{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T23:15:45Z","timestamp":1787786145014,"version":"build-2784847793"},"posted":{"date-parts":[[2026,5,26]]},"group-title":"Neuroscience","reference-count":61,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T00:00:00Z","timestamp":1779753600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"United States National Institutes of Health","award":["R01-NS121542"],"award-info":[{"award-number":["R01-NS121542"]}]},{"name":"United States National Institutes of Health","award":["R21-NS108508"],"award-info":[{"award-number":["R21-NS108508"]}]},{"name":"United States National Institutes of Health","award":["R21-NS120315"],"award-info":[{"award-number":["R21-NS120315"]}]},{"name":"United States National Institutes of Health","award":["R21-NS097913"],"award-info":[{"award-number":["R21-NS097913"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,5,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Loss-of-function variants of\n                  <jats:italic>MECP2<\/jats:italic>\n                  cause Rett syndrome; however, their impact on cerebellar computations for learning remains poorly understood. Here, we show that\n                  <jats:italic>Mecp2<\/jats:italic>\n                  deletion specifically in Purkinje cells does not broadly disrupt cerebellar-dependent behaviors but selectively compromises those that require precise timing, coordination, and associative updating in the cerebellar cortex.\n                  <jats:italic>Mecp2<\/jats:italic>\n                  loss altered the emergence of learning-related Purkinje cell activity\n                  <jats:italic>in vivo<\/jats:italic>\n                  and disrupted their intrinsic and synaptic properties that support adaptive cerebellar output. These findings identify MeCP2 as a critical player in Purkinje cell function to regulate cerebellar learning signals and suggest that Rett syndrome-related motor dysfunction reflects impaired adaptive computation rather than a generalized loss of motor capacity.\n                <\/jats:p>","DOI":"10.64898\/2026.05.25.727684","type":"posted-content","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T08:45:18Z","timestamp":1779785118000},"source":"Crossref","is-referenced-by-count":1,"title":["MeCP2 is Necessary in Cerebellar Purkinje Cells for Precise Network Dynamics During Associative Motor Learning"],"prefix":"10.64898","author":[{"given":"Jenny","family":"Shen","sequence":"first","affiliation":[{"name":"Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Lopes","family":"Gon\u00e7alez","sequence":"additional","affiliation":[{"name":"Graduate Program in Behavioral Neuroscience, University of Alabama at Birmingham, Birmingham, AL 35294, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikayla","family":"Jackson","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Likhitha","family":"Polepalli","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kruthika","family":"Dheeravath","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulla","family":"Hamki","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariam","family":"Hamki","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Hicks","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6085-5527","authenticated-orcid":false,"given":"Lucas","family":"Pozzo-Miller","sequence":"additional","affiliation":[{"name":"Department of Pediatrics & Human Development, College of Human Medicine, Michigan State University, Grand Rapids, MI 49503, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA"},{"name":"Department of Pediatrics & Human Development, College of Human Medicine, Michigan State University, Grand Rapids, MI 49503, 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