{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T16:08:13Z","timestamp":1778602093860,"version":"3.51.4"},"posted":{"date-parts":[[2026,5,9]]},"group-title":"Neuroscience","reference-count":45,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T00:00:00Z","timestamp":1778284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>The brain\u2019s metabolic demands are well established, but how metabolism is coordinated across anatomically distinct regions remains poorly understood. Here, using matrix-assisted laser desorption\/ionization (MALDI) imaging integrated with the Allen Brain Atlas and optimal transport-based computational analysis, we map the spatial metabolome across twelve major mouse brain divisions. We define an optimal-transport-derived inter-regional metabolite similarity metric and refer to it as metabolic coherence. This structure is largely preserved in an amyloid mouse model of Alzheimer\u2019s disease despite widespread changes in individual metabolite and lipid levels. Individual metabolites and lipids shift in a coordinated manner across regions, sustaining inter-regional relationships even as absolute levels change in patterns indicative of mitochondrial dysfunction. To test whether the coherence metric is responsive to local intervention, we targeted the left hippocampus of mice from this model via lentiviral shHIF1\u03b1 knockdown or neuronal AAV-mediated AOX expression. Both interventions were associated with metabolite normalization at the injection site. More importantly, normalization extended across distal regions sharing high metabolic similarity with the hippocampus and was accompanied by improved social memory in a single behavioral assay. Gene modulation and amyloid plaque reduction localized to the injection site.<\/jats:p>","DOI":"10.64898\/2026.05.07.723592","type":"posted-content","created":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T07:45:12Z","timestamp":1778399112000},"source":"Crossref","is-referenced-by-count":0,"title":["Metabolic Coherence of the Mouse Brain"],"prefix":"10.64898","author":[{"given":"Zizhen","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Biochemistry & Molecular Biology, College of Medicine, University of Florida, Gainesville, FL, USA"},{"name":"Center for Advanced Spatial Biomolecule Research, University of Florida, Gainesville, FL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Biochemistry & Molecular Biology, College of Medicine, University of Florida, Gainesville, FL, USA"},{"name":"Department of Biostatistics, College of Public Health and Health 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