{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T23:47:34Z","timestamp":1787701654476,"version":"build-2784847793"},"posted":{"date-parts":[[2026,4,27]]},"group-title":"Bioengineering","reference-count":0,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"award":["R21 DK136026 (J.C.V.)"],"award-info":[{"award-number":["R21 DK136026 (J.C.V.)"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01 DK135716 (S.J.S, J.C.V.)"],"award-info":[{"award-number":["R01 DK135716 (S.J.S, J.C.V.)"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01 CA244170 (J.T.C.L.)"],"award-info":[{"award-number":["R01 CA244170 (J.T.C.L.)"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01 EB031002 (J.T.C.L.)"],"award-info":[{"award-number":["R01 EB031002 (J.T.C.L.)"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01 CA268207 (J.T.C.L.)"],"award-info":[{"award-number":["R01 CA268207 (J.T.C.L.)"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01 DK138948 (J.T.C.L.)"],"award-info":[{"award-number":["R01 DK138948 (J.T.C.L.)"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["Indiana O\u0092Brien U54 DK137328 (P.C.D, J.C.V., J.T.C.L.)"],"award-info":[{"award-number":["Indiana O\u0092Brien U54 DK137328 (P.C.D, J.C.V., J.T.C.L.)"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["TL1DK143270 (P.V.)"],"award-info":[{"award-number":["TL1DK143270 (P.V.)"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["NIDDK Innovative Science Accelerator Program ISAC DK128851 (C.P.)"],"award-info":[{"award-number":["NIDDK Innovative Science Accelerator Program ISAC DK128851 (C.P.)"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["Postdoctoral fellowships (C.P., D.B.)"],"award-info":[{"award-number":["Postdoctoral fellowships (C.P., D.B.)"]}],"id":[{"id":"https:\/\/ror.org\/00hasdx88","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,4,27]]},"abstract":"<jats:p>Advances in tissue clearing and lightsheet microscopy enable mesoscale imaging of intact and convoluted tubular networks, yet analytical tools to map tubule continuity and assess injury patterns within and across tubules are limited. Here, we introduce TubuleMAP, a semi-automated pipeline for 3D tubule tracking and reconstruction that adapts to various morphological and staining patterns, leverages parallel processing of terabyte-scale data for large-scale analysis of tubular networks, and uses a napari interface for human oversight. Using TubuleMAP, we reconstruct 1,000 intact mouse nephrons in ~1-millimeter-thick kidney slab with ~400-fold higher throughput and &lt;1% human effort compared to prior approaches. These reconstructions enable analysis of mesoscale nephron organization, quantitative profiling of pathologic morphologies, whole-nephron cytometry, and identification of rare morphologies at unprecedented scales. We demonstrate generalizability by reconstructing all seminiferous tubules in a mouse testis within a day. TubuleMAP is released as an open-source Python package.<\/jats:p>","DOI":"10.64898\/2026.04.23.720231","type":"posted-content","created":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T23:25:13Z","timestamp":1777332313000},"source":"Crossref","is-referenced-by-count":1,"title":["High-throughput, organ-scale 3D tubule tracking using TubuleMAP"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8512-9238","authenticated-orcid":false,"given":"Chetan","family":"Poudel","sequence":"first","affiliation":[{"name":"Indiana University School of Medicine;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1969-5386","authenticated-orcid":false,"given":"David","family":"Brenes","sequence":"additional","affiliation":[{"name":"Stanford University;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhui","family":"Xie","sequence":"additional","affiliation":[{"name":"University of Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Guan","sequence":"additional","affiliation":[{"name":"University of Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Vasquez","sequence":"additional","affiliation":[{"name":"University of Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naren","family":"Mahadevan","sequence":"additional","affiliation":[{"name":"University of Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eesha","family":"Konatham","sequence":"additional","affiliation":[{"name":"University of Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Fan","sequence":"additional","affiliation":[{"name":"University of Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1200-7328","authenticated-orcid":false,"given":"Ziyu","family":"Guo","sequence":"additional","affiliation":[{"name":"University of Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas J","family":"Porter","sequence":"additional","affiliation":[{"name":"University of Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeline K","family":"Wong","sequence":"additional","affiliation":[{"name":"University of Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle M.","family":"Martinez","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruben M","family":"Sandoval","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth W","family":"Dunn","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre C","family":"Dagher","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana G","family":"Eng","sequence":"additional","affiliation":[{"name":"University of Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart J","family":"Shankland","sequence":"additional","affiliation":[{"name":"University of Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan T.C.","family":"Liu","sequence":"additional","affiliation":[{"name":"Stanford University;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6550-8935","authenticated-orcid":false,"given":"Joshua C.","family":"Vaughan","sequence":"additional","affiliation":[{"name":"University of Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.04.23.720231","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T23:25:13Z","timestamp":1777332313000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.04.23.720231"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,27]]},"references-count":0,"URL":"https:\/\/doi.org\/10.64898\/2026.04.23.720231","relation":{},"subject":[],"published":{"date-parts":[[2026,4,27]]},"subtype":"preprint"}}