{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:50:39Z","timestamp":1785433839571,"version":"3.56.0"},"posted":{"date-parts":[[2026,5,21]]},"group-title":"Biochemistry","reference-count":77,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T00:00:00Z","timestamp":1779321600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["R21 DC020993"],"award-info":[{"award-number":["R21 DC020993"]}],"id":[{"id":"https:\/\/ror.org\/04mhx6838","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,21]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Voice disorders affect nearly 20 million Americans and cost more than $13 billion annually. Vocal fold (VF) fibrosis, a major cause of chronic dysphonia, disrupts normal vocal fold vibration by replacing the flexible extracellular matrix with stiff fibrotic tissue. Although TGF-\u03b2 drives fibrosis, it also activates intrinsic negative feedback mechanisms, including SMAD7 induction and SMAD3 downregulation, to restrain excessive signaling. Broad inhibition of TGF-\u03b2 or canonical SMAD signaling may disrupt these protective feedback loops and impair normal tissue homeostasis. An ideal anti-fibrotic strategy should differentially target the pro-fibrotic output of TGF-\u03b2. Here, we show YAP\/TAZ inhibition selectively suppresses pro-fibrotic TGF-\u03b2 signaling in VF fibroblasts. Pharmacologic inhibition of YAP\/TAZ blocked TGF-\u03b2-induced fibroblast activation and fibrotic gene expression, while only modestly affecting canonical SMAD feedback responses. Integrated RNA-seq and ChIP-seq analyses demonstrated YAP\/TAZ primarily regulate non-canonical TGF-\u03b2 signaling and pro-fibrotic transcriptional programs. In a rat model of VF fibrosis, YAP\/TAZ inhibition reduced nuclear YAP\/TAZ localization and attenuated scar formation. Together, these findings identify YAP\/TAZ inhibition as a promising therapeutic strategy for VF fibrosis and other fibrotic diseases.<\/jats:p>","DOI":"10.64898\/2026.05.18.726086","type":"posted-content","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T10:00:16Z","timestamp":1779357616000},"source":"Crossref","is-referenced-by-count":0,"title":["YAP\/TAZ inhibition refines TGF-\u03b2 signaling to prevent laryngeal fibrosis"],"prefix":"10.64898","author":[{"given":"Ryosuke","family":"Nakamura","sequence":"first","affiliation":[{"name":"Otolaryngology-Head and Neck Surgery, NYU Grossman School of Medicine, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renjie","family":"Bing","sequence":"additional","affiliation":[{"name":"Otolaryngology-Head and Neck Surgery, NYU Grossman School of Medicine, New York, 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