{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:29:32Z","timestamp":1785407372213,"version":"3.56.0"},"posted":{"date-parts":[[2026,5,4]]},"group-title":"Developmental Biology","reference-count":139,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["R01HL128503"],"award-info":[{"award-number":["R01HL128503"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01HL171326"],"award-info":[{"award-number":["R01HL171326"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["UM1HG011972"],"award-info":[{"award-number":["UM1HG011972"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["UF1MH128336"],"award-info":[{"award-number":["UF1MH128336"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["U01MH139785"],"award-info":[{"award-number":["U01MH139785"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["P01HL180323"],"award-info":[{"award-number":["P01HL180323"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["916258"],"award-info":[{"award-number":["916258"]}],"id":[{"id":"https:\/\/ror.org\/013kjyp64","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/006w34k90","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/02qenvm24","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Collateral arteries are natural bypasses that can reroute blood flow around arterial blockages, limiting tissue injury during stroke and coronary artery disease. Despite their clinical effectiveness, therapeutic strategies to stimulate collateral artery growth remain unavailable due to our limited understanding of their developmental mechanisms. Remarkably, guinea pigs display exceptionally dense collateral artery networks across various organs, resulting in complete resistance to ischemic damage in the brain and heart. In this study, we compared single-cell RNA sequencing (scRNA-seq) from guinea pig and mouse tissues to identify endothelial cell (EC) gene expression patterns associated with extensive collateral artery development. We then developed an\n                  <jats:italic>in vivo<\/jats:italic>\n                  Perturb-seq platform in mice to test whether genes differentially expressed in guinea pigs influence artery EC specification. This pipeline identified artery repressors that were downregulated in guinea pigs and increased pial collateral abundance when inhibited in mice. Downstream analysis suggests that artery repressors, including WNT and hypoxia response genes, function in two capillary EC subsets\u2014\n                  <jats:italic>Esm1+<\/jats:italic>\n                  pre-artery and\n                  <jats:italic>Apln+<\/jats:italic>\n                  angiogenic tip cells. Reduced activity of these repressors allows more ECs to acquire arterial identity, potentiating collateral artery formation. Collectively, our study establishes a strategy for discovering the genes underlying species-specific traits, suggests that guinea pigs have collaterals due to decreased activity of artery inhibitor pathways and hypoxia responses, and identifies novel targets for stimulating collateral artery formation (\n                  <jats:bold>Graphical abstract<\/jats:bold>\n                  ).\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Abstract Figure<\/jats:title>\n                  <jats:fig id=\"ufig1\" position=\"float\" orientation=\"portrait\" fig-type=\"figure\">\n                    <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"721711v2_ufig1\" position=\"float\" orientation=\"portrait\"\/>\n                  <\/jats:fig>\n                <\/jats:sec>","DOI":"10.64898\/2026.04.29.721711","type":"posted-content","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T09:10:14Z","timestamp":1777885814000},"source":"Crossref","is-referenced-by-count":0,"title":["A Perturb-seq screen guided by species divergence uncovers pathways for collateral artery formation"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4316-0616","authenticated-orcid":false,"given":"Xiaochen","family":"Fan","sequence":"first","affiliation":[{"name":"Department of Biology, Stanford University, Stanford, CA, USA"},{"name":"Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronghao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA"},{"name":"Basic Sciences and Engineering Initiative, Betty Irene Moore Children\u2019s Heart Center, Lucile Packard Children\u2019s Hospital, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian C.","family":"Raftrey","sequence":"additional","affiliation":[{"name":"Department of Biology, Stanford University, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela E.","family":"Rios Coronado","sequence":"additional","affiliation":[{"name":"Department of Biology, Stanford University, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Trimm","sequence":"additional","affiliation":[{"name":"Biophysics Program, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin","family":"Clancy","sequence":"additional","affiliation":[{"name":"Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0408-0813","authenticated-orcid":false,"given":"Xinhong","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie","family":"Bozeman","sequence":"additional","affiliation":[{"name":"Department of Biology, Stanford University, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maggie S.