{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T15:15:52Z","timestamp":1778512552620,"version":"3.51.4"},"posted":{"date-parts":[[2026,5,6]]},"group-title":"Microbiology","reference-count":99,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T00:00:00Z","timestamp":1778025600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  <jats:italic>Mycobacterium abscessus<\/jats:italic>\n                  (\n                  <jats:italic>Mab<\/jats:italic>\n                  ) is an opportunistic pathogen that can cause chronic, debilitating lung disease.\n                  <jats:italic>Mab<\/jats:italic>\n                  is intrinsically resistant to most antibiotics, making\n                  <jats:italic>Mab<\/jats:italic>\n                  infections challenging to manage and frequently incurable. During infection,\n                  <jats:italic>Mab<\/jats:italic>\n                  adapts to survive various stresses, including hypoxia and nutrient starvation.\n                  <jats:italic>In vitro<\/jats:italic>\n                  , these conditions drive\n                  <jats:italic>Mab<\/jats:italic>\n                  into a drug-tolerant, non-replicating state. Changes in the\n                  <jats:italic>Mab<\/jats:italic>\n                  proteome that result from entering a non-replicating state have been minimally described despite the clinical importance of this physiological state. Using\n                  <jats:italic>Mab<\/jats:italic>\n                  reference strain ATCC 19977, we collected proteomic data comparing replicating to non-replicating states using a carbon starvation (CS) model of persistence. We identified 2251 proteins overall (46% proteome coverage), and 17% of these proteins were found in only one of the two conditions. A third of identified proteins were significantly changed in abundance, indicating an extensive proteomic response to CS. The response regulator DosR and many DosRS responsive proteins were significantly more abundant under CS, suggesting that the DosRS stress response regulator plays a key role in CS-induced\n                  <jats:italic>Mab<\/jats:italic>\n                  persistence. Many aspects of cell wall biosynthesis were changed, including changes in glycolipid abundance under CS. Proteins involved in other key cellular processes such as secretion, oxidative phosphorylation, and nutrient metabolism were altered under CS. The proteomic analysis presented provides new insights and clarity into how the\n                  <jats:italic>Mab<\/jats:italic>\n                  proteome is regulated during non-replicating persistence, a key consideration for understanding\n                  <jats:italic>Mab<\/jats:italic>\n                  pathophysiology.\n                <\/jats:p>","DOI":"10.64898\/2026.05.05.723019","type":"posted-content","created":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T07:50:16Z","timestamp":1778140216000},"source":"Crossref","is-referenced-by-count":0,"title":["Carbon starvation of\n                  <i>Mycobacterium abscessus<\/i>\n                  induces a non-replicating state with extensive proteomic remodeling"],"prefix":"10.64898","author":[{"given":"Kaylyn L.","family":"Devlin","sequence":"first","affiliation":[{"name":"Department of Chemical Physiology and Biochemistry, Oregon Health & Science University, Portland, Oregon 97239"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gyanu","family":"Lamichhane","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, School of Medicine, Johns Hopkins University, Baltimore, Maryland 21231"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1873-3929","authenticated-orcid":false,"given":"William C.","family":"Nelson","sequence":"additional","affiliation":[{"name":"Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9159-0031","authenticated-orcid":false,"given":"Vivian S.","family":"Lin","sequence":"additional","affiliation":[{"name":"Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354"},{"name":"Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2303-8779","authenticated-orcid":false,"given":"Kimberly E.","family":"Beatty","sequence":"additional","affiliation":[{"name":"Department of Chemical Physiology and Biochemistry, Oregon Health & Science University, Portland, Oregon 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