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Here, using\n                  <jats:italic>O<\/jats:italic>\n                  -methyl-L-tyrosine (OMY) as a model compound, we establish non-canonical amino acids (ncAAs) as a tool for plant-based control of associated microbial activity. We use genetic code expansion to engineer OMY-dependent control of protein synthesis in the soil bacterium\n                  <jats:italic>Bacillus subtilis.<\/jats:italic>\n                  Then, we engineer agronomically diverse plants, including Arabidopsis, tomato and poplar, to biosynthesize OMY. We show that plant-derived OMY can stimulate gene expression in both model and wild soil bacteria while also demonstrating that inducible and tissue-specific expression of a single biosynthetic enzyme by the plant enables on-demand control over microbial activity. This work establishes ncAAs as a tool for programming plant-microbe partnerships.\n                <\/jats:p>","DOI":"10.64898\/2026.05.29.728790","type":"posted-content","created":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T04:55:20Z","timestamp":1780203320000},"source":"Crossref","is-referenced-by-count":2,"title":["Genetic code expansion enables plant-directed control of bacterial activity"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4102-292X","authenticated-orcid":false,"given":"Vivian","family":"Zhong","sequence":"first","affiliation":[{"name":"Department of Bioengineering, Stanford University, Stanford, CA 94305, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5822-0645","authenticated-orcid":false,"given":"Michaela A.","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, University of Delaware, Newark 19716, DE, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2937-7210","authenticated-orcid":false,"given":"Avaniek","family":"Cabales","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, University of Delaware, Newark 19716, DE, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9152-5077","authenticated-orcid":false,"given":"Alice","family":"Gevorgyan","sequence":"additional","affiliation":[{"name":"Department of Biology, Stanford University, Stanford, CA 94305, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6221-6443","authenticated-orcid":false,"given":"Ren\u00e9","family":"Inckemann","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Stanford University, Stanford, CA 94305, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2955-0308","authenticated-orcid":false,"given":"Anna A.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Stanford University, Stanford, CA 94305, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0993-3950","authenticated-orcid":false,"given":"Sumudu S.","family":"Karunadasa","sequence":"additional","affiliation":[{"name":"Department of Plant Biology and Carnegie Mass Spectrometry Facility, Carnegie Institution for Science, Stanford, CA 94305, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1461-5746","authenticated-orcid":false,"given":"Amanda M.","family":"Forti","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, University of Delaware, Newark 19716, DE, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6741-9506","authenticated-orcid":false,"given":"Shou-Ling","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Plant Biology and Carnegie Mass Spectrometry Facility, Carnegie Institution for Science, Stanford, CA 94305, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6869-9530","authenticated-orcid":false,"given":"Aditya M.","family":"Kunjapur","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, University of Delaware, Newark 19716, DE, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7808-4281","authenticated-orcid":false,"given":"Jennifer A. 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