{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:09:22Z","timestamp":1785431362270,"version":"3.56.0"},"posted":{"date-parts":[[2026,5,15]]},"group-title":"Developmental Biology","reference-count":83,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01NS119760"],"award-info":[{"award-number":["R01NS119760"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["P30CA021765"],"award-info":[{"award-number":["P30CA021765"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/03rx10x31","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Cell fate commitment is commonly thought to entail progressive restriction of developmental potential, enforced by passive, heterochromatin-based silencing of alternative lineage programs. Here we show that maintenance of neural identity during cerebellum development instead requires active repression of a starkly divergent fate by the TEAD\u2013INSM1 transcriptional complex. Loss of TEAD1\/2 or INSM1 activates the myogenic master regulator\n                  <jats:italic>Myod1<\/jats:italic>\n                  , resulting in neural cells acquiring transcriptional, structural, and metabolic features of skeletal muscle cells. Deletion of\n                  <jats:italic>Myod1<\/jats:italic>\n                  fully suppresses neural-to-muscle conversion while partially rescuing neural developmental defects. Our results uncover a latent alternative lineage during neurodevelopment and a surprising role for sequence-specific transcription factors in enforcing lineage boundaries, including those previously thought essentially unbreachable, with implications for understanding aberrant differentiation in disease contexts and cell-type evolution.\n                <\/jats:p>","DOI":"10.64898\/2026.05.13.725001","type":"posted-content","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T08:40:21Z","timestamp":1778834421000},"source":"Crossref","is-referenced-by-count":0,"title":["Active repression of muscle fate preserves neural lineage identity during cerebellum development"],"prefix":"10.64898","author":[{"given":"Nurunnabi Mirja","family":"Shaikh","sequence":"first","affiliation":[{"name":"Department of Developmental Neurobiology, St. Jude Children\u2019s Research Hospital; Memphis, TN 38105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venkata","family":"Thulabandu","sequence":"additional","affiliation":[{"name":"Department of Developmental Neurobiology, St. Jude Children\u2019s Research Hospital; Memphis, TN 38105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Inoue","sequence":"additional","affiliation":[{"name":"Department of Developmental Neurobiology, St. Jude Children\u2019s Research Hospital; Memphis, TN 38105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Par\u00e9","sequence":"additional","affiliation":[{"name":"Department of Developmental Neurobiology, St. Jude Children\u2019s Research Hospital; Memphis, TN 38105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jackie","family":"Norrie","sequence":"additional","affiliation":[{"name":"Department of Developmental Neurobiology, St. Jude Children\u2019s Research Hospital; Memphis, TN 38105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Applied Bioinformatics, St. Jude Children\u2019s Research Hospital; Memphis, TN 38105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0099-858X","authenticated-orcid":false,"given":"Beisi","family":"Xu","sequence":"additional","affiliation":[{"name":"Center for Applied Bioinformatics, St. Jude Children\u2019s Research Hospital; Memphis, TN 38105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0651-5243","authenticated-orcid":false,"given":"Xinwei","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Developmental Neurobiology, St. Jude Children\u2019s Research Hospital; Memphis, TN 38105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026051818151374000_2026.05.13.725001v1.1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adp2959"},{"key":"2026051818151374000_2026.05.13.725001v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08533"},{"key":"2026051818151374000_2026.05.13.725001v1.3","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/bs.ctdb.2015.11.017","article-title":"Turning One Cell Type into Another","volume":"117","year":"2016","journal-title":"Current topics in developmental biology"},{"key":"2026051818151374000_2026.05.13.725001v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-021-00335-z"},{"key":"2026051818151374000_2026.05.13.725001v1.5","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-23-0225"},{"key":"2026051818151374000_2026.05.13.725001v1.6","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-022-00483-w"},{"key":"2026051818151374000_2026.05.13.725001v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2023.02.007"},{"key":"2026051818151374000_2026.05.13.725001v1.8","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau0583"},{"key":"2026051818151374000_2026.05.13.725001v1.9","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-021-00776-w"},{"key":"2026051818151374000_2026.05.13.725001v1.10","doi-asserted-by":"crossref","unstructured":"J. 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