{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:21:19Z","timestamp":1785432079697,"version":"3.56.0"},"posted":{"date-parts":[[2026,5,4]]},"group-title":"Bioinformatics","reference-count":85,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["DK077195"],"award-info":[{"award-number":["DK077195"]}],"id":[{"id":"https:\/\/ror.org\/00adh9b73","id-type":"ROR","asserted-by":"publisher"}]},{"award":["DK127673"],"award-info":[{"award-number":["DK127673"]}],"id":[{"id":"https:\/\/ror.org\/00adh9b73","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Bladder outlet obstruction leads to pathological remodeling and emergence of lower urinary tract symptoms. Although relief of obstruction is associated with symptomatic improvement, it is not universally successful, reflecting persistent alterations in the bladder. Reliable surrogate biomarkers of obstruction are lacking, particularly early in the disease course before irreversible damage to the bladder may have occurred. In this study, re-analysis of publicly available transcriptomic datasets from diverse rodent models of obstruction identified tissue transcripts including\n                  <jats:italic>Cthrc1<\/jats:italic>\n                  ,\n                  <jats:italic>Grem1<\/jats:italic>\n                  ,\n                  <jats:italic>Ltbp2<\/jats:italic>\n                  and\n                  <jats:italic>Msn<\/jats:italic>\n                  that were induced in response to injury. Candidate markers were validated experimentally in an independent model of neurogenic obstruction demonstrating time-dependent changes. Candidate markers were also attenuated with either surgical removal of obstruction or treatment with anticholinergic medication or inosine. Integrated analysis of tissue transcriptomics data and tissue and urine proteomics data from a model of neurogenic obstruction revealed significant concordance between markers observed in tissue and urine. Urinary proteomics analysis identified a statistically significant increase in MSN in patients with neurogenic bladder compared to unaffected controls. These findings identify tissue and urine biomarkers of both non-neurogenic and neurogenic obstruction that may reflect early changes in obstructive uropathy that could be monitored in a non-invasive manner.\n                <\/jats:p>","DOI":"10.64898\/2026.04.29.721732","type":"posted-content","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T17:40:17Z","timestamp":1777916417000},"source":"Crossref","is-referenced-by-count":0,"title":["Integrated transcriptomic and proteomic analyses identify novel biomarkers of bladder outlet obstruction"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6914-9135","authenticated-orcid":false,"given":"Alexander A.","family":"Bigger-Allen","sequence":"first","affiliation":[{"name":"Urological Diseases Research Center, Boston Children\u2019s Hospital, Boston, MA, USA"},{"name":"Department of Surgery, Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barnali","family":"Das","sequence":"additional","affiliation":[{"name":"Urological Diseases Research Center, Boston Children\u2019s Hospital, Boston, MA, USA"},{"name":"Department of Surgery, Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Tang","sequence":"additional","affiliation":[{"name":"Urological Diseases Research Center, Boston Children\u2019s Hospital, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle","family":"Costa","sequence":"additional","affiliation":[{"name":"Urological Diseases Research Center, Boston Children\u2019s Hospital, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Luis","family":"Ocampo","sequence":"additional","affiliation":[{"name":"Urological Diseases Research Center, Boston Children\u2019s Hospital, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9625-6259","authenticated-orcid":false,"given":"Ali Hashemi","family":"Gheinani","sequence":"additional","affiliation":[{"name":"Functional Urology Research Group, Department for BioMedical Research DBMR, University of Bern, Switzerland"},{"name":"Department of Urology, Inselspital University Hospital, 3010 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon","family":"DiMartino","sequence":"additional","affiliation":[{"name":"Urological Diseases Research Center, Boston Children\u2019s Hospital, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane","family":"Kaull","sequence":"additional","affiliation":[{"name":"Urological Diseases Research Center, Boston Children\u2019s Hospital, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Froehlich","sequence":"additional","affiliation":[{"name":"Urological Diseases Research Center, Boston Children\u2019s Hospital, Boston, MA, USA"},{"name":"Department of Surgery, Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Lee","sequence":"additional","affiliation":[{"name":"Urological Diseases Research Center, Boston Children\u2019s Hospital, Boston, MA, USA"},{"name":"Department of Surgery, Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0943-6236","authenticated-orcid":false,"given":"Rosalyn M.","family":"Adam","sequence":"additional","affiliation":[{"name":"Urological Diseases Research Center, Boston Children\u2019s Hospital, Boston, MA, USA"},{"name":"Department of Surgery, Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026050704151029000_2026.04.29.721732v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2006.07.050"},{"key":"2026050704151029000_2026.04.29.721732v1.2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s11934-024-01202-y","article-title":"Persistent Lower Urinary Tract Symptoms After BPH Surgery","volume":"25","year":"2024","journal-title":"Curr Urol Rep"},{"key":"2026050704151029000_2026.04.29.721732v1.3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2010.09445.x"},{"issue":"Suppl 5","key":"2026050704151029000_2026.04.29.721732v1.4","first-page":"S56","article-title":"Do functional changes occur in the bladder due to bladder outlet obstruction? 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