{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T18:04:04Z","timestamp":1779818644133,"version":"3.53.1"},"posted":{"date-parts":[[2026,5,22]]},"group-title":"Neuroscience","reference-count":46,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 AA015614"],"award-info":[{"award-number":["R01 AA015614"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["P50 AA022534"],"award-info":[{"award-number":["P50 AA022534"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["T32 AA014127"],"award-info":[{"award-number":["T32 AA014127"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["P20 GM109089"],"award-info":[{"award-number":["P20 GM109089"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Individuals with Fetal Alcohol Spectrum Disorders (FASDs) show reduced subicular volume, and preclinical studies compliment this by demonstrating that third-trimester-equivalent ethanol exposure induced apoptosis in corticolimbic regions, including the subiculum. The subiculum mediates hippocampal-cortical communication critical for long-term memory consolidation. Within the distal dorsal subiculum, a population of bursting neurons uniquely express VGLUT2 and they play a key role in memory processing. We hypothesized that third-trimester-equivalent ethanol exposure would reduce neuronal and VGLUT2+ cell density in the dorsal subiculum and reduce the excitability of bursting neurons, providing a mechanism for long-term memory impairments observed in FASD. To test this, postnatal day (P)7 mice received a subcutaneous injection of ethanol and long-term effects were assessed in adolescence (P35-62). Using transgenic mice with fluorescently labeled VGLUT2+ neurons, and immunohistochemistry we observed a significant reduction in neuronal density in males and an increase in VGLUT2+ cell density in females. Using whole-cell patch clamp electrophysiology, we observed a reduction in action potentials per burst in both sexes. Additionally, females showed reduced overall excitability, and a subset of neurons exhibited a shift to regular spiking. These findings suggest that development ethanol exposure disrupts subicular output by impairing burst firing, potentially weaking hippocampal-cortical communication and contributing to the cognitive deficits associated with FASD.<\/jats:p>\n                <jats:sec>\n                  <jats:title>Highlights<\/jats:title>\n                  <jats:list list-type=\"bullet\">\n                    <jats:list-item>\n                      <jats:p>Third-trimester ethanol targets VGLUT2+ neurons in the dorsal subiculum<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>Ethanol reduced neuronal density in male dorsal subiculum<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>Ethanol increases VGLUT2+ cell density in females<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>Ethanol reduces action potential per burst in both sexes<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>Females show reduced excitability and loss of bursting in some cells<\/jats:p>\n                    <\/jats:list-item>\n                  <\/jats:list>\n                <\/jats:sec>","DOI":"10.64898\/2026.05.20.726671","type":"posted-content","created":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T06:50:12Z","timestamp":1779519012000},"source":"Crossref","is-referenced-by-count":0,"title":["Third Trimester-Equivalent Alcohol Exposure Reduces Neurons in Males and Increases Vglut2\u207a Neurons with Reduced Intrinsic Bursting in Females in The Murine Dorsal Subiculum"],"prefix":"10.64898","author":[{"given":"Katalina M.","family":"Lopez","sequence":"first","affiliation":[{"name":"Department of Neurosciences, School of Medicine, University of New Mexico Health Sciences Center Albuquerque, New Mexico, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyemin","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, School of Medicine, University of New Mexico Health Sciences Center Albuquerque, New Mexico, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adeline","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, School of Medicine, University of New Mexico Health Sciences Center Albuquerque, New Mexico, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes","family":"Cazares","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, School of Medicine, University of New Mexico Health Sciences Center Albuquerque, New Mexico, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier Kelly","family":"Roman","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, School of Medicine, University of New Mexico Health Sciences Center Albuquerque, New Mexico, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenna J.","family":"Chavez","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, School of Medicine, University of New Mexico Health Sciences Center Albuquerque, New Mexico, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa Molina","family":"Garcia","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, School of Medicine, University of New Mexico Health Sciences Center Albuquerque, New Mexico, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian","family":"Jaramillo","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, School of Medicine, University of New Mexico Health Sciences Center Albuquerque, New Mexico, U.S.A"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1026-9604","authenticated-orcid":false,"given":"C. 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