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Transmembrane components of the PCP complex bridge cell junctions and organize into punctate, intercellular assemblies that exhibit a high degree of stability. Here, we define the contributions of the cytoplasmic PCP protein, Dishevelled (Dvl), in the sub-micron scale organization and stability of PCP complexes. Using endogenously-tagged fluorescent PCP reporters in the embryonic mouse epidermis, we quantify PCP protein mobility and clustering during polarization. We find that as transmembrane proteins immobilize into puncta, Dishevelled (Dvl2\/3) co-accumulates with its transmembrane partner Frizzled (Fz6) in a polarized manner and stabilizes clusters of PCP complexes. We identify a previously unknown function for the oligomerizing DIX domain of Dvl3, typically associated with Wnt signaling, in Dvl3 asymmetric localization. These observations underscore a role for Dvl oligomerization in assembly and stabilization of asymmetric PCP puncta.<\/jats:p>","DOI":"10.64898\/2026.05.01.722170","type":"posted-content","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:35:21Z","timestamp":1778027721000},"source":"Crossref","is-referenced-by-count":0,"title":["Dishevelled-mediated clustering stabilizes Frizzled6 and Vangl2 to establish planar cell polarity in the mammalian skin"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8391-8110","authenticated-orcid":false,"given":"Parijat","family":"Sil","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Princeton University, Princeton, NJ 08544"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brandon","family":"Trejo","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Princeton University, Princeton, NJ 08544"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine A.","family":"Little","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Princeton University, Princeton, NJ 08544"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danelle","family":"Devenport","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Princeton University, Princeton, NJ 08544"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"54368","reference":[{"key":"2026050812100634000_2026.05.01.722170v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.11"},{"key":"2026050812100634000_2026.05.01.722170v1.2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.054080"},{"key":"2026050812100634000_2026.05.01.722170v1.3","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201408039"},{"key":"2026050812100634000_2026.05.01.722170v1.4","doi-asserted-by":"publisher","DOI":"10.1042\/bst20190404"},{"key":"2026050812100634000_2026.05.01.722170v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1784"},{"key":"2026050812100634000_2026.05.01.722170v1.6","doi-asserted-by":"crossref","unstructured":"L. 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Devenport , Celsr1 adhesive interactions mediate the asymmetric organization of planar polarity complexes. eLife 10, (2021).","DOI":"10.7554\/eLife.62097"},{"key":"2026050812100634000_2026.05.01.722170v1.9","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00715-8"},{"key":"2026050812100634000_2026.05.01.722170v1.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.03.018"},{"key":"2026050812100634000_2026.05.01.722170v1.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.08.016"},{"key":"2026050812100634000_2026.05.01.722170v1.12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005259"},{"key":"2026050812100634000_2026.05.01.722170v1.13","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1016\/j.celrep.2018.10.039","article-title":"Rapid Disruption of Dishevelled Activity Uncovers an Intercellular Role in Maintenance of Prickle in Core Planar Polarity Protein Complexes","volume":"25","year":"2018","journal-title":"Cell reports"},{"key":"2026050812100634000_2026.05.01.722170v1.14","doi-asserted-by":"crossref","first-page":"9503","DOI":"10.1038\/s41467-025-64563-z","article-title":"Cell autonomous polarization by the planar cell polarity signaling pathway","volume":"16","year":"2025","journal-title":"Nat Commun"},{"key":"2026050812100634000_2026.05.01.722170v1.15","doi-asserted-by":"crossref","unstructured":"S. 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