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoxruxxon","family":"Alimukhamedov","sequence":"additional","affiliation":[{"name":"The Phil and Penny Knight Initiative for Brain Resilience, Stanford University, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Alcocer","sequence":"additional","affiliation":[{"name":"Department of Biology, Stanford University, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Idalina","family":"Bonham","sequence":"additional","affiliation":[{"name":"CPP Armamentarium Vector Core, California State Polytechnic University, Pomona, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuti","family":"Agarwal","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Allergy, and Critical Care Medicine, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1113-6889","authenticated-orcid":false,"given":"Alina","family":"Isakova","sequence":"additional","affiliation":[{"name":"The Phil and Penny Knight Initiative for Brain Resilience, Stanford University, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5532-8247","authenticated-orcid":false,"given":"Vinicio A.","family":"de Jesus Perez","sequence":"additional","affiliation":[{"name":"Division of Pulmonary, Allergy, and Critical Care Medicine, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong Y.","family":"Park","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6591-3271","authenticated-orcid":false,"given":"Timothy F.","family":"Shay","sequence":"additional","affiliation":[{"name":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana","family":"Gradinaru","sequence":"additional","affiliation":[{"name":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA"},{"name":"Howard Hughes Medical Institute, Chevy Chase, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7645-9067","authenticated-orcid":false,"given":"Thomas","family":"Quertermous","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5754-1719","authenticated-orcid":false,"given":"Jesse M.","family":"Engreitz","sequence":"additional","affiliation":[{"name":"Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA"},{"name":"Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA"},{"name":"Basic Sciences and Engineering Initiative, Betty Irene Moore Children\u2019s Heart Center, Lucile Packard Children\u2019s Hospital, Stanford, California, USA"},{"name":"The Gene Regulation Observatory, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA"},{"name":"The Novo Nordisk Foundation Center for Genomic Mechanisms of Disease, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristy","family":"Red-Horse","sequence":"additional","affiliation":[{"name":"Department of Biology, Stanford University, Stanford, CA, USA"},{"name":"Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA"},{"name":"Howard Hughes Medical Institute, Chevy Chase, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026052521150639000_2026.04.29.721711v2.1","doi-asserted-by":"publisher","DOI":"10.1161\/cir.0000000000000757"},{"key":"2026052521150639000_2026.04.29.721711v2.2","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.9349"},{"key":"2026052521150639000_2026.04.29.721711v2.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2010.03.014"},{"key":"2026052521150639000_2026.04.29.721711v2.4","doi-asserted-by":"publisher","DOI":"10.1007\/s11886-021-01460-z"},{"key":"2026052521150639000_2026.04.29.721711v2.5","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X251322378"},{"key":"2026052521150639000_2026.04.29.721711v2.6","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.31.1.128"},{"key":"2026052521150639000_2026.04.29.721711v2.7","doi-asserted-by":"publisher","DOI":"10.4414\/smw.2017.14538"},{"key":"2026052521150639000_2026.04.29.721711v2.8","doi-asserted-by":"publisher","DOI":"10.1186\/s12883-016-0722-0"},{"key":"2026052521150639000_2026.04.29.721711v2.9","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/eht195"},{"key":"2026052521150639000_2026.04.29.721711v2.10","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2016-011105"},{"key":"2026052521150639000_2026.04.29.721711v2.11","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5273(84)90326-7"},{"key":"2026052521150639000_2026.04.29.721711v2.12","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/21.10.737"},{"key":"2026052521150639000_2026.04.29.721711v2.13","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X20942609"},{"key":"2026052521150639000_2026.04.29.721711v2.14","doi-asserted-by":"publisher","DOI":"10.1007\/BF01935807"},{"key":"2026052521150639000_2026.04.29.721711v2.15","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00047.2007"},{"key":"2026052521150639000_2026.04.29.721711v2.16","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X231194956"},{"key":"2026052521150639000_2026.04.29.721711v2.17","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.114.303929"},{"key":"2026052521150639000_2026.04.29.721711v2.18","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.778773"},{"key":"2026052521150639000_2026.04.29.721711v2.