{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:03:39Z","timestamp":1785409419340,"version":"3.56.0"},"posted":{"date-parts":[[2026,4,19]]},"group-title":"Neuroscience","reference-count":65,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T00:00:00Z","timestamp":1776556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["NS110905"],"award-info":[{"award-number":["NS110905"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"award":["HT9425-23-1-1003"],"award-info":[{"award-number":["HT9425-23-1-1003"]}],"id":[{"id":"https:\/\/ror.org\/0447fe631","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016030","name":"International Center for Responsible Gaming","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100016030","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ohio State University","award":["Center for Brain Injury Research and Discovery SURF"],"award-info":[{"award-number":["Center for Brain Injury Research and Discovery SURF"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,4,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Traumatic brain injuries (TBIs) are more than mere lesions and generate a persistent secondary pathology. This, combined with functional reorganization of circuits post-injury, may explain the increased risk for psychiatric disorders in patients with TBI. In the current studies, we demonstrate that frontal TBI changed the Pavlovian behavioral response to reinforcer-predicting cues and reduced the motivational value of cues. TBI also chronically impaired decision-making on a gambling-like task with reinforcer-paired cues. To investigate how these changes occur, we evaluated the nucleus accumbens (NAc) core. At a subacute time point (14 days), we confirmed reduced input to the NAc with optogenetics and evaluated electrophysiological and transcriptional changes. TBI increased neuronal excitability and the single nucleus RNA sequencing profile indicated a substantial stress and inflammatory response, but also high indicators of plasticity, particularly in D1- and D2-positive medium spiny neurons. To evaluate how these subacute changes transitioned to chronic NAc dysfunction, we measured immunohistochemical surrogates of activity post-mortem and recorded calcium activity from the NAc after TBI during Pavlovian conditioning. TBI reduced histological markers of activity and reduced cue-evoked calcium activity. Overall, these data indicate that substantial reorganization of the NAc occurs following frontal brain injury. A primary effect of this is to reduce the salience of environmental cues linked to outcomes. The inability to properly process outcomes could contribute to broader psychiatric symptoms after TBI, including impairments in decision-making, behavioral flexibility, and impulsivity but also presents a potential treatment target.<\/jats:p>","DOI":"10.64898\/2026.04.17.718474","type":"posted-content","created":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T10:45:13Z","timestamp":1776595513000},"source":"Crossref","is-referenced-by-count":0,"title":["Frontal Brain Injury Reduces Sensitivity to Reward-Predictive Cues and Remodels the Nucleus Accumbens"],"prefix":"10.64898","author":[{"given":"Erskine","family":"Chu","sequence":"first","affiliation":[{"name":"Center for Brain Injury Recovery and Discovery, Ohio State University, Columbus, OH, USA"},{"name":"Institute for Brain, Behavior, and Immunology, Ohio State University, Columbus, OH, USA"},{"name":"Department of Neuroscience, Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenna E.","family":"McCloskey","sequence":"additional","affiliation":[{"name":"Center for Brain Injury Recovery and Discovery, Ohio State University, Columbus, OH, USA"},{"name":"Institute for Brain, Behavior, and Immunology, Ohio State University, Columbus, OH, USA"},{"name":"Department of Neuroscience, Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mia A.","family":"Eleid","sequence":"additional","affiliation":[{"name":"Center for Brain Injury Recovery and Discovery, Ohio State University, Columbus, OH, USA"},{"name":"Institute for Brain, Behavior, and Immunology, Ohio State University, Columbus, OH, USA"},{"name":"Department of Neuroscience, Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sathvik","family":"Jami","sequence":"additional","affiliation":[{"name":"Center for Brain