{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T02:08:37Z","timestamp":1780106917879,"version":"3.54.0"},"posted":{"date-parts":[[2026,5,27]]},"group-title":"Cell Biology","reference-count":71,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01GM125839"],"award-info":[{"award-number":["R01GM125839"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Academy of Sciences","award":["2042280"],"award-info":[{"award-number":["2042280"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Cell adhesion molecules of the immunoglobulin superfamily (IgCAMs) coordinate adhesive interactions with intracellular organization during tissue morphogenesis. In the\n                  <jats:italic>Drosophila<\/jats:italic>\n                  follicular epithelium, epithelial maintenance depends on reintegration, a process in which mitotically displaced cells reincorporate into the epithelial monolayer. Previous work identified the IgCAMs Fasciclin 2 (Fas2) and Neuroglian (Nrg) as parallel, partially redundant regulators of reintegration, but the intracellular mechanisms linking adhesion to reintegration remained unclear. Here, we show that Fas2 supports reintegration through two mechanistically distinct modes: a transmembrane mode and a GPI-linked mode. Although both contribute to reintegration, the transmembrane mechanism is more effective and depends on stabilization of a cortical Fas2 pool through intracellular coupling. Using yeast two-hybrid screening, genetics, and fluorescence recovery after photobleaching (FRAP), we identify the scaffold protein Discs large (Dlg1) as a functional intracellular partner of transmembrane Fas2. Partial disruption of Dlg1 preferentially sensitizes epithelia in which the parallel Nrg-dependent reintegration mechanism is compromised, consistent with Dlg1 functioning primarily within the Fas2-dependent reintegration arm. While Dlg1 is not required for Fas2 membrane localization, Dlg1 disruption increases the mobile fraction of transmembrane Fas2, indicating that Dlg1 promotes retention of a stabilized cortical Fas2 pool. Together, these findings support a model in which epithelial reintegration depends on coordinated adhesion\u2013scaffold coupling and reveal mechanistic parallels between epithelial reintegration and IgCAM-dependent processes in the developing nervous system.\n                <\/jats:p>","DOI":"10.64898\/2026.05.24.727532","type":"posted-content","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T05:45:17Z","timestamp":1779947117000},"source":"Crossref","is-referenced-by-count":0,"title":["Fasciclin 2 Cooperates with Discs Large to Maintain Epithelial Architecture"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0801-9577","authenticated-orcid":false,"given":"Tara M.","family":"Finegan","sequence":"first","affiliation":[{"name":"Division of Biological Sciences, University of Missouri, Columbia, MO, 65201, USA"},{"name":"Advanced Light Microscopy Core, University of Missouri, 120 Bond Life Sciences Center, 1201 Rollins St, Columbia, MO 65211, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3003-0984","authenticated-orcid":false,"given":"Michael W.","family":"Linhoff","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Missouri, Columbia, MO, 65201, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Rice","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Missouri, Columbia, MO, 65201, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9066-1555","authenticated-orcid":false,"given":"Sahel","family":"Ghasemzadeh","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Missouri, Columbia, MO, 65201, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8046-6299","authenticated-orcid":false,"given":"Zach","family":"Wright","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Missouri, Columbia, MO, 65201, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3583-7182","authenticated-orcid":false,"given":"Kathryn E.","family":"Neville","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rochester, Rochester NY, 14627, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tyler J.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rochester, Rochester NY, 14627, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4300-4338","authenticated-orcid":false,"given":"Evan W.","family":"Ost","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Missouri, Columbia, MO, 65201, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Lowe","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rochester, Rochester NY, 14627, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Dunivan","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Missouri, Columbia, MO, 65201, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6549-3052","authenticated-orcid":false,"given":"Oscar","family":"Andrade Mendoza","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Missouri, Columbia, MO, 65201, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7551-2460","authenticated-orcid":false,"given":"Christian M.","family":"Cammarota","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Rochester, Rochester NY, 14627, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1689-3715","authenticated-orcid":false,"given":"Dan T.","family":"Bergstralh","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, University of Missouri, Columbia, MO, 65201, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"issue":"17","key":"2026052918201820000_2026.05.24.727532v1.1","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1016\/j.cub.2013.07.017","article-title":"Discs Large Links Spindle Orientation to Apical-Basal Polarity in Drosophila Epithelia","volume":"23","year":"2013","journal-title":"Current Biology : CB"},{"key":"2026052918201820000_2026.05.24.727532v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3248"},{"key":"2026052918201820000_2026.05.24.727532v1.3","doi-asserted-by":"crossref","unstructured":"Bilder, D. , M. 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