{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:02:10Z","timestamp":1790686930012,"version":"4.1.0"},"posted":{"date-parts":[[2026,6,1]]},"group-title":"Neuroscience","reference-count":35,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["AG089761 (GAC)"],"award-info":[{"award-number":["AG089761 (GAC)"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["ES034194 (JDB)"],"award-info":[{"award-number":["ES034194 (JDB)"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["ES033333 (JDB)"],"award-info":[{"award-number":["ES033333 (JDB)"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["AG081264 (AFL)"],"award-info":[{"award-number":["AG081264 (AFL)"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Texas Alzheimer's Research and Care Consortium"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>The brain\u2019s extracellular matrix (ECM) is highly specialized and unique. Unlike the collagen-rich matrices found in other tissues, it consists of complex proteoglycans and glycosaminoglycans that play critical roles in neuroplasticity including the integration and storage of new memories. Cystatin-related epididymal spermatogenic (CRES) is a member of a reproductive subgroup of family 2 cystatins and part of a specialized functional amyloid-containing ECM with host defense functions in the epididymal lumen. CRES and the endogenous epididymal amyloid matrix exhibit high plasticity, forming distinct amyloid structures, with antimicrobial functions tailored to specific bacterial strains. Recent studies show that CRES\/CRES amyloid is also present in the mammalian brain ECM and suggest its inherent shape-shifting properties might contribute to neuroplasticity. In this study, we utilized a CRES knockout (KO) mouse model to determine if the loss of CRES altered the structure of the hippocampal and cortical ECM and impacted brain function. We report that CRES KO male and female mice exhibited sex-specific structural changes in both the loose\/interstitial and perineuronal net (PNN) ECM. Additionally, young CRES KO males\u2014but not females\u2014displayed deficits in learning, memory and executive functioning when compared to WT controls. In contrast, older CRES KO males did not exhibit behavioral deficits, a finding that correlated with the observation of a similar PNN ECM structure when compared to WT mice. Our data suggest that CRES functions as a regulatory or structural component of the brain ECM, contributing to its sex-specific structural organization and plasticity, affecting sex-specific behaviors of learning and memory.<\/jats:p>\n                <jats:sec>\n                  <jats:title>Significance statement<\/jats:title>\n                  <jats:p>Structural changes in the brain ECM drive neuroplasticity that is essential for normal function; however, these same alterations can contribute to neurodegeneration and neurological disorders. Brain ECM structure differs between males and females and promotes sex-specific behaviors; however, the full extent of sex-specific distinctions in ECM structure\/function relationships has yet to be elucidated. Using a CRES KO mouse model, we show that the functional amyloid CRES plays a regulatory\/structural role in the brain ECM. The loss of CRES resulted in sex differences in loose\/interstitial and PNN ECM organization and a male but not female learning and memory deficit. Our studies suggest that CRES\/CRES amyloid contributes to ECM neuroplasticity and remodeling processes that drive sex-specific behaviors and possibly sexually dimorphic disease.<\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.05.27.728219","type":"posted-content","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T19:15:14Z","timestamp":1780341314000},"source":"Crossref","is-referenced-by-count":0,"title":["Altered Brain Extracellular Matrix Structure and Sex-Specific Learning\/Memory Dysfunction in Mice Lacking the Functional Amyloid Cystatin-Related Epididymal Spermatogenic (CRES)"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8694-3083","authenticated-orcid":false,"given":"Alejandra","family":"Gomez","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6379-4838","authenticated-orcid":false,"given":"Aric F.","family":"Logsdon","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Neuroscience, Texas Tech University Health Sciences Center, Lubbock, TX 79430"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3767-2922","authenticated-orcid":false,"given":"Jeremy D.","family":"Bailoo","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2903-1690","authenticated-orcid":false,"given":"Gail A.","family":"Cornwall","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX 79430"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026092905501120000_2026.05.27.728219v2.1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwz060"},{"key":"2026092905501120000_2026.05.27.728219v2.2","doi-asserted-by":"crossref","first-page":"1492327","DOI":"10.3389\/fnbeh.2024.1492327","article-title":"A bespoke water T-maze apparatus and protocol: An optimized, reliable, and repeatable method for screening learning, memory, and executive functioning in laboratory mice","volume":"18","year":"2024","journal-title":"Frontiers in Behavioral Neuroscience"},{"key":"2026092905501120000_2026.05.27.728219v2.3","doi-asserted-by":"crossref","unstructured":"Batzdorf, C. 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