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However, regulatory mechanisms fine-tuning B-T cell interactions within the GC to prevent aberrant activation and proliferation remain incompletely understood. Here, we identify Siglec-G, the mouse ortholog of human Siglec-10, as an immune checkpoint that restrains the GC by dampening B-T cell interactions. Selective and temporal ablation of Siglec-G on B cells after immunization triggers GC hyperplasia and enhanced plasma cell and antibody output. While Siglec-G is dispensable in B cell receptor (BCR)-mediated processes, it acts as an intrinsic inhibitory receptor of B-T cell interactions in the GC, ultimately limiting Myc and mTORC activation within positively selected GC B cells.\n                  <jats:italic>Trans<\/jats:italic>\n                  interactions of Siglec-G and its glycan ligands on Tfh likely contribute in fine-tuning the strength of bidirectional signaling following contact between GC B cells and Tfh cells. This interaction is further reinforced by glycan remodeling that occurs in the GC, resulting in concurrent decreased in glycan ligands on GC B cells and increased in glycan ligands on Tfh. This augmented binding of Siglec-G\/10 on Tfh is mainly due to the upregulation of \u03b12-6 linked sialic acid ligands. Moreover, APEX2-based proximity labeling revealed several candidate Siglec-G\/10 binding partners on T cells, including BTLA, CD6, and Slamf6, which are known negative regulators of Tfh cell activation. Taken together, our findings identified that Siglec-G acts as a GC checkpoint receptor, restricting B cell proliferation by tuning T cell help following B-T cell interactions.\n                <\/jats:p>","DOI":"10.64898\/2026.05.06.723371","type":"posted-content","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T13:35:17Z","timestamp":1778506517000},"source":"Crossref","is-referenced-by-count":0,"title":["Siglec-G on B cells restrains the germinal center response by controlling T cell help during positive selection"],"prefix":"10.64898","author":[{"given":"Jhon R.","family":"Enterina","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2E1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung-Yao","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2E1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liany","family":"Luna-Dulcey","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susmita","family":"Sarkar","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward N.","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeinab","family":"Jame-Chenarboo","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kennedy","family":"Chisholm","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2E1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arbab","family":"Ul Haq","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris D.","family":"St. Laurent","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2E1, 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