{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T12:14:05Z","timestamp":1790165645107,"version":"4.1.0"},"posted":{"date-parts":[[2026,6,3]]},"group-title":"Synthetic Biology","reference-count":82,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"award":["2037822"],"award-info":[{"award-number":["2037822"]}],"id":[{"id":"https:\/\/ror.org\/011kf5r70","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/05bcgfn72","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/054d9v685","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,6,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Encapsulins are prokaryotic self-assembling protein nanocages with promise as nanovaccine scaffolds. Their utility as modular platforms require tolerance to surface engineering, high-yield soluble production, formulation stability, and controlled antigen (co-)display. Herein, a previously uncharacterized encapsulin from Alkaliphilus metalliredigens is engineered into a SpyCatcher-decorated nanoscaffold (Am-S) that enables controlled surface display of SpyTagged antigens. Cryo-EM confirms that the native encapsulin forms a\n                  <jats:italic>T<\/jats:italic>\n                  = 1 icosahedral nanocage, and that C-terminal SpyCatcher fusion yields Am-S without compromising nanocage assembly, symmetry, or structural integrity. Notably, Am-S exhibits high-yield soluble production in\n                  <jats:italic>Escherichia coli<\/jats:italic>\n                  , remains monodisperse after freeze\u2013thaw and extended storage, and supports efficient SpyTagged peptide conjugation for single- and multi-antigen display. As a proof-of-concept, Am-S is functionalized with Alzheimer\u2019s disease-associated amyloid-\u03b2 and\/or hyperphosphorylated tau epitopes to generate single-target nanocages displaying either antigen and dual-target mosaic nanocages co-displaying both. In mice, Am-S antigen display enhances antigen-specific IgG responses relative to free antigens and induces predominantly IgG1-biased humoral immunity. Mosaic nanocages elicit antibodies against both targets, with immune sera selectively recognizing amyloid-\u03b2- and phosphorylated tau-associated pathology in ex vivo brain sections from Alzheimer\u2019s disease mouse models. These findings position Am-S as a manufacturable scaffold for developing multi-targeting nanovaccines against complex diseases.\n                <\/jats:p>","DOI":"10.64898\/2026.06.01.729406","type":"posted-content","created":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T04:45:44Z","timestamp":1780548344000},"source":"Crossref","is-referenced-by-count":1,"title":["Engineering a Novel Bacterial Encapsulin for Programmable Surface Functionalization: From Single-Target to Mosaic Nanovaccines"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1442-1696","authenticated-orcid":false,"given":"India","family":"Boyton","sequence":"first","affiliation":[{"name":"Australian Institute for Microbiology and Infection, University of Technology Sydney, Gadigal Country, Sydney, NSW 2007, Australia"},{"name":"School of Life Sciences, University of Technology Sydney, Gadigal Country, Sydney, NSW 2007, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4533-6627","authenticated-orcid":false,"given":"Claire","family":"Rennie","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Technology Sydney, Gadigal Country, Sydney, NSW 2007, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9862-3016","authenticated-orcid":false,"given":"Julia","family":"van der Hoven","sequence":"additional","affiliation":[{"name":"Dementia Research Centre, Macquarie University, NSW 2109, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel Del","family":"Valle","sequence":"additional","affiliation":[{"name":"Viral Vector Manufacturing Facility, Kids Research, The Children\u2019s Hospital at Westmead, Level 4\/178 Hawkesbury Rd, Westmead, NSW 2145, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis","family":"Diaz","sequence":"additional","affiliation":[{"name":"All