{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:41:17Z","timestamp":1785426077635,"version":"3.56.0"},"posted":{"date-parts":[[2026,5,13]]},"group-title":"Bioengineering","reference-count":35,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["U01 HL142518"],"award-info":[{"award-number":["U01 HL142518"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"award":["ANR-22-CPJ1-0090-01"],"award-info":[{"award-number":["ANR-22-CPJ1-0090-01"]}],"id":[{"id":"https:\/\/ror.org\/00rbzpz17","id-type":"ROR","asserted-by":"publisher"}]},{"award":["ANR-24-CE19-7688"],"award-info":[{"award-number":["ANR-24-CE19-7688"]}],"id":[{"id":"https:\/\/ror.org\/00rbzpz17","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001674","name":"Fondation Leducq","doi-asserted-by":"crossref","award":["22CVD03"],"award-info":[{"award-number":["22CVD03"]}],"id":[{"id":"10.13039\/501100001674","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Dissection of the thoracic aorta includes delamination of medial lamellae and permeation of blood within the media. Quantifying how biaxial loading of a vulnerable wall and fluid mechanics interact to drive dissection remains a central challenge. Here we combine controlled distension-extension testing of intact porcine descending thoracic aortas with forced intramural fluid injection to investigate how axial stretch, injection rate, and needle gauge modulate the initiation and propagation of intramural delamination. Across experiments, injection pressure\u2013volume curves exhibited nonlinear responses characterized by pressure peaks followed by stepwise pressure drops, suggesting progressive micro-delamination events within the medial lamellar networks. Increasing axial stretch significantly elevated peak injection pressure and promoted preferential axial propagation of the permeation \/ delamination front. Higher injection rates induced abrupt lamellar separation and larger dissected areas, whereas smaller needle gauges generated higher upstream pressures due to increased hydraulic resistance. Synchrotron imaging revealed the microstructural transition from intralamellar fluid permeation and wall swelling to the formation of a large fluid-filled delamination cavity. These results support a mechanistic framework in which the introduction of pressurized fluid within the aortic media behaves as a hydraulic fracture process in a layered poroelastic tissue, governed by balance across fluid pressurization, wall loading, and interlamellar strength. The findings provide quantitative insight into the biomechanical conditions that contribute to the initiation and propagation of aortic dissection.<\/jats:p>","DOI":"10.64898\/2026.05.10.721415","type":"posted-content","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T22:50:13Z","timestamp":1778712613000},"source":"Crossref","is-referenced-by-count":0,"title":["Controlled intramural fluid injection to quantify propensity to thoracic aortic dissection"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6021-8920","authenticated-orcid":false,"given":"C.","family":"Cavinato","sequence":"first","affiliation":[{"name":"LMGC, Univ. Montpellier, CNRS, Montpellier, France"},{"name":"Department of Biomedical Engineering, Yale University, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8064-8496","authenticated-orcid":false,"given":"B.","family":"Pierrat","sequence":"additional","affiliation":[{"name":"Mines Saint-Etienne, Univ Lyon, Univ Jean Monnet, INSERM, U 1059, Sainbiose, Centre CIS, 42023 Saint-Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0047-3249","authenticated-orcid":false,"given":"E.","family":"Ban","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Yale University, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Scheel","sequence":"additional","affiliation":[{"name":"Synchrotron SOLEIL, 91192 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9760-1210","authenticated-orcid":false,"given":"M.","family":"Simon","sequence":"additional","affiliation":[{"name":"Mines Saint-Etienne, Univ Lyon, Univ Jean Monnet, INSERM, U 1059, Sainbiose, Centre CIS, 42023 Saint-Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1011-2025","authenticated-orcid":false,"given":"J.D.","family":"Humphrey","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Yale University, New Haven, CT, USA"},{"name":"Vascular Biology and Therapeutics Program, Yale School of Medicine, New Haven, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026051607401458000_2026.05.10.721415v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.53"},{"key":"2026051607401458000_2026.05.10.721415v1.2","doi-asserted-by":"publisher","DOI":"10.1161\/HCI.0000000000000075"},{"key":"2026051607401458000_2026.05.10.721415v1.3","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehac757"},{"key":"2026051607401458000_2026.05.10.721415v1.4","doi-asserted-by":"publisher","DOI":"10.1109\/RBME.2019.2950140"},{"key":"2026051607401458000_2026.05.10.721415v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2019.08.017"},{"key":"2026051607401458000_2026.05.10.721415v1.6","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000087009.16755.E4"},{"key":"2026051607401458000_2026.05.10.721415v1.7","first-page":"329","article-title":"Beitr\u00e4ge zur Frage des Aneurysma dissecans","volume":"67","year":"1920","journal-title":"Beitr Pathol Anat Allg Pathol"},{"key":"2026051607401458000_2026.05.10.721415v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.xjon.2020.12.012"},{"key":"2026051607401458000_2026.05.10.721415v1.9","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000087009.16755.E4"},{"key":"2026051607401458000_2026.05.10.721415v1.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2022.10.060"},{"key":"2026051607401458000_2026.05.10.721415v1.11","doi-asserted-by":"publisher","DOI":"10.26502\/fccm.92920146"},{"issue":"5","key":"2026051607401458000_2026.05.10.721415v1.12","first-page":"569","article-title":"Tear propagation in isolated, pressurized porcine thoracic aortas","volume":"15","year":"1999","journal-title":"The Canadian Journal of Cardiology"},{"issue":"4","key":"2026051607401458000_2026.05.10.721415v1.13","first-page":"308","article-title":"Variations in strength of the porcine aorta as a function of location","volume":"17","year":"1994","journal-title":"Clinical and Investigative Medicine. 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