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Here, we performed pangenome analysis on 695 genetically representative isolates of\n                  <jats:italic>C. auris<\/jats:italic>\n                  and found that only 2.6% of gene families varied in their presence, mainly consisting of gene loss events or clade-specific genes, highlighting the clonal nature of these strains. Using microbial genome-wide association, we identified two loci associated with altered keratinocyte adherence, including genes with unknown function. To look broadly at gene function, we developed a genome-scale insertional mutagenesis approach and identified 614 high-confidence essential genes. Nearly one-third of these genes exhibited divergent essentiality compared to the model yeasts\n                  <jats:italic>Candida albicans<\/jats:italic>\n                  and\n                  <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                  . Together, this study highlights organism-specific biology and provides a comprehensive resource for identifying species-specific determinants of virulence and targets for antifungal drug development.\n                <\/jats:p>","DOI":"10.64898\/2026.05.12.724661","type":"posted-content","created":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T05:50:15Z","timestamp":1778737815000},"source":"Crossref","is-referenced-by-count":1,"title":["Pangenome and genome-scale essentiality in the emerging fungal pathogen\n                  <i>Candidozyma auris<\/i>"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9130-5269","authenticated-orcid":false,"given":"Joseph J","family":"Hale","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School; Ann Arbor MI 48019 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5967-9535","authenticated-orcid":false,"given":"Ajay J","family":"Larkin","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School; Ann Arbor MI 48019 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0501-4615","authenticated-orcid":false,"given":"Jackson R","family":"Rapala","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School; Ann Arbor MI 48019 USA"},{"name":"Gilbert S. Omenn Department of Computational Medicine and Bioinformatics, University of Michigan Medical School; Ann Arbor MI 48019 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Hurto","sequence":"additional","affiliation":[{"name":"Department of Biological Chemistry, University of Michigan Medical School; Ann Arbor MI 48019 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9605-0704","authenticated-orcid":false,"given":"Guolei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School; Ann Arbor MI 48019 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1342-8062","authenticated-orcid":false,"given":"Brynn E","family":"Elson","sequence":"additional","affiliation":[{"name":"Program in Immunology, University of Michigan Medical School; Ann Arbor MI 48019 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5821-4226","authenticated-orcid":false,"given":"Lydia","family":"Freddolino","sequence":"additional","affiliation":[{"name":"Gilbert S. Omenn Department of Computational Medicine and Bioinformatics, University of Michigan Medical School; Ann Arbor MI 48019 USA"},{"name":"Department of Biological Chemistry, University of Michigan Medical School; Ann Arbor MI 48019 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8409-278X","authenticated-orcid":false,"given":"Evan S.","family":"Snitkin","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School; Ann Arbor MI 48019 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8901-0154","authenticated-orcid":false,"given":"Teresa R","family":"O\u2019Meara","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Michigan Medical School; Ann Arbor MI 48019 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026091112453418000_2026.05.12.724661v2.1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1012011"},{"key":"2026091112453418000_2026.05.12.724661v2.2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00053-22"},{"key":"2026091112453418000_2026.05.12.724661v2.3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006290"},{"key":"2026091112453418000_2026.05.12.724661v2.4","first-page":"728","article-title":"Updated genomic epidemiologic description of Candida (Candidozyma) auris, United States","volume":"32","year":"2026","journal-title":"Emerg. 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