G Co Holdings Pty Limited, NSW 2017, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8994-5235","authenticated-orcid":false,"given":"Juanfang","family":"Ruan","sequence":"additional","affiliation":[{"name":"Electron Microscope Unit, Mark Wainwright Analytical Centre, University of New South Wales Sydney, NSW 2052, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0151-6020","authenticated-orcid":false,"given":"Daniel","family":"Luque","sequence":"additional","affiliation":[{"name":"Electron Microscope Unit, Mark Wainwright Analytical Centre, University of New South Wales Sydney, NSW 2052, Australia"},{"name":"School of Biomedical Sciences, University of New South Wales, Sydney, NSW 2052 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0540-4445","authenticated-orcid":false,"given":"Bernadette","family":"Saunders","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Technology Sydney, Gadigal Country, Sydney, NSW 2007, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4380-7600","authenticated-orcid":false,"given":"Lyndsey E.","family":"Collins-Praino","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Biomedical Science, Adelaide University, Adelaide, SA 5005, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6738-3825","authenticated-orcid":false,"given":"Lars","family":"Ittner","sequence":"additional","affiliation":[{"name":"Dementia Research Centre, Macquarie University, NSW 2109, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0035-7961","authenticated-orcid":false,"given":"Andrew","family":"Care","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Technology Sydney, Gadigal Country, Sydney, NSW 2007, Australia"},{"name":"ARC Centre of Excellence in Synthetic Biology, Macquarie University, NSW 2109, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026060604400552000_2026.06.01.729406v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2868"},{"key":"2026060604400552000_2026.06.01.729406v1.2","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1016\/j.jconrel.2022.12.022","article-title":"Protein-based nanocages for vaccine development","volume":"353","year":"2023","journal-title":"J. Controlled Release"},{"key":"2026060604400552000_2026.06.01.729406v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2016.03.001"},{"key":"2026060604400552000_2026.06.01.729406v1.4","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.pvr.2017.03.004","article-title":"Characterization of a spray-dried candidate HPV L2-VLP vaccine stored for multiple years at room temperature","volume":"3","year":"2017","journal-title":"Papillomavirus Res. Amst. Neth"},{"key":"2026060604400552000_2026.06.01.729406v1.5","doi-asserted-by":"crossref","unstructured":"Yu, Y. B. , Briggs, K. T. , Taraban, M. B. , Brinson, R. G. & Marino, J. P . Grand Challenges in Pharmaceutical Research Series: Ridding the Cold Chain for Biologics. Pharm. Res. 38, 3 (2021).","DOI":"10.1007\/s11095-021-03008-w"},{"key":"2026060604400552000_2026.06.01.729406v1.6","doi-asserted-by":"crossref","unstructured":"Gupta, R. et al. Platforms, advances, and technical challenges in virus-like particles-based vaccines. Front. Immunol. 14, 1123805 (2023).","DOI":"10.3389\/fimmu.2023.1123805"},{"key":"2026060604400552000_2026.06.01.729406v1.7","first-page":"e936523-1","article-title":"Editorial: First Approval of the Protein-Based Adjuvanted Nuvaxovid (NVX-CoV2373) Novavax Vaccine for SARS-CoV-2 Could Increase Vaccine Uptake and Provide Immune Protection from Viral Variants","volume":"28","year":"2022","journal-title":"Med. Sci. Monit. Int. Med. J. Exp. Clin. Res"},{"key":"2026060604400552000_2026.06.01.729406v1.8","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1473"},{"key":"2026060604400552000_2026.06.01.729406v1.9","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.46070"},{"key":"2026060604400552000_2026.06.01.729406v1.10","doi-asserted-by":"crossref","first-page":"8114","DOI":"10.1021\/acsnano.9b03140","article-title":"Iron-Sequestering