Injury Recovery and Discovery, Ohio State University, Columbus, OH, USA"},{"name":"Institute for Brain, Behavior, and Immunology, Ohio State University, Columbus, OH, USA"},{"name":"Department of Neuroscience, Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra G.","family":"Dorinsky","sequence":"additional","affiliation":[{"name":"Center for Brain Injury Recovery and Discovery, Ohio State University, Columbus, OH, USA"},{"name":"Institute for Brain, Behavior, and Immunology, Ohio State University, Columbus, OH, USA"},{"name":"Department of Neuroscience, Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fikir B.","family":"Arega","sequence":"additional","affiliation":[{"name":"Center for Brain Injury Recovery and Discovery, Ohio State University, Columbus, OH, USA"},{"name":"Institute for Brain, Behavior, and Immunology, Ohio State University, Columbus, OH, USA"},{"name":"Department of Neuroscience, Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kris M.","family":"Martens","sequence":"additional","affiliation":[{"name":"Center for Brain Injury Recovery and Discovery, Ohio State University, Columbus, OH, USA"},{"name":"Institute for Brain, Behavior, and Immunology, Ohio State University, Columbus, OH, USA"},{"name":"Department of Neuroscience, Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangli","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan M.","family":"Packer","sequence":"additional","affiliation":[{"name":"Center for Brain Injury Recovery and Discovery, Ohio State University, Columbus, OH, USA"},{"name":"Institute for Brain, Behavior, and Immunology, Ohio State University, Columbus, OH, USA"},{"name":"Department of Neuroscience, Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Stevens","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics, Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maciej","family":"Pietrzak","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics, Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Candice C.","family":"Askwith","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan P.","family":"Godbout","sequence":"additional","affiliation":[{"name":"Center for Brain Injury Recovery and Discovery, Ohio State University, Columbus, OH, USA"},{"name":"Institute for Brain, Behavior, and Immunology, Ohio State University, Columbus, OH, USA"},{"name":"Department of Neuroscience, Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7949-2068","authenticated-orcid":false,"given":"Cole","family":"Vonder Haar","sequence":"additional","affiliation":[{"name":"Center for Brain Injury Recovery and Discovery, Ohio State University, Columbus, OH, USA"},{"name":"Institute for Brain, Behavior, and Immunology, Ohio State University, Columbus, OH, USA"},{"name":"Department of Neuroscience, Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"issue":"11","key":"2026042109101122000_2026.04.17.718474v1.1","doi-asserted-by":"crossref","first-page":"1984","DOI":"10.2174\/1570159X19666210215123914","article-title":"The complexity of secondary cascade consequent to traumatic brain injury: Pathobiology and potential treatments","volume":"19","year":"2021","journal-title":"Current Neuropharmacology"},{"key":"2026042109101122000_2026.04.17.718474v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2012.06.008"},{"issue":"3","key":"2026042109101122000_2026.04.17.718474v1.3","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1038\/nrneurol.2014.15","article-title":"Network dysfunction after traumatic brain injury","volume":"10","year":"2014","journal-title":"Nature Reviews Neurology"},{"key":"2026042109101122000_2026.04.17.718474v1.4","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awq388"},{"key":"2026042109101122000_2026.04.17.718474v1.5","doi-asserted-by":"publisher","DOI":"10.1017\/S135561771200118X"},{"issue":"8","key":"2026042109101122000_2026.04.17.718474v1.6","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.3109\/02699052.2014.896943","article-title":"Impaired decision-making after traumatic brain injury: The Iowa Gambling Task","volume":"28","year":"2014","journal-title":"Brain Injury"},{"key":"2026042109101122000_2026.04.17.718474v1.7","doi-asserted-by":"publisher","DOI":"10.1080\/0269905011008803"},{"issue":"5","key":"2026042109101122000_2026.04.17.718474v1.8","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1080\/09602011.2010.495245","article-title":"Assessment