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Here, we report ExR of pathology (ExRPath), which attains \u223c20 nm resolution and decrowding of such tissues, through iterative 20x expansion, adapted to human brain pathology specimens. We also report a single-shot 15x expansion protocol for such tissues (15ExMPath), achieved through one-shot 15x expansion. Applying ExRPath and 15ExMPath to COVID-19-decedent brain tissue reveals periodic amyloid nanoclusters that co-localize with SARS-CoV-2 in a rare minority of patient specimens, pointing to a potential neuroinflammatory phenotype associated with COVID-19, and highlighting the power of high-throughput nanoimaging, empowered by expansion microscopy, for discovering potential novel disease mechanisms.<\/jats:p>","DOI":"10.64898\/2026.05.14.725177","type":"posted-content","created":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T03:40:14Z","timestamp":1778902814000},"source":"Crossref","is-referenced-by-count":0,"title":["Expansion Revealing of Pathology Resolves Nanostructures Associated with Inflammatory Phenotypes in COVID-19 Decedent Human Brain Tissue"],"prefix":"10.64898","author":[{"given":"Alice E.","family":"Stanton","sequence":"first","affiliation":[{"name":"Koch Institute, MIT, Cambridge, MA, 02139, USA"},{"name":"Current address: Department of Neurology, Harvard Medical School, and the Center for Genomic Medicine, Massachusetts General Hospital, Boston, MA, 02114, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6116-3477","authenticated-orcid":false,"given":"Jinyoung","family":"Kang","sequence":"additional","affiliation":[{"name":"McGovern Institute for Brain Research, MIT, Cambridge, MA, 02139, USA"},{"name":"Current address: Department of Neuroscience, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, Jupiter, FL, 33458, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joel W.","family":"Blanchard","sequence":"additional","affiliation":[{"name":"Picower Institute for Learning and Memory, MIT, Cambridge, MA, 02139, USA"},{"name":"Current address: Department of Neuroscience, Black Family Stem Cell Institute, Ronald M. 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