Nanocompartments as Multiplexed Electron Microscopy Gene Reporters","volume":"13","year":"2019","journal-title":"ACS Nano"},{"key":"2026060604400552000_2026.06.01.729406v1.11","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201488566"},{"key":"2026060604400552000_2026.06.01.729406v1.12","doi-asserted-by":"crossref","first-page":"6209","DOI":"10.1039\/D5MA00386E","article-title":"Engineering encapsulin nanocages for drug delivery","volume":"6","year":"2025","journal-title":"Mater. Adv"},{"key":"2026060604400552000_2026.06.01.729406v1.13","doi-asserted-by":"crossref","unstructured":"Rennie, C. et al. In Vivo Behavior of Systemically Administered Encapsulin Protein Nanocages and Implications for their use in Targeted Drug Delivery. Adv. Ther. 7, 2300360 (2024).","DOI":"10.1002\/adtp.202300360"},{"key":"2026060604400552000_2026.06.01.729406v1.14","doi-asserted-by":"publisher","DOI":"10.1101\/2024.02.24.581861"},{"key":"2026060604400552000_2026.06.01.729406v1.15","doi-asserted-by":"crossref","unstructured":"Corrigan, A. R. et al. Fusion peptide priming reduces immune responses to HIV-1 envelope trimer base. Cell Rep. 35, 108937 (2021).","DOI":"10.1016\/j.celrep.2021.108937"},{"key":"2026060604400552000_2026.06.01.729406v1.16","doi-asserted-by":"crossref","first-page":"4380","DOI":"10.1021\/acsabm.0c00385","article-title":"Facile and Modular Pipeline for Protein-Specific Antibody Customization","volume":"3","year":"2020","journal-title":"ACS Appl. Bio Mater"},{"key":"2026060604400552000_2026.06.01.729406v1.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.07.043"},{"key":"2026060604400552000_2026.06.01.729406v1.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.05.034"},{"key":"2026060604400552000_2026.06.01.729406v1.19","doi-asserted-by":"crossref","unstructured":"Kar, U. et al. Comparative Immunogenicity of Bacterially Expressed Soluble Trimers and Nanoparticle Displayed Influenza Hemagglutinin Stem Immunogens. Front. Immunol. 13, 890622 (2022).","DOI":"10.3389\/fimmu.2022.890622"},{"key":"2026060604400552000_2026.06.01.729406v1.20","doi-asserted-by":"crossref","unstructured":"Gorman, J. et al. Cleavage-intermediate Lassa virus trimer elicits neutralizing responses, identifies neutralizing nanobodies, and reveals an apex-situated site-of-vulnerability. Nat. Commun. 15, 285 (2024).","DOI":"10.1038\/s41467-023-44534-y"},{"key":"2026060604400552000_2026.06.01.729406v1.21","doi-asserted-by":"crossref","unstructured":"Khaleeq, S. et al. Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of Concern. Viruses 15, 346 (2023).","DOI":"10.3390\/v15020346"},{"key":"2026060604400552000_2026.06.01.729406v1.22","first-page":"37","article-title":"Extraordinary Titer and Broad Anti-SARS-CoV-2 Neutralization Induced by Stabilized RBD Nanoparticles from Strain BA.5","volume":"12","year":"2024","journal-title":"Vaccines"},{"key":"2026060604400552000_2026.06.01.729406v1.23","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.3390\/vaccines12091020","article-title":"Molecular Design of Encapsulin Protein Nanoparticles to Display Rotavirus Antigens for Enhancing Immunogenicity","volume":"12","year":"2024","journal-title":"Vaccines"},{"key":"2026060604400552000_2026.06.01.729406v1.24","doi-asserted-by":"crossref","unstructured":"Zhang, Y. et al. Ferritin and Encapsulin Nanoparticles as Effective Vaccine Delivery Systems: Boosting the Immunogenicity of the African Swine Fever Virus C129R Protein. Viruses 17, 556 (2025).","DOI":"10.3390\/v17040556"},{"key":"2026060604400552000_2026.06.01.729406v1.25","doi-asserted-by":"crossref","unstructured":"Zhang, Y. et al. Encapsulin nanoparticle-conjugated p54 protein boosts immune responses against African swine fever virus. Int. J. Biol. Macromol. 