of impulsivity after moderate to severe traumatic brain injury","volume":"20","year":"2010","journal-title":"Neuropsychological Rehabilitation"},{"key":"2026042109101122000_2026.04.17.718474v1.9","doi-asserted-by":"publisher","DOI":"10.1007\/s00127-018-1583-1"},{"issue":"10","key":"2026042109101122000_2026.04.17.718474v1.10","doi-asserted-by":"crossref","first-page":"e0239661","DOI":"10.1371\/journal.pone.0239661","article-title":"Traumatic brain injuries and problem gambling in youth: Evidence from a population-based study of secondary students in Ontario, Canada","volume":"15","year":"2020","journal-title":"PLOS ONE"},{"issue":"5","key":"2026042109101122000_2026.04.17.718474v1.11","first-page":"421","article-title":"Does traumatic brain injury cause risky substance use or substance use disorder?","volume":"91","year":"2021","journal-title":"Biological Psychiatry"},{"key":"2026042109101122000_2026.04.17.718474v1.12","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2023.0448"},{"key":"2026042109101122000_2026.04.17.718474v1.13","doi-asserted-by":"crossref","first-page":"837654","DOI":"10.3389\/fnbeh.2022.837654","article-title":"Large-N rat data enables phenotyping of risky decision-making: A retrospective analysis of brain injury on the Rodent Gambling Task","volume":"16","year":"2022","journal-title":"Frontiers in Behavioral Neuroscience"},{"key":"2026042109101122000_2026.04.17.718474v1.14","doi-asserted-by":"crossref","first-page":"112879","DOI":"10.1016\/j.bbr.2020.112879","article-title":"Choice-based assessments outperform traditional measures for chronic depressive-like behaviors in rats after brain injury","volume":"395","year":"2020","journal-title":"Behavioural Brain Research"},{"key":"2026042109101122000_2026.04.17.718474v1.15","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2016.4813"},{"key":"2026042109101122000_2026.04.17.718474v1.16","doi-asserted-by":"publisher","DOI":"10.1037\/bne0000590"},{"key":"2026042109101122000_2026.04.17.718474v1.17","doi-asserted-by":"crossref","first-page":"147084","DOI":"10.1016\/j.brainres.2020.147084","article-title":"Traumatic brain injury substantially reduces the conditioned reinforcing effects of environmental cues in rats","volume":"1748","year":"2020","journal-title":"Brain Research"},{"issue":"1","key":"2026042109101122000_2026.04.17.718474v1.18","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/s00429-011-0331-z","article-title":"Glutamatergic input from specific sources influences the nucleus accumbens-ventral pallidum information flow","volume":"217","year":"2012","journal-title":"Brain Structure and Function"},{"key":"2026042109101122000_2026.04.17.718474v1.19","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0848-23.2023"},{"key":"2026042109101122000_2026.04.17.718474v1.20","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3191-17.2018"},{"key":"2026042109101122000_2026.04.17.718474v1.21","doi-asserted-by":"publisher","DOI":"10.1093\/ijnp\/pyv043"},{"issue":"8","key":"2026042109101122000_2026.04.17.718474v1.22","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1093\/gerona\/glad079","article-title":"Significant acceleration of regional brain aging and atrophy after mild traumatic brain injury","volume":"78","year":"2023","journal-title":"The Journals of Gerontology Series A: Biological Sciences Medical Sciences"},{"issue":"1","key":"2026042109101122000_2026.04.17.718474v1.23","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1089\/neur.2020.0031","article-title":"Cognitive dysfunction, brain volumes, and traumatic brain injury in homeless persons","volume":"2","year":"2021","journal-title":"Neurotrauma Reports"},{"issue":"2","key":"2026042109101122000_2026.04.17.718474v1.24","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1007\/s11682-018-9854-8","article-title":"Subcortical shape and neuropsychological function among U.S. service members with mild traumatic brain injury","volume":"13","year":"2019","journal-title":"Brain Imaging and Behavior"},{"issue":"2","key":"2026042109101122000_2026.04.17.718474v1.25","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1007\/s00062-022-01233-3","article-title":"Deep grey matter volume is reduced in amateur boxers as compared to healthy age-matched controls","volume":"33","year":"2023","journal-title":"Clinical