{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T20:00:35Z","timestamp":1780516835558,"version":"3.54.1"},"posted":{"date-parts":[[2026,6,1]]},"group-title":"Pathology","reference-count":56,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,6,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>The choroid plexus epithelial cells (CPECs) at the blood-cerebrospinal fluid (CSF) interface possess an exceptionally high mitochondrial content to support CNS homeostasis. Oncocytic CPECs (O-CPECs), characterized by enlarged and granular eosinophilic cytoplasm composed of excessive abnormal mitochondria, likely contribute to an energetic failure of this energy-demanding tissue. The relationship between O-CPECs and other CPEC pathologies in humans, such as Biondi body (BB) amyloid inclusions, remains poorly defined. In the present study, using H&amp;E-stained sections from 68 postmortem cases, we classified O-CPECs by quantitative size criteria and cytological features, and found an increase in the prevalence of O-CPECs with age after adjusting for sex and tissue source. After excluding two influential control cases, there was evidence for a further increase associated with Alzheimer\u2019s disease. Using antibodies to ATP synthase beta chain to classify O-CPECs, and thioflavin-S to identify BBs, we revealed an increased prevalence of BBs in O-CPECs compared to neighboring non-oncocytic cells. Small multiple BB inclusions were responsible for the increase in O-CPECs, while the prevalence of larger inclusions was decreased in O-CPECs. Together, our data support a clear age-associated oncocytic transformation of CPECs and implicate mitochondrial dysfunction-amyloid interactions.<\/jats:p>","DOI":"10.64898\/2026.05.27.728253","type":"posted-content","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T19:10:15Z","timestamp":1780341015000},"source":"Crossref","is-referenced-by-count":0,"title":["Age-associated oncocytic transformation correlates with an increased prevalence of small multiple Biondi body inclusions in human choroid plexus epithelial cells"],"prefix":"10.64898","author":[{"given":"Caren","family":"Yassa","sequence":"first","affiliation":[{"name":"Department of Pathology & Laboratory Medicine, University of California Irvine, Irvine, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erfan","family":"Zolfaghari","sequence":"additional","affiliation":[{"name":"Department of Pathology & Laboratory Medicine, University of California Irvine, Irvine, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Neel","sequence":"additional","affiliation":[{"name":"Department of Pathology & Laboratory Medicine, University of California Irvine, Irvine, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riley","family":"Scanlon","sequence":"additional","affiliation":[{"name":"Department of Pathology & Laboratory Medicine, University of California Irvine, Irvine, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett A.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Pathology & Laboratory Medicine, University of California Irvine, Irvine, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin S.","family":"Monuki","sequence":"additional","affiliation":[{"name":"Department of Pathology & Laboratory Medicine, University of California Irvine, Irvine, CA, USA"},{"name":"Department of Developmental & Cell Biology, University of California Irvine, Irvine, CA, USA"},{"name":"Sue and Bill Gross Stem Cell Research Center, University of California Irvine, Irvine, CA, USA"},{"name":"Alzheimer\u2019s Disease Research Center, University of California Irvine, Irvine, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026060312100695000_2026.05.27.728253v1.1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1971.00770060078009"},{"key":"2026060312100695000_2026.05.27.728253v1.2","first-page":"161","article-title":"Ein neuer histologischer befund am epithel des plexus chorioideus","volume":"144","year":"1993","journal-title":"Z Ges Neurol Psychiatry"},{"key":"2026060312100695000_2026.05.27.728253v1.3","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-012-1001-9"},{"key":"2026060312100695000_2026.05.27.728253v1.4","doi-asserted-by":"publisher","DOI":"10.2307\/1268249"},{"key":"2026060312100695000_2026.05.27.728253v1.5","first-page":"399","volume":"116","year":"1997","journal-title":"Mitochondrial content of choroid plexus epithelium: Exp Brain Res"},{"key":"2026060312100695000_2026.05.27.728253v1.6","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1093\/jnen\/59.7.621","article-title":"Neuropathological and histochemical changes in a multiple mitochondrial DNA deletion disorder","volume":"59","year":"2000","journal-title":"J Neuropathol Exp Neurol"},{"key":"2026060312100695000_2026.05.27.728253v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-4580(00)00234-7"},{"key":"2026060312100695000_2026.05.27.728253v1.8","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.57.2.260"},{"key":"2026060312100695000_2026.05.27.728253v1.9","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1046\/j.1365-2990.2001.00315.x","article-title":"Accelerated ageing changes in the choroid plexus of a case with multiple mitochondrial DNA deletions","volume":"27","year":"2001","journal-title":"Neuropathology Appl Neurobio"},{"key":"2026060312100695000_2026.05.27.728253v1.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2025.102713"},{"key":"2026060312100695000_2026.05.27.728253v1.11","first-page":"124","article-title":"Intracellular neurofibrillary tangle-like aggregations. 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