311, 143912 (2025).","DOI":"10.1016\/j.ijbiomac.2025.143912"},{"key":"2026060604400552000_2026.06.01.729406v1.26","doi-asserted-by":"crossref","first-page":"4193","DOI":"10.1021\/acsbiomaterials.5c00050","article-title":"Ferritin and Encapsulin Nanoparticles Enhance Immunogenicity of p30 Protein for ASFV Vaccine Development","volume":"11","year":"2025","journal-title":"ACS Biomater. Sci. Eng"},{"key":"2026060604400552000_2026.06.01.729406v1.27","doi-asserted-by":"publisher","DOI":"10.1111\/febs.70561"},{"key":"2026060604400552000_2026.06.01.729406v1.28","doi-asserted-by":"crossref","first-page":"7339","DOI":"10.1021\/acsnano.5b08084","article-title":"Effective Delivery of Antigen\u2013Encapsulin Nanoparticle Fusions to Dendritic Cells Leads to Antigen-Specific Cytotoxic T Cell Activation and Tumor Rejection","volume":"10","year":"2016","journal-title":"ACS Nano"},{"key":"2026060604400552000_2026.06.01.729406v1.29","doi-asserted-by":"crossref","first-page":"1908","DOI":"10.1111\/febs.70340","article-title":"Plant-produced encapsulin displays non-typhoidal Salmonella enterica antigens and assembles into mosaic nanoparticles","volume":"293","year":"2026","journal-title":"FEBS J"},{"key":"2026060604400552000_2026.06.01.729406v1.30","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-018-0289-8"},{"key":"2026060604400552000_2026.06.01.729406v1.31","doi-asserted-by":"crossref","unstructured":"Ross, E. L. , Weinberg, M. S. & Arnold, S. E . Cost-effectiveness of Aducanumab and Donanemab for Early Alzheimer Disease in the US. JAMA Neurol. 79, 478 (2022).","DOI":"10.1001\/jamaneurol.2022.0315"},{"key":"2026060604400552000_2026.06.01.729406v1.32","doi-asserted-by":"crossref","unstructured":"Withington, C. G. & Turner, R. S . Amyloid-Related Imaging Abnormalities With Anti-amyloid Antibodies for the Treatment of Dementia Due to Alzheimer\u2019s Disease. Front. Neurol. 13, 862369 (2022).","DOI":"10.3389\/fneur.2022.862369"},{"key":"2026060604400552000_2026.06.01.729406v1.33","doi-asserted-by":"crossref","first-page":"105940","DOI":"10.1016\/j.ebiom.2025.105940","article-title":"Safety and immunogenicity of two Tau-targeting active immunotherapies, ACI-35.030 and JACI-35.054, in participants with early Alzheimer\u2019s disease: a phase 1b\/2a, multicentre, double-blind, randomised, placebo-controlled study","volume":"120","year":"2025","journal-title":"eBioMedicine"},{"key":"2026060604400552000_2026.06.01.729406v1.34","doi-asserted-by":"publisher","DOI":"10.1159\/000546287"},{"key":"2026060604400552000_2026.06.01.729406v1.35","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25071-y"},{"key":"2026060604400552000_2026.06.01.729406v1.36","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.011882"},{"key":"2026060604400552000_2026.06.01.729406v1.37","doi-asserted-by":"crossref","first-page":"4377","DOI":"10.1039\/D3TB00288H","article-title":"Encapsulin cargo loading: progress and potential","volume":"11","year":"2023","journal-title":"J. Mater. Chem. B"},{"key":"2026060604400552000_2026.06.01.729406v1.38","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1115485109"},{"key":"2026060604400552000_2026.06.01.729406v1.39","doi-asserted-by":"crossref","unstructured":"Brune, K. D. et al. Plug-and-Display: decoration of Virus-Like Particles via isopeptide bonds for modular immunization. Sci. Rep. 6, 19234 (2016).","DOI":"10.1038\/srep19234"},{"key":"2026060604400552000_2026.06.01.729406v1.40","doi-asserted-by":"crossref","unstructured":"Thrane, S. et al. Bacterial superglue enables easy development of efficient virus-like particle based vaccines. J. Nanobiotechnology 14, 30 (2016).","DOI":"10.1186\/s12951-016-0181-1"},{"key":"2026060604400552000_2026.06.01.729406v1.41","doi-asserted-by":"crossref","unstructured":"Aves, K.