Neuroradiolology"},{"issue":"2","key":"2026042109101122000_2026.04.17.718474v1.26","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1227\/NEU.0000000000001190","article-title":"Improved function after deep brain stimulation for chronic, severe traumatic brain injury","volume":"79","year":"2016","journal-title":"Neurosurgery"},{"issue":"4","key":"2026042109101122000_2026.04.17.718474v1.27","doi-asserted-by":"crossref","first-page":"636","DOI":"10.9758\/cpn.2020.18.4.636","article-title":"Successful treatment of psychiatric symptoms after traumatic brain injury using deep brain stimulation to the anterior limb of internal capsule-nucleus accumbens","volume":"18","year":"2020","journal-title":"Clinical Psychopharmacology and Neuroscience"},{"key":"2026042109101122000_2026.04.17.718474v1.28","doi-asserted-by":"publisher","DOI":"10.1037\/amp0000059"},{"key":"2026042109101122000_2026.04.17.718474v1.29","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2225-15.2016"},{"key":"2026042109101122000_2026.04.17.718474v1.30","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3477-17.2018"},{"key":"2026042109101122000_2026.04.17.718474v1.31","doi-asserted-by":"crossref","unstructured":"Hathaway, B.A. , et al., Audiovisual cues must be predictable and win-paired to drive risky choice. bioRxiv, 2022: p. 2022.09.20.507379.","DOI":"10.1101\/2022.09.20.507379"},{"key":"2026042109101122000_2026.04.17.718474v1.32","doi-asserted-by":"publisher","DOI":"10.1038\/s41386-023-01592-6"},{"key":"2026042109101122000_2026.04.17.718474v1.33","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbrainres.2004.05.014"},{"key":"2026042109101122000_2026.04.17.718474v1.34","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1875-13.2013"},{"key":"2026042109101122000_2026.04.17.718474v1.35","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2097-21.2022"},{"key":"2026042109101122000_2026.04.17.718474v1.36","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.05.042"},{"key":"2026042109101122000_2026.04.17.718474v1.37","first-page":"1","article-title":"The neurobiology of activational aspects of motivation: Exertion of effort, effort-based decision making, and the role of dopamine","volume":"75","year":"2024","journal-title":"Annual Reviews of Psychology"},{"issue":"1","key":"2026042109101122000_2026.04.17.718474v1.38","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1038\/s41467-021-21246-9","article-title":"Inference and analysis of cell-cell communication using CellChat","volume":"12","year":"2021","journal-title":"Nature Communications"},{"issue":"1","key":"2026042109101122000_2026.04.17.718474v1.39","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1186\/s12974-023-02831-9","article-title":"Ambient RNAs removal of cortex-specific snRNA-seq reveals Apoe(+) microglia\/macrophage after deeper cerebral hypoperfusion in mice","volume":"20","year":"2023","journal-title":"Journal of Neuroinflammation"},{"key":"2026042109101122000_2026.04.17.718474v1.40","doi-asserted-by":"publisher","DOI":"10.1111\/adb.13250"},{"issue":"1","key":"2026042109101122000_2026.04.17.718474v1.41","doi-asserted-by":"crossref","first-page":"18258","DOI":"10.1038\/s41598-024-69255-0","article-title":"A single-nucleus transcriptomic atlas of medium spiny neurons in the rat nucleus accumbens","volume":"14","year":"2024","journal-title":"Scientific Reports"},{"key":"2026042109101122000_2026.04.17.718474v1.42","doi-asserted-by":"crossref","unstructured":"Fitzgerald, E. , et al., Sex and cell-specific gene expression in corticolimbic brain regions associated with psychiatric disorders revealed by bulk and single-nuclei RNA sequencing. eBioMedicine, 2023. 95.","DOI":"10.1016\/j.ebiom.2023.104749"},{"key":"2026042109101122000_2026.04.17.718474v1.43","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(01)02912-2"},{"key":"2026042109101122000_2026.04.17.718474v1.44","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.11.021"},{"key":"2026042109101122000_2026.04.17.718474v1.45","doi-asserted-by":"publisher","DOI":"10.1152\/jn.91002.2008"},{"key":"2026042109101122000_2026.04.17.718474v1.46","doi-asserted-by":"publisher","DOI":"10.1126\/science.1060818"},{"key":"2026042109101122000_2026.04.17.718474v1.47","doi-asserted-by":"publisher","DOI":"10.1126\/science.1137073"},{"issue":"2","key":"2026042109101122000_2026.04.17.718474v1.48","first-page":"181","article-title":"Reward