{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:27:10Z","timestamp":1785421630994,"version":"3.56.0"},"posted":{"date-parts":[[2026,4,1]]},"reference-count":50,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"award":["EY-033308"],"award-info":[{"award-number":["EY-033308"]}],"id":[{"id":"https:\/\/ror.org\/03wkg3b53","id-type":"ROR","asserted-by":"publisher"}]},{"award":["EY-031923"],"award-info":[{"award-number":["EY-031923"]}],"id":[{"id":"https:\/\/ror.org\/03wkg3b53","id-type":"ROR","asserted-by":"publisher"}]},{"award":["EY-025580"],"award-info":[{"award-number":["EY-025580"]}],"id":[{"id":"https:\/\/ror.org\/03wkg3b53","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,6,23]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Age-related macular degeneration (AMD) is a common, complex disease affecting older individuals that can lead to severe vision loss. It is characterized by early anatomical changes in the retina, retinal pigment epithelium (RPE), and choroid, especially in the central (macular) region. AMD can progress to severe atrophy and\/or pathologic angiogenesis that leads to visual decline. Over 30 genetic loci have been identified as contributing to AMD risk; however, the mechanisms by which genetic variants affect pathology has not been thoroughly explored. In this report we examined single-nucleus gene expression in the retina, RPE and choroid of 88 individuals categorized by AMD stage, as well as 36 previously published samples. Genotyping was performed on 1.8 million SNPs, with additional SNPs imputed, on each donor to identify expression quantitative trait loci (eQTLs). We found that two AMD-risk loci (PILRB and ARMS2\/HTRA1) affected the expression of\n                  <jats:italic>PILRB<\/jats:italic>\n                  and\n                  <jats:italic>HTRA1<\/jats:italic>\n                  , respectively. The risk allele of PILRB was associated with increased\n                  <jats:italic>PILRB<\/jats:italic>\n                  RNA in cones, fibroblasts, choroidal resident macrophages, and RPE, whereas the HTRA1 risk locus was associated with decreased\n                  <jats:italic>HTRA1<\/jats:italic>\n                  RNA in the RPE. We also identified an age-related decrease in complement inhibitors in the choriocapillaris, a tissue susceptible to complement mediated damage in AMD. We have made the eQTL and gene expression data fully available on the web resource Spectacle for rapid and interactive data access.\n                <\/jats:p>","DOI":"10.64898\/2026.03.30.714946","type":"posted-content","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T15:45:19Z","timestamp":1775058319000},"source":"Crossref","is-referenced-by-count":0,"title":["Single-cell quantitative trait loci analyses identify expression changes in macular degeneration risk genes"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8107-8317","authenticated-orcid":false,"given":"Andrew P.","family":"Voigt","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA, 52242"},{"name":"Institute for Vision Research, the University of Iowa, Iowa City, IA, 52242"},{"name":"Department of Ophthalmology, Northwestern University, Chicago IL 60611"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathaniel K.","family":"Mullin","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA, 52242"},{"name":"Institute for Vision Research, the University of Iowa, Iowa City, IA, 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Mulfaul","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA, 52242"},{"name":"Institute for Vision Research, the University of Iowa, Iowa City, IA, 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lola P.","family":"Lozano","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA, 52242"},{"name":"Institute for Vision Research, the University of Iowa, Iowa City, IA, 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma M.","family":"Navratil","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA, 52242"},{"name":"Institute for Vision Research, the University of Iowa, Iowa City, IA, 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miles J.","family":"Flamme-Wiese","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA, 52242"},{"name":"Institute for Vision Research, the University of Iowa, Iowa City, IA, 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy A.","family":"Lavine","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Northwestern University, Chicago IL 60611"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John H.","family":"Fingert","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA, 52242"},{"name":"Institute for Vision Research, the University of Iowa, Iowa City, IA, 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Budd A.","family":"Tucker","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA, 52242"},{"name":"Institute for Vision Research, the University of Iowa, Iowa City, IA, 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin M.","family":"Stone","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA, 52242"},{"name":"Institute for Vision Research, the University of Iowa, Iowa City, IA, 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd E.","family":"Scheetz","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA, 52242"},{"name":"Institute for Vision Research, the University of Iowa, Iowa City, IA, 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert F.","family":"Mullins","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, the University of Iowa Carver College of Medicine, Iowa City, IA, 52242"},{"name":"Institute for Vision Research, the University of Iowa, Iowa City, IA, 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