-L. , Goks\u00f8yr, L. & Sander, A. F . Advantages and Prospects of Tag\/Catcher Mediated Antigen Display on Capsid-Like Particle-Based Vaccines. Viruses 12, 185 (2020).","DOI":"10.3390\/v12020185"},{"key":"2026060604400552000_2026.06.01.729406v1.42","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2005.09.027"},{"key":"2026060604400552000_2026.06.01.729406v1.43","doi-asserted-by":"crossref","unstructured":"Remans, K. et al. Protein purification strategies must consider downstream applications and individual biological characteristics. Microb. Cell Factories 21, 52 (2022).","DOI":"10.1186\/s12934-022-01778-5"},{"key":"2026060604400552000_2026.06.01.729406v1.44","doi-asserted-by":"crossref","first-page":"7142","DOI":"10.1021\/acsomega.4c10285","article-title":"Surface Engineering of the Encapsulin Nanocompartment of Myxococcus xanthus for Cell-Targeted Protein Delivery","volume":"10","year":"2025","journal-title":"ACS Omega"},{"key":"2026060604400552000_2026.06.01.729406v1.45","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.1c01156"},{"key":"2026060604400552000_2026.06.01.729406v1.46","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abl7346"},{"key":"2026060604400552000_2026.06.01.729406v1.47","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-13-1244-1_20"},{"key":"2026060604400552000_2026.06.01.729406v1.48","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026860"},{"key":"2026060604400552000_2026.06.01.729406v1.49","doi-asserted-by":"crossref","unstructured":"Rajamohamedsait, H. , Rasool, S. , Rajamohamedsait, W. , Lin, Y. & Sigurdsson, E. M . Prophylactic Active Tau Immunization Leads to Sustained Reduction in Both Tau and Amyloid-\u03b2 Pathologies in 3xTg Mice. Sci. Rep. 7, 17034 (2017).","DOI":"10.1038\/s41598-017-17313-1"},{"key":"2026060604400552000_2026.06.01.729406v1.50","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2361-07.2007"},{"key":"2026060604400552000_2026.06.01.729406v1.51","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0072301"},{"key":"2026060604400552000_2026.06.01.729406v1.52","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4363-10.2010"},{"key":"2026060604400552000_2026.06.01.729406v1.53","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01576-4"},{"key":"2026060604400552000_2026.06.01.729406v1.54","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200211)32:11<3305::AID-IMMU3305>3.0.CO;2-J"},{"key":"2026060604400552000_2026.06.01.729406v1.55","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.02.103"},{"key":"2026060604400552000_2026.06.01.729406v1.56","doi-asserted-by":"publisher","DOI":"10.1159\/000113700"},{"key":"2026060604400552000_2026.06.01.729406v1.57","doi-asserted-by":"crossref","first-page":"207","DOI":"10.2174\/187152710791012080","article-title":"Abeta DNA Vaccination for Alzheimer\u2019s Disease: Focus on Disease Prevention","volume":"9","year":"2010","journal-title":"CNS Neurol. Disord. Drug Targets"},{"key":"2026060604400552000_2026.06.01.729406v1.58","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.63.10.1459"},{"key":"2026060604400552000_2026.06.01.729406v1.59","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2013.07.006"},{"key":"2026060604400552000_2026.06.01.729406v1.60","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-021-00163-y"},{"key":"2026060604400552000_2026.06.01.729406v1.61","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01576-4"},{"key":"2026060604400552000_2026.06.01.729406v1.62","doi-asserted-by":"crossref","unstructured":"Feng, X. et al. A multi-targeting immunotherapy ameliorates multiple facets of Alzheimer\u2019s disease in 3xTg mice. NPJ Vaccines 9, 153 (2024).","DOI":"10.1038\/s41541-024-00942-9"},{"key":"2026060604400552000_2026.06.01.729406v1.63","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.bbi.2024.08.028","article-title":"A pentavalent peptide vaccine elicits A\u03b2 and tau antibodies with prophylactic activity in an Alzheimer\u2019s disease mouse model","volume":"122","year":"2024","journal-title":"Brain. Behav. Immun"},{"key":"2026060604400552000_2026.06.01.729406v1.64","doi-asserted-by":"crossref","first-page":"6509","DOI":"10.1021\/jacs.9b01523","article-title":"Heterologous Prime-Boost Enhances the Antitumor Immune Response Elicited by Plant-Virus-Based Cancer Vaccine","volume":"141","year":"2019","journal-title":"J. Am. Chem. Soc"},{"key":"2026060604400552000_2026.06.01.729406v1.65","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(02)01357-5"},{"key":"2026060604400552000_2026.06.01.729406v1.66","doi-asserted-by":"crossref","unstructured":"Nazeri, S. , Zakeri, S. , Mehrizi, A. A. , Sardari, S. & Djadid, N. D . Measuring of IgG2c isotype instead of IgG2a in immunized C57BL\/6 mice with Plasmodium vivax TRAP as a subunit vaccine candidate in order to correct interpretation of Th1 versus Th2 immune response. Exp. Parasitol. 