{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:34:37Z","timestamp":1785429277486,"version":"3.56.0"},"posted":{"date-parts":[[2026,5,1]]},"group-title":"Neuroscience","reference-count":67,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["U01EY025501"],"award-info":[{"award-number":["U01EY025501"]}],"id":[{"id":"https:\/\/ror.org\/03wkg3b53","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01EY029710"],"award-info":[{"award-number":["R01EY029710"]}],"id":[{"id":"https:\/\/ror.org\/03wkg3b53","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01EY018139"],"award-info":[{"award-number":["R01EY018139"]}],"id":[{"id":"https:\/\/ror.org\/03wkg3b53","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01EY017607"],"award-info":[{"award-number":["R01EY017607"]}],"id":[{"id":"https:\/\/ror.org\/03wkg3b53","id-type":"ROR","asserted-by":"publisher"}]},{"award":["P30EY001730"],"award-info":[{"award-number":["P30EY001730"]}],"id":[{"id":"https:\/\/ror.org\/03wkg3b53","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/04drjs621","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Gene & Ruth Posner Foundation"},{"name":"George and Martina Kren Endowed KEMI chair in Vision Research"},{"name":"Kren Engineering in Medicine Initiative"},{"name":"SPIE Franz-Hillenkamp Postdoctoral Fellowship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Human cone and rod outer segments exhibit a rapid shrinkage followed by a slower elongation in response to light, forming the basis of the optoretinogram. The molecular basis of this optical assay of photoreceptor function remains incompletely understood. In mouse rods, the elongation response requires transducin, the G-protein \u03b1-subunit activated in the initial amplifying step of the phototransduction cascade. Here, we measured human cone outer segment responses in subjects with bradyopsia arising from a triple-deletion mutation in R9AP, the anchor and transport protein for the GTPase-activating protein (GAP) RGS9. Immunoprecipitation showed that the mutant R9AP has greatly reduced affinity for RGS9, predictably reducing the level of RGS9 in the outer segment. The bradyopsia subjects\u2019 elongation responses had normal activation kinetics, amplitude and photosensitivity, but markedly slowed recovery. These results indicate that normal levels of RGS9 are required for deactivation of the cone elongation response, situating the underlying molecular mechanism within the G-protein cascade at or prior to formation of the GAP complex. The recovery of the cone elongation response, measured in a paired-flash paradigm for stimuli isomerizing up to 75% of opsin, revealed that elongation requires a substrate that is depleted and recovers in a bleach-dependent manner. In particular, recovery from the highest bleaching exposure (75%) tracked the time course of cone opsin regeneration, implying that unregenerated cone opsin produces \u201c\n                  <jats:italic>dark light<\/jats:italic>\n                  \u201d, known in rods to arise from constitutive activation of G-protein. Taken together, the results from controls and bradyopsia identify the inactive (GDP-bound) transducin complex as the essential substrate for the human cone elongation response. Suppression of the elongation response in the paired-flash paradigm further revealed the complete profile of the initial fast cone outer segment shrinkage response and reinforced its origin in opsin structural changes. Overall, these results identify key mechanistic elements of the optoretinogram and enable its use as a molecularly interpretable, non- invasive assay of cone function in disease and therapeutic response.\n                <\/jats:p>","DOI":"10.64898\/2026.04.28.721497","type":"posted-content","created":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T22:50:14Z","timestamp":1777675814000},"source":"Crossref","is-referenced-by-count":0,"title":["Optoretinography in R9AP-bradyopsia reveals the essential role of G-protein signaling in the human cone elongation response"],"prefix":"10.64898","author":[{"given":"Connor","family":"Weiss","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, University of Washington School of Medicine"},{"name":"Graduate Program in Neuroscience, University of 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Miami"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9164-7488","authenticated-orcid":false,"given":"Debarshi","family":"Mustafi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University of Washington School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Carroll","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology & Visual Sciences, Medical College of Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirill","family":"Martemyanov","sequence":"additional","affiliation":[{"name":"Department of Physiology & Biophysics, University of Miami"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3293-6538","authenticated-orcid":false,"suffix":"Jr.","given":"Edward N.","family":"Pugh","sequence":"additional","affiliation":[{"name":"Department of Physiology & Membrane Biology, UC 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