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2025.103611"},{"key":"2026052521150639000_2026.04.29.721711v2.20","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvw005"},{"key":"2026052521150639000_2026.04.29.721711v2.21","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.12.023"},{"key":"2026052521150639000_2026.04.29.721711v2.22","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2024.114771"},{"key":"2026052521150639000_2026.04.29.721711v2.23","doi-asserted-by":"publisher","DOI":"10.1111\/j.1549-8719.2010.00034.x"},{"key":"2026052521150639000_2026.04.29.721711v2.24","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2022.01.005"},{"key":"2026052521150639000_2026.04.29.721711v2.25","doi-asserted-by":"publisher","DOI":"10.1101\/2023.10.09.561542"},{"key":"2026052521150639000_2026.04.29.721711v2.26","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20153608"},{"key":"2026052521150639000_2026.04.29.721711v2.27","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2024.07.005"},{"key":"2026052521150639000_2026.04.29.721711v2.28","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.181115"},{"key":"2026052521150639000_2026.04.29.721711v2.29","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.112.279109"},{"key":"2026052521150639000_2026.04.29.721711v2.30","doi-asserted-by":"publisher","DOI":"10.1242\/dev.092304"},{"key":"2026052521150639000_2026.04.29.721711v2.31","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.116.014160"},{"key":"2026052521150639000_2026.04.29.721711v2.32","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X20924107"},{"key":"2026052521150639000_2026.04.29.721711v2.33","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2020.09.015"},{"key":"2026052521150639000_2026.04.29.721711v2.34","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.123.319319"},{"key":"2026052521150639000_2026.04.29.721711v2.35","doi-asserted-by":"publisher","DOI":"10.64898\/2026.02.23.707374"},{"key":"2026052521150639000_2026.04.29.721711v2.36","doi-asserted-by":"publisher","DOI":"10.1126\/science.aax4438"},{"key":"2026052521150639000_2026.04.29.721711v2.37","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.05.013"},{"key":"2026052521150639000_2026.04.29.721711v2.38","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaz6063"},{"key":"2026052521150639000_2026.04.29.721711v2.39","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.04.050"},{"key":"2026052521150639000_2026.04.29.721711v2.40","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2008.11.023"},{"key":"2026052521150639000_2026.04.29.721711v2.41","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.10036"},{"key":"2026052521150639000_2026.04.29.721711v2.42","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0288-7"},{"key":"2026052521150639000_2026.04.29.721711v2.43","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.01.015"},{"key":"2026052521150639000_2026.04.29.721711v2.44","doi-asserted-by":"publisher","DOI":"10.1038\/nature25739"},{"key":"2026052521150639000_2026.04.29.721711v2.45","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07493-y"},{"key":"2026052521150639000_2026.04.29.721711v2.46","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.120.318271"},{"key":"2026052521150639000_2026.04.29.721711v2.47","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.70246"},{"key":"2026052521150639000_2026.04.29.721711v2.48","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21246-9"},{"key":"2026052521150639000_2026.04.29.721711v2.49","doi-asserted-by":"publisher","DOI":"10.1101\/gad.301549.117"},{"key":"2026052521150639000_2026.04.29.721711v2.50","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.302953"},{"key":"2026052521150639000_2026.04.29.721711v2.51","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvae201"},{"key":"2026052521150639000_2026.04.29.721711v2.52","doi-asserted-by":"publisher","DOI":"10.1101\/gr.271205.120"},{"key":"2026052521150639000_2026.04.29.721711v2.53","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.06.037"},{"key":"2026052521150639000_2026.04.29.721711v2.54","doi-asserted-by":"publisher","DOI":"10.1038\/nature14362"},{"key":"2026052521150639000_2026.04.29.721711v2.55","doi-asserted-by":"publisher","DOI":"10.1038\/nature16498"},{"key":"2026052521150639000_2026.04.29.721711v2.56","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-021-00637-6"},{"key":"2026052521150639000_2026.04.29.721711v2.57","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-38582-7"},{"key":"2026052521150639000_2026.04.29.721711v2.58","