216, 107944 (2020).","DOI":"10.1016\/j.exppara.2020.107944"},{"key":"2026060604400552000_2026.06.01.729406v1.67","doi-asserted-by":"publisher","DOI":"10.1111\/nan.12233"},{"key":"2026060604400552000_2026.06.01.729406v1.68","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2016.08.007"},{"key":"2026060604400552000_2026.06.01.729406v1.69","doi-asserted-by":"crossref","unstructured":"van der Hoven, J. et al. Contribution of endogenous antibodies to learning deficits and astrocytosis in human P301S mutant tau transgenic mice. Sci. Rep. 10, 13845 (2020).","DOI":"10.1038\/s41598-020-70845-x"},{"key":"2026060604400552000_2026.06.01.729406v1.70","doi-asserted-by":"crossref","unstructured":"Huang, X. , Wang, X. , Zhang, J. , Xia, N. & Zhao, Q . Escherichia coli-derived virus-like particles in vaccine development. Npj Vaccines 2, 3 (2017).","DOI":"10.1038\/s41541-017-0006-8"},{"key":"2026060604400552000_2026.06.01.729406v1.71","doi-asserted-by":"crossref","unstructured":"Wang, K. et al. Engineering for an HPV 9-valent vaccine candidate using genomic constitutive over-expression and low lipopolysaccharide levels in Escherichia coli cells. Microb. Cell Factories 20, 227 (2021).","DOI":"10.1186\/s12934-021-01719-8"},{"key":"2026060604400552000_2026.06.01.729406v1.72","doi-asserted-by":"crossref","unstructured":"Borcuk, C. et al. Early memory deficits and extensive brain network disorganization in the App\/MAPT double knock-in mouse model of familial Alzheimer\u2019s disease. Aging Brain 2, 100042 (2022).","DOI":"10.1016\/j.nbas.2022.100042"},{"key":"2026060604400552000_2026.06.01.729406v1.73","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abl8809"},{"key":"2026060604400552000_2026.06.01.729406v1.74","doi-asserted-by":"crossref","unstructured":"Plotkin, S. S. & Cashman, N. R . Passive immunotherapies targeting A\u03b2 and tau in Alzheimer\u2019s disease. Neurobiol. Dis. 144, 105010 (2020).","DOI":"10.1016\/j.nbd.2020.105010"},{"key":"2026060604400552000_2026.06.01.729406v1.75","doi-asserted-by":"crossref","unstructured":"Imbimbo, B. P. , Ippati, S. , Watling, M. & Imbimbo, C . Role of monomeric amyloid-\u03b2 in cognitive performance in Alzheimer\u2019s disease: Insights from clinical trials with secretase inhibitors and monoclonal antibodies. Pharmacol. Res. 187, 106631 (2023).","DOI":"10.1016\/j.phrs.2022.106631"},{"key":"2026060604400552000_2026.06.01.729406v1.76","doi-asserted-by":"publisher","DOI":"10.3791\/1350"},{"key":"2026060604400552000_2026.06.01.729406v1.77","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4193"},{"key":"2026060604400552000_2026.06.01.729406v1.78","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2015.11.003"},{"key":"2026060604400552000_2026.06.01.729406v1.79","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2012.09.006"},{"key":"2026060604400552000_2026.06.01.729406v1.80","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4169"},{"key":"2026060604400552000_2026.06.01.729406v1.81","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798318006551"},{"key":"2026060604400552000_2026.06.01.729406v1.82","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20084"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.06.01.729406","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T11:40:34Z","timestamp":1780746034000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.06.01.729406"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,3]]},"references-count":82,"URL":"https:\/\/doi.org\/10.64898\/2026.06.01.729406","relation":{},"subject":[],"published":{"date-parts":[[2026,6,3]]},"subtype":"preprint"}}