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Current\n                  <jats:italic>in vitro<\/jats:italic>\n                  models address this by fusing independently generated organoids, but this introduces variability in size, maturation state, and connectivity, confounding the study of regionalization itself. Here, we present a microfluidic platform that supports the co-development of different tissue identities within a single, continuous 3D culture domain. The device integrates controlled microfluidic flow with real-time fluorescence imaging, providing stable perfusion and high-resolution tracking of molecular transport without the need for embedded sensors or disruptive sampling. By delivering SAG, a Sonic hedgehog pathway agonist, to one surface of mouse forebrain organoids, we induced spatially segregated ventral (Nkx2.1\n                  <jats:sup>+<\/jats:sup>\n                  ) and dorsal (Pax6\n                  <jats:sup>+<\/jats:sup>\n                  ) domains within a unified tissue architecture. Controlled morphogen delivery is sufficient to drive region-specific fate specification without organoid fusion, offering a practical, scalable alternative for studying tissue regionalization\n                  <jats:italic>in vitro<\/jats:italic>\n                  .\n                <\/jats:p>","DOI":"10.64898\/2026.04.05.716567","type":"posted-content","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T19:40:18Z","timestamp":1775677218000},"source":"Crossref","is-referenced-by-count":0,"title":["Microfluidic Control of Dorsal-Ventral Patterning Within a Single Forebrain Organoid"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4900-7161","authenticated-orcid":false,"given":"Sebastian","family":"Torres-Montoya","sequence":"first","affiliation":[{"name":"Genomics Institute, University of California, Santa Cruz, CA 95060, United States"},{"name":"Department of Electrical and Computer Engineering, University of California, Santa Cruz, CA 95064, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9554-5617","authenticated-orcid":false,"given":"Samira","family":"Vera-Choqqueccota","sequence":"additional","affiliation":[{"name":"Genomics Institute, University of California, Santa Cruz, CA 95060, United States"},{"name":"Department of Biomolecular Engineering, University of California, Santa Cruz, CA 95064, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0087-1346","authenticated-orcid":false,"given":"Spencer T.","family":"Seiler","sequence":"additional","affiliation":[{"name":"Genomics Institute, University of California, Santa Cruz, CA 95060, United States"},{"name":"Department of Biomolecular Engineering, University of California, Santa Cruz, CA 95064, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1533-4575","authenticated-orcid":false,"given":"David","family":"Haussler","sequence":"additional","affiliation":[{"name":"Genomics Institute, University of California, Santa Cruz, CA 95060, United States"},{"name":"Department of Biomolecular Engineering, University of California, Santa Cruz, CA 95064, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6999-7193","authenticated-orcid":false,"given":"Sofie R.","family":"Salama","sequence":"additional","affiliation":[{"name":"Genomics Institute, University of California, Santa Cruz, CA 95060, United States"},{"name":"Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1634-7514","authenticated-orcid":false,"given":"Mohammed A.","family":"Mostajo-Radji","sequence":"additional","affiliation":[{"name":"Genomics Institute, University of California, Santa Cruz, CA 95060, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7085-5248","authenticated-orcid":false,"given":"Mircea","family":"Teodorescu","sequence":"additional","affiliation":[{"name":"Genomics Institute, University of California, Santa Cruz, CA 95060, United States"},{"name":"Department of Electrical and Computer Engineering, University of California, Santa Cruz, CA 95064, United States"},{"name":"Department of Biomolecular Engineering, University of California, Santa Cruz, CA 95064, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026041105150976000_2026.04.05.716567v1.1","doi-asserted-by":"crossref","first-page":"101671","DOI":"10.1016\/j.iot.2025.101671","article-title":"A feedback-driven brain organoid platform enables automated maintenance and high-resolution neural activity monitoring","volume":"33","year":"2025","journal-title":"Internet Things"},{"key":"2026041105150976000_2026.04.05.716567v1.2","first-page":"e62756","article-title":"Generation of Human Brain Organoids for Mitochondrial Disease Modeling","volume":"172","year":"2021","journal-title":"J. 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