{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:58:52Z","timestamp":1785437932924,"version":"3.56.0"},"posted":{"date-parts":[[2026,5,13]]},"group-title":"Immunology","reference-count":66,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"NIH","award":["NIH U19AI110483"],"award-info":[{"award-number":["NIH U19AI110483"]}]},{"name":"NIH","award":["NIH P01AI12580"],"award-info":[{"award-number":["NIH P01AI12580"]}]},{"award":["GTSA"],"award-info":[{"award-number":["GTSA"]}],"id":[{"id":"https:\/\/ror.org\/02hkvsj16","id-type":"ROR","asserted-by":"publisher"}]},{"award":["Empowering Lupus Research Career Development Award"],"award-info":[{"award-number":["Empowering Lupus Research Career Development Award"]}],"id":[{"id":"https:\/\/ror.org\/02hkvsj16","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  To understand the dysregulation of autoreactive B cells in SLE, we tracked Ro60-specific cells in seropositive (SP) and seronegative (SN) patients and healthy donors (HD), using flow cytometry and monoclonal antibodies. Consistent with permissive central tolerance, Ro60\n                  <jats:sup>+<\/jats:sup>\n                  na\u00efve B cells were present in all groups with increased anergy in HD. HD and SN SLE also had greatly decreased or absent Ro60\n                  <jats:sup>+<\/jats:sup>\n                  memory and ASC, which were greatly increased in active SP SLE, thereby indicating defective distal tolerance in the latter group. Notably, Ro60 autoreactivity was strictly purged from na\u00efve-derived extra-follicular B cells in HD and SN SLE, but expanded in SP SLE, suggesting the importance of autoreactivity censoring in this pathway. SLE clustering of the distribution of Ro60\n                  <jats:sup>+<\/jats:sup>\n                  B cells identified disease heterogeneity in tolerance enforcement in SLE. Finally, we demonstrate a much higher degree of polyreactivity against other lupus antigens in SLE Ro60\n                  <jats:sup>+<\/jats:sup>\n                  na\u00efve cells, which is greatly attenuated in memory cells. Our work represents the first systematic study of antigen-specific autoreactive B cells and ASC in SLE. It enhances our understanding of human B cell tolerance and defines new approaches to measuring autoimmune activity in the course of SLE, including the assessment of immune resetting after B cell depletion therapies.\n                <\/jats:p>","DOI":"10.64898\/2026.05.08.723865","type":"posted-content","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T15:55:19Z","timestamp":1778687719000},"source":"Crossref","is-referenced-by-count":0,"title":["Dysregulation of anti-Ro60 B cell autoreactivity in systemic lupus erythematosus"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6632-6837","authenticated-orcid":false,"given":"Oindrila","family":"Rahaman","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Rheumatology, Lowance Center for Human Immunology, Emory University School of Medicine, Atlanta, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Castrillon","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Rheumatology, Lowance Center for Human Immunology, Emory University School of Medicine, Atlanta, GA"},{"name":"Department of Medicine, Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, School of Medicine, Emory University, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina","family":"Bugrovsky","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Rheumatology, Lowance Center for Human Immunology, Emory University School of Medicine, Atlanta, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raksha","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Rheumatology, Lowance Center for Human Immunology, Emory University School of Medicine, Atlanta, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Midushi","family":"Ghimire","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Rheumatology, Lowance Center for Human Immunology, Emory University School of Medicine, Atlanta, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trinh T.P","family":"Van","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Rheumatology, Lowance Center for Human Immunology, Emory University School of Medicine, Atlanta, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Rheumatology, Lowance Center for Human Immunology, Emory University School of Medicine, Atlanta, GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabeena Y","family":"Usman","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division 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