{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:29:56Z","timestamp":1785407396583,"version":"3.56.0"},"posted":{"date-parts":[[2026,5,7]]},"group-title":"Genomics","reference-count":30,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T00:00:00Z","timestamp":1778112000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"award":["NIH T32 AR069512"],"award-info":[{"award-number":["NIH T32 AR069512"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["P30 AR070549"],"award-info":[{"award-number":["P30 AR070549"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018208","name":"Cure JM Foundation","doi-asserted-by":"crossref","award":["Fellowship Award"],"award-info":[{"award-number":["Fellowship Award"]}],"id":[{"id":"10.13039\/100018208","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Cincinnati Children's Research Foundation","award":["ARC Award #53632"],"award-info":[{"award-number":["ARC Award #53632"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Juvenile dermatomyositis (JDM) is a chronic multisystem vasculopathy and inflammatory myopathy characterized by proximal muscle weakness, distinct rash, and risk of complications such as calcinosis cutis, skin ulceration, and mortality. Molecular insight from diagnostic muscle biopsy histology is limited, and the mechanistic pathoetiology of JDM remains poorly defined. We used single nuclei transcriptomics to assess muscle samples from patients with newly diagnosed treatment-na\u00efve JDM. As a control, we assessed muscle samples from patients with congenital (nemaline) myopathy (CM), a non-inflammatory disorder. A total of 25,794 high quality nuclei were analyzed and clustered into various muscle-resident or infiltrating cellular populations. JDM tissue was characterized by an enriched interferon (IFN) response signature across endothelial, stromal, and immune cell compartments. Endothelial and perivascular populations showed increased inflammatory and angiogenic programs. Intercellular communication inference analysis identified dysregulated vascular endothelial growth factor (VEGF)-related signaling involving endothelial, stromal, and myonuclear populations as a possible mechanism for myonuclear-driven modulation of the muscle microvasculature. Spatial RNA in situ hybridization supported increased expression of selected IFN responsive and angiogenesis signaling genes in JDM tissue. Collectively, these data provide a cell type-resolved view of treatment-na\u00efve JDM muscle and highlight vascular and IFN pathways for follow-up in larger cohorts.<\/jats:p>","DOI":"10.64898\/2026.05.04.721749","type":"posted-content","created":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T22:00:13Z","timestamp":1778191213000},"source":"Crossref","is-referenced-by-count":0,"title":["Single nucleus RNA sequencing of juvenile dermatomyositis skeletal muscle identifies altered angiogenic signaling"],"prefix":"10.64898","author":[{"given":"Casey O.","family":"Swoboda","sequence":"first","affiliation":[{"name":"Cincinnati Children\u2019s Hospital Medical Center (CCHMC), Division of Biomedical Informatics, Cincinnati, OH, United States;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6418-5794","authenticated-orcid":false,"given":"Carmy","family":"Forney","sequence":"additional","affiliation":[{"name":"Cincinnati Children\u2019s 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