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Here, combining evolutionary analysis of more than 500 sequences with cryo-electron microscopy and biophysical assays, we determine the atomic structure of chorion amyloid filaments and uncover the principles governing their assembly. Contrary to previous structural predictions, chorion filaments adopt a \u03b2-serpentine fold stabilized by a short hexapeptide motif that forms homotypic steric-zipper interfaces, self-assembles autonomously, and seeds full-length filament growth. Amyloid formation proceeds through secondary nucleation, while thermodynamic and structural analyses support a hierarchical assembly mechanism in which motif-driven interactions nucleate filament formation prior to consolidation of the mature yet lalbile protofilament core. These findings establish the molecular basis of insect egg-coat assembly and demonstrate that mechanisms commonly associated with pathological aggregation can also operate within a biologically regulated functional amyloid framework.<\/jats:p>","DOI":"10.64898\/2026.05.29.727653","type":"posted-content","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T23:30:14Z","timestamp":1780443014000},"source":"Crossref","is-referenced-by-count":2,"title":["A functional amyloid scaffold shapes insect egg coats"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0960-6274","authenticated-orcid":false,"given":"Katerina","family":"Konstantoulea","sequence":"first","affiliation":[{"name":"Center for Alzheimer\u2019s and Neurodegenerative Diseases, University of Texas Southwestern Medical Center, Dallas, TX, USA"},{"name":"Peter O\u2019Donnell Jr. Brain Institute, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA"},{"name":"Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7428-6812","authenticated-orcid":false,"given":"Peter","family":"Kunach","sequence":"additional","affiliation":[{"name":"Center for Alzheimer\u2019s and Neurodegenerative Diseases, University of Texas Southwestern Medical Center, Dallas, TX, USA"},{"name":"Peter O\u2019Donnell Jr. Brain Institute, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1595-6665","authenticated-orcid":false,"given":"Harichandra","family":"Tagad","sequence":"additional","affiliation":[{"name":"Center for Alzheimer\u2019s and Neurodegenerative Diseases, University of Texas Southwestern Medical Center, Dallas, TX, USA"},{"name":"Peter O\u2019Donnell Jr. Brain Institute, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA"},{"name":"Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8085-7770","authenticated-orcid":false,"given":"Marc I.","family":"Diamond","sequence":"additional","affiliation":[{"name":"Center for Alzheimer\u2019s and Neurodegenerative Diseases, University of Texas Southwestern Medical Center, Dallas, TX, USA"},{"name":"Peter O\u2019Donnell Jr. Brain Institute, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4030-1022","authenticated-orcid":false,"given":"Nikolaos N.","family":"Louros","sequence":"additional","affiliation":[{"name":"Center for Alzheimer\u2019s and Neurodegenerative Diseases, University of Texas Southwestern Medical Center, Dallas, TX, USA"},{"name":"Peter O\u2019Donnell Jr. Brain Institute, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA"},{"name":"Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026060500501071000_2026.05.29.727653v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03680"},{"key":"2026060500501071000_2026.05.29.727653v1.2","doi-asserted-by":"crossref","unstructured":"Otzen, D. & Riek, R. 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