{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T13:14:00Z","timestamp":1780319640051,"version":"3.54.1"},"posted":{"date-parts":[[2026,5,28]]},"group-title":"Cell Biology","reference-count":22,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"name":"Wilmer Eye Institute Mentored Clinician Scientist Scholar Program","award":["5K12EY015025"],"award-info":[{"award-number":["5K12EY015025"]}]},{"name":"Foundation Fighting Blindness Career Development Award"},{"name":"Retina Rising Professorship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  This protocol provides a standardized workflow for the isolation of extracellular vesicles (EVs) from mouse retinal tissue, and includes an assessment of EV size and concentration, marker expression, and EV visualization in accordance with the International Society for Extracellular Vesicles Minimal Information for Studies of Extracellular Vesicles (MISEV) guidelines. Most retinal EV studies rely on cell culture, which may not fully capture\n                  <jats:italic>in vivo<\/jats:italic>\n                  biology. Our approach more accurately reflects physiological and pathological EV states\n                  <jats:italic>in vivo<\/jats:italic>\n                  by enabling the extraction of EVs from intact retinal tissue. This method addresses a key gap in the field by providing a reproducible and rigorous protocol for studying retinal EVs in a biologically relevant context.\n                <\/jats:p>","DOI":"10.64898\/2026.05.24.724732","type":"posted-content","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T21:15:16Z","timestamp":1780002916000},"source":"Crossref","is-referenced-by-count":0,"title":["Isolation and Characterization of Extracellular Vesicles from Mouse Retina Tissue"],"prefix":"10.64898","author":[{"given":"Ishrat","family":"Ahmed","sequence":"first","affiliation":[{"name":"Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olesia","family":"Gololobova","sequence":"additional","affiliation":[{"name":"Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Amanullah","sequence":"additional","affiliation":[{"name":"Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zach","family":"Troyer","sequence":"additional","affiliation":[{"name":"Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua H.","family":"Yu","sequence":"additional","affiliation":[{"name":"Georgetown University School of Medicine, Washington, D.C"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James T.","family":"Handa","sequence":"additional","affiliation":[{"name":"Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Qian","sequence":"additional","affiliation":[{"name":"Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seth","family":"Blackshaw","sequence":"additional","affiliation":[{"name":"Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, MD, USA"},{"name":"Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth","family":"Witwer","sequence":"additional","affiliation":[{"name":"Department of Molecular and Comparative Pathobiology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA"},{"name":"Department of Neurology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026060105500461000_2026.05.24.724732v1.1","first-page":"1042469","article-title":"Extracellular vesicles in the retina - putative roles in physiology and disease","volume":"15","year":"2022","journal-title":"Front Mol 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