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-021-01207-2"},{"key":"2026052521150639000_2026.04.29.721711v2.59","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.0106"},{"key":"2026052521150639000_2026.04.29.721711v2.60","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0470-y"},{"key":"2026052521150639000_2026.04.29.721711v2.61","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-023-00684-y"},{"key":"2026052521150639000_2026.04.29.721711v2.62","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.124.324868"},{"key":"2026052521150639000_2026.04.29.721711v2.63","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.09.004"},{"key":"2026052521150639000_2026.04.29.721711v2.64","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3555"},{"key":"2026052521150639000_2026.04.29.721711v2.65","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6758"},{"key":"2026052521150639000_2026.04.29.721711v2.66","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-46300-0"},{"key":"2026052521150639000_2026.04.29.721711v2.67","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-64047-0"},{"key":"2026052521150639000_2026.04.29.721711v2.68","doi-asserted-by":"publisher","DOI":"10.1038\/nature05571"},{"key":"2026052521150639000_2026.04.29.721711v2.69","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200302047"},{"key":"2026052521150639000_2026.04.29.721711v2.70","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.01.015"},{"key":"2026052521150639000_2026.04.29.721711v2.71","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-02-270819"},{"key":"2026052521150639000_2026.04.29.721711v2.72","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-07-230284"},{"key":"2026052521150639000_2026.04.29.721711v2.73","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-05-424713"},{"key":"2026052521150639000_2026.04.29.721711v2.74","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-021-02545-2"},{"key":"2026052521150639000_2026.04.29.721711v2.75","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1589207"},{"key":"2026052521150639000_2026.04.29.721711v2.76","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-3018-x"},{"key":"2026052521150639000_2026.04.29.721711v2.77","doi-asserted-by":"publisher","DOI":"10.1038\/nature05577"},{"key":"2026052521150639000_2026.04.29.721711v2.78","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.19.3675"},{"key":"2026052521150639000_2026.04.29.721711v2.79","doi-asserted-by":"publisher","DOI":"10.1242\/dev.150904"},{"key":"2026052521150639000_2026.04.29.721711v2.80","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.05.024"},{"key":"2026052521150639000_2026.04.29.721711v2.81","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2022.10.007"},{"key":"2026052521150639000_2026.04.29.721711v2.82","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01742-7"},{"key":"2026052521150639000_2026.04.29.721711v2.83","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33324-7"},{"key":"2026052521150639000_2026.04.29.721711v2.84","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-021-01346-6"},{"key":"2026052521150639000_2026.04.29.721711v2.85","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.43803"},{"key":"2026052521150639000_2026.04.29.721711v2.86","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.07.047"},{"key":"2026052521150639000_2026.04.29.721711v2.87","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.10.042"},{"key":"2026052521150639000_2026.04.29.721711v2.88","doi-asserted-by":"publisher","DOI":"10.1126\/science.1059817"},{"key":"2026052521150639000_2026.04.29.721711v2.89","doi-asserted-by":"publisher","DOI":"10.1126\/science.1059796"},{"key":"2026052521150639000_2026.04.29.721711v2.90","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2008.04.009"},{"key":"2026052521150639000_2026.04.29.721711v2.91","doi-asserted-by":"publisher","DOI":"10.1159\/000080345"},{"key":"2026052521150639000_2026.04.29.721711v2.92","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-23-1143"},{"key":"2026052521150639000_2026.04.29.721711v2.93","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.11.5667"},{"key":"2026052521150639000_2026.04.29.721711v2.94","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.51.32529"},{"key":"2026052521150639000_2026.04.29.721711v2.95","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002443107"},{"key":"2026052521150639000_2026.04.29.721711v2.96","doi-asserted-by":"publisher","DOI":"10.1126\/science.1189406"},{"key":"2026052521150639000_2026.04.29.721711v2.97","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3067"},{"key":"2026052521150639000_2026.04.29.721711v2.98","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.9.4604"},{"key":"2026052521150639000_2026.04.29.721711v2.99","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.00471"},{"key":"2026052521150639000_2026.04.29.721711v2.100","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2016.06.018"},{"key":"2026052521150