{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T19:13:28Z","timestamp":1778094808648,"version":"3.51.4"},"posted":{"date-parts":[[2026,5,4]]},"group-title":"Surgery","reference-count":32,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T00:00:00Z","timestamp":1777852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Phenome-wide association studies (PheWAS) can reveal novel associations between variants in drug-target genes and disease and, as such, can be used to predict new drug-indication pairs for repurposing drugs with a known mechanism of action. A platelet-derived growth factor receptor beta (PDGFR\u03b2) PheWAS demonstrated that patients with a single nucleotide variant that reduces PDGFR\u03b2 expression exhibit a higher prevalence of chronic skin ulcers, skin grafts, and reconstructive surgeries. Recombinant human platelet derived growth factor BB (rhPDGF) is a therapeutic that binds to and activates PDGFR\u03b2 and has received FDA approval for multiple indications, including improving healing of lower extremity diabetic neuropathic ulcers, augmenting periodontal bone and soft tissue reconstruction, and stimulating orthopedic bone regeneration. Leveraging a drug-repurposing methodology informed by PheWAS, we hypothesize that rhPDGF will provide therapeutic benefit in the treatment of other complex wounds, like full-thickness surgical wounds of the head or neck that cannot heal by primary intention following skin cancer excision.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>\n                    This prospective, double-blinded, single-site study aims to enroll 40 participants, randomized at a ratio of 1:1, comparing the efficacy of an advanced wound matrix saturated with rhPDGF or saline. Comparisons will be stratified by anatomical location (scalp\/forehead versus face\/neck) and maximum surgical defect dimensions (\n                    <jats:underline>&lt;<\/jats:underline>\n                    3cm versus &gt; 3cm). The primary outcome of this study will evaluate the time in days to 81-100% granulation of the wound bed by expert clinical assessment of daily photographs. Secondary outcomes will assess the superiority of the rhPDGF-enhanced wound matrix relative to control with respect to wound granulation rate, epithelialization, complete wound healing, and patient reported outcomes (PROMs).\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Discussion<\/jats:title>\n                  <jats:p>Although reconstructive techniques are available for healing complex head and neck wounds following skin cancer excision, these procedures are invasive, and older, frail patients are often suboptimal candidates. There remains a need for less invasive therapeutic approaches that reduce the healing time and mitigate the morbidity associated with chronic wounds. A PheWAS analysis identified complex wounds requiring reconstructive surgery as a novel drug-indication pair for repurposing rhPDGF. This protocol is designed to evaluate the efficacy of an rhPDGF-enhanced advanced wound matrix for healing complex head and neck wounds post skin cancer excision that cannot heal by primary intention.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Clinical trial registration<\/jats:title>\n                  <jats:p>\n                    This trial is registered at ClinicalTrials.gov (\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"clintrialgov\" xlink:href=\"NCT06634030\">NCT06634030<\/jats:ext-link>\n                    ).\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.05.01.26352276","type":"posted-content","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T22:40:14Z","timestamp":1777934414000},"source":"Crossref","is-referenced-by-count":0,"title":["Study protocol and statistical analysis plan for a randomized, double-blind, controlled trial evaluating recombinant human platelet-derived growth factor B (rhPDGF) in the reconstruction of complex head or neck defects following skin cancer excision"],"prefix":"10.64898","author":[{"given":"Cyndi R","family":"Clark","sequence":"first","affiliation":[{"name":"Vanderbilt Institute of Clinical and Translational Research, Vanderbilt Health, Nashville, Tennessee, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3121-7718","authenticated-orcid":false,"given":"Bryan S","family":"Blette","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Vanderbilt Health, Nashville, Tennessee, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4546-551X","authenticated-orcid":false,"given":"Ricardo A Torres","family":"Guzman","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, Vanderbilt Health, Nashville, Tennessee, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5784-4517","authenticated-orcid":false,"given":"Melissa D","family":"Lempicki","sequence":"additional","affiliation":[{"name":"Vanderbilt Institute of Clinical and Translational Research, Vanderbilt Health, Nashville, Tennessee, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2208-8539","authenticated-orcid":false,"given":"Georgios","family":"Karamitros","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, Vanderbilt Health, Nashville, Tennessee, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franklin","family":"Gergoudis","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, Vanderbilt Health, Nashville, Tennessee, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barite W","family":"Gutama","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, Vanderbilt Health, Nashville, Tennessee, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel R","family":"O\u2019Neill","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, Vanderbilt Health, Nashville, Tennessee, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"Savitz","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery, Vanderbilt Health, Nashville, Tennessee, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob","family":"Smith","sequence":"additional","affiliation":[{"name":"Vanderbilt Institute of Clinical and Translational Research, Vanderbilt Health, Nashville, Tennessee, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana K","family":"Shirey-Rice","sequence":"additional","affiliation":[{"name":"Vanderbilt Institute of Clinical and Translational Research, Vanderbilt Health, Nashville, Tennessee, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jill M","family":"Pulley","sequence":"additional","affiliation":[{"name":"Vanderbilt Institute of Clinical and Translational Research, Vanderbilt Health, Nashville, Tennessee, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel E","family":"Lynch","sequence":"additional","affiliation":[{"name":"Lynch Regenerative Medicine, Franklin, Tennessee, United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7091-9608","authenticated-orcid":false,"given":"Trey W","family":"McGonigle","sequence":"additional","affiliation":[{"name":"Department of 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