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This developmental process involves an intricate gene expression regulatory network that drives gametocyte commitment, development, and the production of male and female gametes. Here, we characterized the role of the CCCH-type zinc-finger protein\n                  <jats:italic>Plasmodium falciparum<\/jats:italic>\n                  Male Development 3 (\n                  <jats:italic>Pf<\/jats:italic>\n                  MD3) in the formation of gametes. Genetic disruption of\n                  <jats:italic>Pf<\/jats:italic>\n                  MD3 revealed a male fertility defect that decreased infectivity to mosquitoes. Molecular characterization of\n                  <jats:italic>Pf<\/jats:italic>\n                  MD3 revealed its binding to mRNA transcripts associated with male gamete development through a specific RNA sequence. Gametocyte proteins encoded by these\n                  <jats:italic>Pf<\/jats:italic>\n                  MD3-bound transcripts decreased significantly in\n                  <jats:italic>Pf<\/jats:italic>\n                  MD3 knockout parasites, suggesting that\n                  <jats:italic>Pf<\/jats:italic>\n                  MD3 impacts translation. In addition to RNA-binding,\n                  <jats:italic>Pf<\/jats:italic>\n                  MD3 also interacts with proteins involved in mRNA processing and nuclear export that influence translational regulation. This study establishes\n                  <jats:italic>Pf<\/jats:italic>\n                  MD3 as an RNA-binding protein that impacts lineage-specific translation and male gamete fertility.\n                <\/jats:p>","DOI":"10.64898\/2026.05.27.726058","type":"posted-content","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T07:25:14Z","timestamp":1780039514000},"source":"Crossref","is-referenced-by-count":0,"title":["The CCCH-type zinc-finger\n                  <i>Pf<\/i>\n                  MD3 promotes translation for malaria parasite transmission"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1710-0441","authenticated-orcid":false,"given":"Ri\u00ebtte","family":"van Biljon","sequence":"first","affiliation":[{"name":"Division of Bacterial, Parasitic, and Allergenic Products, Office of Vaccines Research and Review, Center for Biologics Evaluations and Research, Food and Drug Administration, Silver Spring, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suyash","family":"Bhatnagar","sequence":"additional","affiliation":[{"name":"Division of Bacterial, Parasitic, and Allergenic Products, Office of Vaccines Research and Review, Center for Biologics Evaluations and Research, Food and Drug Administration, Silver Spring, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajat","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry & Molecular Biology and the Huck Center for Malaria Research, Pennsylvania State University, University Park, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7173-4325","authenticated-orcid":false,"given":"Megan","family":"Gliozzi","sequence":"additional","affiliation":[{"name":"Division of Bacterial, Parasitic, and Allergenic Products, Office of Vaccines Research and Review, Center for Biologics Evaluations and Research, Food and Drug Administration, Silver Spring, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoyue","family":"Xu","sequence":"additional","affiliation":[{"name":"Johns Hopkins Malaria Research Institute and Department of Molecular Microbiology & Immunology, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhai","family":"Tripathi","sequence":"additional","affiliation":[{"name":"Johns Hopkins Malaria Research Institute and Department of Molecular Microbiology & Immunology, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnab","family":"Ashrafi","sequence":"additional","affiliation":[{"name":"Johns Hopkins Malaria Research Institute and Department of Molecular Microbiology & Immunology, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristian","family":"Swearingen","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology, Seattle, WA, US"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Photini","family":"Sinnis","sequence":"additional","affiliation":[{"name":"Johns Hopkins Malaria Research Institute and Department of Molecular Microbiology & Immunology, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6173-5882","authenticated-orcid":false,"given":"Manuel","family":"Llin\u00e1s","sequence":"additional","affiliation":[{"name":"Department of Biochemistry & Molecular Biology and the Huck Center for Malaria Research, Pennsylvania State University, University Park, PA, USA"},{"name":"Department of Chemistry, Pennsylvania State University, University Park, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0264-4689","authenticated-orcid":false,"given":"Heather J.","family":"Painter","sequence":"additional","affiliation":[{"name":"Division of Bacterial, Parasitic, and Allergenic Products, Office of Vaccines Research and Review, Center for Biologics Evaluations and Research, Food and Drug Administration, Silver Spring, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026053018150794000_2026.05.27.726058v1.1","unstructured":"WHO. 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