639000_2026.04.29.721711v2.101","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00285"},{"key":"2026052521150639000_2026.04.29.721711v2.102","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012389"},{"key":"2026052521150639000_2026.04.29.721711v2.103","doi-asserted-by":"publisher","DOI":"10.1007\/bf00584425"},{"key":"2026052521150639000_2026.04.29.721711v2.104","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-4584-1_44"},{"key":"2026052521150639000_2026.04.29.721711v2.105","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3574"},{"key":"2026052521150639000_2026.04.29.721711v2.106","doi-asserted-by":"publisher","DOI":"10.64898\/2026.02.06.704033"},{"key":"2026052521150639000_2026.04.29.721711v2.107","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2022.09.002"},{"key":"2026052521150639000_2026.04.29.721711v2.108","doi-asserted-by":"publisher","DOI":"10.1101\/2024.01.23.576920"},{"key":"2026052521150639000_2026.04.29.721711v2.109","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.10.010"},{"key":"2026052521150639000_2026.04.29.721711v2.110","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0000000000000655"},{"key":"2026052521150639000_2026.04.29.721711v2.111","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"2026052521150639000_2026.04.29.721711v2.112","doi-asserted-by":"publisher","DOI":"10.1038\/s44161-022-00114-9"},{"key":"2026052521150639000_2026.04.29.721711v2.113","doi-asserted-by":"publisher","DOI":"10.17504\/protocols.io.6t8herw"},{"key":"2026052521150639000_2026.04.29.721711v2.114","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-43458-x"},{"key":"2026052521150639000_2026.04.29.721711v2.115","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"2026052521150639000_2026.04.29.721711v2.116","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad1059"},{"key":"2026052521150639000_2026.04.29.721711v2.117","doi-asserted-by":"publisher","DOI":"10.1101\/2020.10.12.335331"},{"key":"2026052521150639000_2026.04.29.721711v2.118","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.73600.2"},{"key":"2026052521150639000_2026.04.29.721711v2.119","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.97"},{"key":"2026052521150639000_2026.04.29.721711v2.120","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad0501"},{"key":"2026052521150639000_2026.04.29.721711v2.121","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2021.06.043"},{"key":"2026052521150639000_2026.04.29.721711v2.122","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-017-1382-0"},{"key":"2026052521150639000_2026.04.29.721711v2.123","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2015.12.004"},{"key":"2026052521150639000_2026.04.29.721711v2.124","doi-asserted-by":"publisher","DOI":"10.1093\/bioadv\/vbac016"},{"key":"2026052521150639000_2026.04.29.721711v2.125","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-021-01282-5"},{"key":"2026052521150639000_2026.04.29.721711v2.126","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau0730"},{"key":"2026052521150639000_2026.04.29.721711v2.127","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0969-x"},{"key":"2026052521150639000_2026.04.29.721711v2.128","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05688-9"},{"key":"2026052521150639000_2026.04.29.721711v2.129","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr539"},{"key":"2026052521150639000_2026.04.29.721711v2.130","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq636"},{"key":"2026052521150639000_2026.04.29.721711v2.131","doi-asserted-by":"publisher","DOI":"10.7554\/elife.19760"},{"key":"2026052521150639000_2026.04.29.721711v2.132","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3437"},{"key":"2026052521150639000_2026.04.29.721711v2.133","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15178"},{"key":"2026052521150639000_2026.04.29.721711v2.134","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1000"},{"key":"2026052521150639000_2026.04.29.721711v2.135","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"2026052521150639000_2026.04.29.721711v2.136","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.27"},{"key":"2026052521150639000_2026.04.29.721711v2.137","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-024-02303-9"},{"key":"2026052521150639000_2026.04.29.721711v2.138","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-021-02584-9"},{"key":"2026052521150639000_2026.04.29.721711v2.139","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-025-02793-1"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.04.29.721711","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T04:15:19Z","timestamp":1779768919000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.04.29.721711"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,4]]},"references-count":139,"URL":"https:\/\/doi.org\/10.64898\/2026.04.29.721711","relation":{},"subject":[],"published":{"date-parts":[[2026,5,4]]},"subtype":"preprint"}}