{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:12:49Z","timestamp":1790874769630,"version":"4.1.0"},"posted":{"date-parts":[[2026,5,25]]},"group-title":"Developmental Biology","reference-count":33,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["AG20240665"],"award-info":[{"award-number":["AG20240665"]}],"id":[{"id":"https:\/\/ror.org\/03vpdge44","id-type":"ROR","asserted-by":"publisher"}]},{"name":"National Institute of Child Health and Human Development","award":["R37 HD014939"],"award-info":[{"award-number":["R37 HD014939"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  To facilitate the investigation of signaling by the luteinizing hormone receptor (LHR), we created a mouse line called\n                  <jats:italic>Lhr<\/jats:italic>\n                  -COIN. This line allows for the conditional replacement of the\n                  <jats:italic>Lhr<\/jats:italic>\n                  coding sequence with enhanced green fluorescent protein (eGFP), resulting in both a conditional knockout line and a reporter line. By breeding these mice with mice expressing Cre recombinase, we generated mice in which either one or both\n                  <jats:italic>Lhr<\/jats:italic>\n                  alleles were replaced with eGFP. Notably, mice in which one\n                  <jats:italic>Lhr<\/jats:italic>\n                  allele in the granulosa cells was replaced with eGFP exhibited normal LH responsiveness. This enabled live imaging of LH-induced migration of LH-receptor-expressing granulosa cells within preovulatory ovarian follicles. The\n                  <jats:italic>Lhr<\/jats:italic>\n                  -COIN mouse line holds significant potential for future research on LHR function and localization in the ovary and other tissues.\n                <\/jats:p>","DOI":"10.64898\/2026.05.21.726840","type":"posted-content","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T07:00:38Z","timestamp":1779778838000},"source":"Crossref","is-referenced-by-count":2,"title":["Conditional replacement of the mouse LH receptor with GFP, enabling imaging of cell migration during ovulation"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7409-9259","authenticated-orcid":false,"given":"Corie M.","family":"Owen","sequence":"first","affiliation":[{"name":"Department of Cell Biology, University of Connecticut Health Center, Farmington, CT 06030 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie M.","family":"Lowther","sequence":"additional","affiliation":[{"name":"Department of 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