or reinforcement: What\u2019s the difference?","volume":"13","year":"1989","journal-title":"Neuroscience & Biobehavioral Reviews"},{"key":"2026042109101122000_2026.04.17.718474v1.49","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5306.1593"},{"key":"2026042109101122000_2026.04.17.718474v1.50","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.05.011"},{"key":"2026042109101122000_2026.04.17.718474v1.51","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-019-0484-3"},{"issue":"5","key":"2026042109101122000_2026.04.17.718474v1.52","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1016\/j.neuron.2023.11.023","article-title":"D1 and D2 medium spiny neurons in the nucleus accumbens core have distinct and valence-independent roles in learning","volume":"112","year":"2024","journal-title":"Neuron"},{"key":"2026042109101122000_2026.04.17.718474v1.53","doi-asserted-by":"crossref","unstructured":"Vonder Haar, C. , et al., Cocaine self-administration is increased after frontal traumatic brain injury and associated with neuroinflammation. European Journal of Neuroscience, 2019. 50(3).","DOI":"10.1111\/ejn.14123"},{"issue":"1","key":"2026042109101122000_2026.04.17.718474v1.54","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1089\/neu.2015.4275","article-title":"Adolescent traumatic brain injury induces chronic mesolimbic neuroinflammation with concurrent enhancement in the rewarding effects of cocaine in mice during adulthood","volume":"34","year":"2017","journal-title":"Journal of Neurotrauma"},{"issue":"1","key":"2026042109101122000_2026.04.17.718474v1.55","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1089\/neu.2019.6472","article-title":"Experimental traumatic brain injury during adolescence enhances cocaine rewarding efficacy and dysregulates dopamine and neuroimmune systems in brain reward substrates","volume":"37","year":"2020","journal-title":"Journal of Neurotrauma"},{"key":"2026042109101122000_2026.04.17.718474v1.56","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aba4221"},{"key":"2026042109101122000_2026.04.17.718474v1.57","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.ads4441"},{"key":"2026042109101122000_2026.04.17.718474v1.58","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2009"},{"issue":"10","key":"2026042109101122000_2026.04.17.718474v1.59","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1016\/j.biopsych.2023.08.006","article-title":"Operant training for highly palatable food alters translating messenger RNA in nucleus accumbens D(2) neurons and reveals a modulatory role of Ncdn","volume":"95","year":"2024","journal-title":"Biological Psychiatry"},{"key":"2026042109101122000_2026.04.17.718474v1.60","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-021-05887-8"},{"key":"2026042109101122000_2026.04.17.718474v1.61","doi-asserted-by":"publisher","DOI":"10.1089\/neu.1994.11.417"},{"key":"2026042109101122000_2026.04.17.718474v1.62","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-006-0535-8"},{"issue":"1","key":"2026042109101122000_2026.04.17.718474v1.63","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1186\/s12974-025-03451-1","article-title":"Diffuse traumatic brain injury induced stimulator of interferons (STING) signaling in microglia drives cortical neuroinflammation, neuronal dysfunction, and impaired cognition","volume":"22","year":"2025","journal-title":"Journal of Neuroinflammation"},{"key":"2026042109101122000_2026.04.17.718474v1.64","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"issue":"1","key":"2026042109101122000_2026.04.17.718474v1.65","doi-asserted-by":"crossref","first-page":"5692","DOI":"10.1038\/s41467-021-25960-2","article-title":"Confronting false discoveries in single-cell differential expression","volume":"12","year":"2021","journal-title":"Nature Communications"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.04.17.718474","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T16:10:28Z","timestamp":1776787828000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.04.17.718474"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,19]]},"references-count":65,"URL":"https:\/\/doi.org\/10.64898\/2026.04.17.718474","relation":{},"subject":[],"published":{"date-parts":[[2026,4,19]]},"subtype":"preprint"}}