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We recently identified in\n                  <jats:italic>Aspergillus nidulans<\/jats:italic>\n                  a vezatin homolog VezA that enhances dynactin assembly, but VezA does not co-localize with dynactin, suggesting a transient interaction. It was found that overexpression of p50 separates the shoulder from the mini-filament and causes late-onset neurodegeneration in mice, but its cellular effects in the context of VezA need to be studied. Here we found astonishingly that p50 overexpression in\n                  <jats:italic>A. nidulans<\/jats:italic>\n                  causes Arp11, p50 (but not p150), and VezA to form co-localized clusters whose presence depends on Arp1. Formation of the VezA cluster also depends on Arp11, and VezA significantly enhances the intensity of Arp11-GFP clusters but not that of p50-GFP clusters. Moreover, an evolutionarily conserved region of p50 (aa17-25) forming a beta-sheet structure with Arp1 as revealed in previous cryo-EM studies is critical for the formation of Arp11, p50 and VezA clusters. These results suggest that excess p50 induces cluster formation driven by p50-Arp1 interactions, and that this assembly intermediate without the full shoulder traps VezA, which is involved in the Arp11-Arp1-p50 interactions.\n                <\/jats:p>","DOI":"10.64898\/2026.05.29.728734","type":"posted-content","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T21:00:19Z","timestamp":1780434019000},"source":"Crossref","is-referenced-by-count":0,"title":["Excess p50 induces Arp1-dependent dynactin clusters containing the assembly factor VezA"],"prefix":"10.64898","author":[{"given":"Jun","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences- F. Edward H\u00e9bert School of Medicine, Bethesda, Maryland 20814, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongde","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences- F. Edward H\u00e9bert School of Medicine, Bethesda, Maryland 20814, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, The Uniformed Services University of the Health Sciences- F. Edward H\u00e9bert School of Medicine, Bethesda, Maryland 20814, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026060411151492000_2026.05.29.728734v1.1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00848.x"},{"key":"2026060411151492000_2026.05.29.728734v1.2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.107.079103"},{"key":"2026060411151492000_2026.05.29.728734v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.110598"},{"key":"2026060411151492000_2026.05.29.728734v1.4","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.2.469"},{"key":"2026060411151492000_2026.05.29.728734v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/S41586-022-05186-Y"},{"key":"2026060411151492000_2026.05.29.728734v1.6","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E14-03-0842"},{"key":"2026060411151492000_2026.05.29.728734v1.7","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2996"},{"key":"2026060411151492000_2026.05.29.728734v1.8","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-120420-104341"},{"key":"2026060411151492000_2026.05.29.728734v1.9","doi-asserted-by":"crossref","unstructured":"d\u2019Amico, E.A ., M. 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