{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:33:57Z","timestamp":1785429237270,"version":"3.56.0"},"posted":{"date-parts":[[2026,5,12]]},"group-title":"Immunology","reference-count":59,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"award":["R01 NS 103212"],"award-info":[{"award-number":["R01 NS 103212"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01 NS122174"],"award-info":[{"award-number":["R01 NS122174"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["T32 AI170478"],"award-info":[{"award-number":["T32 AI170478"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The impact of microglia antigen presentation on CNS infiltrating CD8 T cells responses during neurotropic virus infection has been difficult to define. Using Theiler\u2019s murine encephalomyelitis virus (TMEV) infection of neurons as a model system, our laboratory has previously determined that H-2D\n                  <jats:sup>b<\/jats:sup>\n                  restricted, but not H-2K\n                  <jats:sup>b<\/jats:sup>\n                  restricted CD8 T cells are required for viral clearance, demonstrating the role of discrete MHC class I alleles. To determine the extent microglia antigen presentation impacts brain-infiltrating CD8 T cells, our laboratory generated novel single MHC class I conditional knockout mice in which H-2K\n                  <jats:sup>b<\/jats:sup>\n                  or H-2D\n                  <jats:sup>b<\/jats:sup>\n                  can be specifically deactivated in TMEM119+ microglia with tamoxifen administration. During TMEV infection, conditional knockout of H-2K\n                  <jats:sup>b<\/jats:sup>\n                  in microglia reduced antigen-specific CD8 T cell proliferation in the brain. Meanwhile, mice with deletion of D\n                  <jats:sup>b<\/jats:sup>\n                  in microglia had reduced levels of perforin in antigen-specific CD8 T cells. Furthermore, microglia specific deletion of H-2D\n                  <jats:sup>b<\/jats:sup>\n                  reduced CD8 T cell numbers in the brain and preserved blood-brain barrier (BBB) integrity. Microglial D\n                  <jats:sup>b<\/jats:sup>\n                  restricted antigen presentation was also essential for the reactivation of CD8 tissue resident memory (TRM) cells and BBB integrity during memory recall responses. These findings further our understanding of how brain infiltrating antiviral CD8 T cell responses are impacted by microglia, as well as define how this cellular interaction contributes to BBB disruption during neuroinflammation. These findings also have high significance to our understanding of how microglia impact CD8 TRM cell populations that reside in the brain long after virus infection is cleared.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Graphical abstract<\/jats:title>\n                  <jats:p>\n                    <jats:fig id=\"ufig1\" position=\"float\" orientation=\"portrait\" fig-type=\"figure\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"722741v1_ufig1\" position=\"float\" orientation=\"portrait\"\/>\n                    <\/jats:fig>\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.05.08.722741","type":"posted-content","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T20:40:22Z","timestamp":1778618422000},"source":"Crossref","is-referenced-by-count":0,"title":["TMEM119+ microglia MHC class I restricted antigen presentation impacts CD8 T cell memory, effector status, and blood-brain barrier disruption during neurotropic virus infection"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1425-870X","authenticated-orcid":false,"given":"Marina","family":"Seady","sequence":"first","affiliation":[{"name":"Mayo Clinic Department of Immunology, Rochester, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A.","family":"Maynes","sequence":"additional","affiliation":[{"name":"Mayo Clinic Department of Immunology, Rochester, MN"},{"name":"Graduate School of Biomedical Sciences, Mayo Clinic, Rochester, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javonte S.","family":"Thelwell","sequence":"additional","affiliation":[{"name":"Graduate School of Biomedical Sciences, Mayo Clinic, Rochester, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Jin","sequence":"additional","affiliation":[{"name":"Mayo Clinic Department of Immunology, Rochester, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Mayo Clinic Department of Immunology, Rochester, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadley E.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Mayo Clinic Department of Immunology, Rochester, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryann K.","family":"Witter","sequence":"additional","affiliation":[{"name":"Mayo Clinic Department of Immunology, Rochester, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carley A.","family":"Owens","sequence":"additional","affiliation":[{"name":"Mayo Clinic Department of Immunology, Rochester, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma","family":"Hassani","sequence":"additional","affiliation":[{"name":"Mayo Clinic Department of Immunology, Rochester, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cody L.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Mayo Clinic Department of Immunology, Rochester, MN"},{"name":"Graduate School of Biomedical Sciences, Mayo Clinic, Rochester, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Forston","sequence":"additional","affiliation":[{"name":"Mayo Clinic Department of Immunology, Rochester, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron J.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Mayo Clinic Department of Immunology, Rochester, MN"},{"name":"Mayo Clinic Department of Neurology, Rochester, MN"},{"name":"Mayo Clinic Department of Molecular Medicine, Rochester, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026051501501327000_2026.05.08.722741v1.1","doi-asserted-by":"crossref","first-page":"e0018523","DOI":"10.1128\/mmbr.00185-23","article-title":"Unraveling the complexities of neurotropic virus infection and immune evasion","volume":"89","year":"2025","journal-title":"Microbiol Mol Biol Rev"},{"key":"2026051501501327000_2026.05.08.722741v1.2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-015-1511-3"},{"key":"2026051501501327000_2026.05.08.722741v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2023.05.009"},{"key":"2026051501501327000_2026.05.08.722741v1.4","doi-asserted-by":"crossref","unstructured":"Kimura, K. et al. 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