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Although immortalized cell lines remain widely used, they fail to capture the cellular complexity of the lung, while animal models\u2014though informative\u2014are often costly, low-throughput, and limited in their ability to model human disease. Here, we describe a rapid and scalable precision-cut lung slice (PCLS) platform that overcomes many of these limitations. Our workflow generates infection-ready lung tissue slices within 24 hours and maintains tissue viability over extended culture periods. Using influenza A virus as a model pathogen, we demonstrate that PCLS support robust viral replication, recapitulate characteristic infection-associated pathology, and elicit localized immune cell responses within the respiratory epithelium. Together, these features establish PCLS as a versatile and physiologically relevant platform for studying the biology of influenza virus and other respiratory pathogens.<\/jats:p>","DOI":"10.64898\/2026.05.28.728430","type":"posted-content","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T08:50:16Z","timestamp":1780044616000},"source":"Crossref","is-referenced-by-count":0,"title":["A precision-cut lung slice platform for evaluating respiratory virus replication dynamics"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4279-0625","authenticated-orcid":false,"given":"Jovanna A.","family":"Fusco","sequence":"first","affiliation":[{"name":"Department of Veterinary Biosciences, The Ohio State University, Columbus, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6585-1444","authenticated-orcid":false,"given":"Min","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Veterinary 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