{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:16:43Z","timestamp":1785417403226,"version":"3.56.0"},"posted":{"date-parts":[[2026,5,20]]},"group-title":"Gastroenterology","reference-count":40,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["K01DK127008"],"award-info":[{"award-number":["K01DK127008"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["F32DK117585"],"award-info":[{"award-number":["F32DK117585"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["P30DK097948"],"award-info":[{"award-number":["P30DK097948"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["DK055812"],"award-info":[{"award-number":["DK055812"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["DK091222"],"award-info":[{"award-number":["DK091222"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["DK097948"],"award-info":[{"award-number":["DK097948"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["P01DK091222"],"award-info":[{"award-number":["P01DK091222"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R21DK118373"],"award-info":[{"award-number":["R21DK118373"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,6,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:sec>\n                  <jats:title>Background and Aims<\/jats:title>\n                  <jats:p>Diet plays a critical role in managing Crohn\u2019s disease (CD) inflammation. We assessed whether dietary replacement of animal protein (AnimalP) by soy-pea protein (SoyP) decreases the pro-inflammatory potential of gut microbiota and intestinal inflammation in CD patients.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Design<\/jats:title>\n                  <jats:p>In an open-label, randomized controlled feeding trial at University Hospitals Cleveland Medical Center, CD participants and healthy controls were randomized (1:1) to a soy-pea or animal protein diet for 7-days. Primary outcomes were the absolute difference (\u0394d7-d0) in; Crohn\u2019s Disease Activity Index (CDAI) score and fecal myeloperoxidase (MPO). Secondary outcomes included fecal calprotectin (FC) and high-sensitivity C-reactive protein (hsCRP). Murine fecal transplantation experiments were performed to determine the inflammatory potential of diet-altered gut microbiota.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The study randomized 66 participants, of whom 60 were included in the final analysis (n=31 CD, n=29 HC). After 7 days, CD participants assigned to the SoyP diet were more likely than those assigned to the AnimalP diet to demonstrate reductions in fecal MPO (RR=2.30, 95% CI: 1.04-4.85, P=0.032) and HBI (RR=4.68, 95% CI: 1.22-17.98, P=0.009). The association between SoyP and reduced fecal MPO remained significant after Bonferroni adjustment (FWER-adjusted P=0.006). A similar directional trend was observed for CDAI (RR=1.52, 95% CI: 0.89\u20132.58, P=0.135), although this did not reach statistical significance. No participants experienced worsening of CDAI. The exploratory post hoc rank-based CDAI-MPO composite score was lower in the CD-SoyP versus CD-AnimalP group (median [IQR]: 5 [4-6] vs 8 [7-9]; P=0.012). Stratified analyses demonstrated significant reductions in fecal MPO among CD participants with lower baseline disease activity (CDAI &lt;150; P&lt;0.0001), but not among those with higher disease activity (P=0.799).<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>\n                    Short-term addition of plant-based soy-pea protein within a controlled diet was associated with reductions in objective inflammatory markers in CD, with evidence of greater effects among participants with lower baseline disease activity. ClinicalTrials.gov, Number\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"clintrialgov\" xlink:href=\"NCT04065048\">NCT04065048<\/jats:ext-link>\n                    .\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.05.20.26353678","type":"posted-content","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T18:18:08Z","timestamp":1779301088000},"source":"Crossref","is-referenced-by-count":0,"title":["A Randomized Controlled Trial Comparing Soy-Pea Protein to Animal Protein in Adults with Crohn\u2019s Disease"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8369-919X","authenticated-orcid":false,"given":"Abigail Raffner","family":"Basson","sequence":"first","affiliation":[{"name":"Department of Nutrition, Case Western Reserve University School of Medicine, Cleveland, OH, USA"},{"name":"Division of Gastroenterology & Liver Diseases, Case Western Reserve University 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