{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:55:30Z","timestamp":1785412530423,"version":"3.56.0"},"posted":{"date-parts":[[2026,5,7]]},"group-title":"Genetics","reference-count":77,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T00:00:00Z","timestamp":1778112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fairbairn Foundation HMS Lyme Initiative"},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R21AI168592"],"award-info":[{"award-number":["R21AI168592"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R01AI186279"],"award-info":[{"award-number":["R01AI186279"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R01AI116523"],"award-info":[{"award-number":["R01AI116523"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["P01AI138949"],"award-info":[{"award-number":["P01AI138949"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/006w34k90","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The black legged tick,\n                  <jats:italic>Ixodes scapularis<\/jats:italic>\n                  , is a vector of the bacterium that causes Lyme disease and several other illnesses, including anaplasmosis, babesiosis, and tick-borne encephalitis. Although high-quality genome annotations are available for\n                  <jats:italic>I. scapularis<\/jats:italic>\n                  , functional understanding of\n                  <jats:italic>I. scapularis<\/jats:italic>\n                  genes is limited. To address this, we developed a platform for genome-wide CRISPR-Cas9 knockout screening in\n                  <jats:italic>I. scapularis<\/jats:italic>\n                  cells. To evaluate the platform, we performed a screen to identify genes associated with cellular fitness, and screens for resistance to treatment with copper chloride, Antimycin A, or Destruxin A (DA), a cyclic hexadepsipeptide produced by the pathogenic fungus\n                  <jats:italic>Metarhizium anisopliae<\/jats:italic>\n                  . In each case, the screens implicate specific sets of conserved and non-conserved\n                  <jats:italic>I. scapularis<\/jats:italic>\n                  genes in relevant cellular functions, providing the first experimental evidence of function for a large set of\n                  <jats:italic>I. scapularis<\/jats:italic>\n                  genes. Altogether, in this first-of-its-kind effort for the arthropod subclass Acari, we present an unbiased genome-wide CRISPR-Cas9 knockout cell screening platform, related resources, and datasets that will be broadly useful to efficiently uncover cellular functions of\n                  <jats:italic>I. scapularis<\/jats:italic>\n                  genes.\n                <\/jats:p>","DOI":"10.64898\/2026.05.05.721418","type":"posted-content","created":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T04:55:13Z","timestamp":1778216113000},"source":"Crossref","is-referenced-by-count":0,"title":["Genome-wide CRISPR knockout cell screening platform for the disease vector tick species\n                  <i>Ixodes scapularis<\/i>"],"prefix":"10.64898","author":[{"given":"Matthew","family":"Butnaru","sequence":"first","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston, MA 02115"},{"name":"Howard Hughes Medical Institute, Boston, MA 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