Regenerative Medicine, Washington University School of Medicine, Saint Louis, MO, USA"},{"name":"Hope Center for Neurological Disorders, Washington University School of Medicine, Saint Louis, MO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lili","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Washington University School of Medicine, Saint Louis, MO, USA"},{"name":"Center of Regenerative Medicine, Washington University School of Medicine, Saint Louis, MO, USA"},{"name":"Hope Center for Neurological Disorders, Washington University School of Medicine, Saint Louis, MO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishnu","family":"Saraswathy","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Washington University School of Medicine, Saint Louis, MO, USA"},{"name":"Center of Regenerative Medicine, Washington University School of Medicine, Saint Louis, MO, USA"},{"name":"Hope Center for Neurological Disorders, Washington University School of Medicine, Saint Louis, MO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan","family":"McAdow","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Washington University School of Medicine, Saint Louis, MO, USA"},{"name":"Center of Regenerative Medicine, Washington University School of Medicine, Saint Louis, MO, USA"},{"name":"Hope Center for Neurological Disorders, Washington University School of Medicine, Saint Louis, MO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3712-351X","authenticated-orcid":false,"given":"Mayssa H.","family":"Mokalled","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Washington University School of Medicine, Saint Louis, MO, USA"},{"name":"Center of Regenerative Medicine, Washington University School of Medicine, Saint Louis, MO, USA"},{"name":"Hope Center for Neurological Disorders, Washington University School of Medicine, Saint Louis, MO, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026040212101450000_2026.03.28.714985v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2010.07.014"},{"issue":"2-3","key":"2026040212101450000_2026.03.28.714985v1.2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.3233\/RNN-2008-00443","article-title":"Adult zebrafish as a model for successful central nervous system regeneration","volume":"26","year":"2008","journal-title":"Restorative neurology and neuroscience"},{"key":"2026040212101450000_2026.03.28.714985v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/416636a"},{"key":"2026040212101450000_2026.03.28.714985v1.4","doi-asserted-by":"crossref","unstructured":"Burris, B. , Jensen, N. and Mokalled, M. 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