{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T06:05:33Z","timestamp":1788588333371,"version":"build-2803163510"},"posted":{"date-parts":[[2026,6,7]]},"group-title":"Immunology","reference-count":112,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T00:00:00Z","timestamp":1780790400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Gates Foundation","award":["INV-027106"],"award-info":[{"award-number":["INV-027106"]}]},{"DOI":"10.13039\/100016394","name":"Harvard Ludwig Center","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100016394","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"NIAID","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,6,18]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                <jats:p>\n                  Tertiary lymphoid structures (TLSs) are sites of immune organization in peripheral tissues that arise from chronic inflammation. They play important roles in infection control and cancer but the mechanisms controlling their formation remain only partly understood. Here, we combine high-plex imaging, serial-section 3D reconstruction, and optimal transport trajectory modeling to reconstruct TLSs in human lungs infected with\n                  <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                  . We find that the extended and irregular shape of TLSs is poorly captured by 2D histopathology or spatial profiling, making assessment of developmental stage (early, primary, or secondary) error prone. In contrast, modeling TLS development in 3D using optimal transport reveals two trajectories that differ in the timing and coordination of follicular dendritic cell association, germinal center consolidation, and position within the tissue. These findings highlight the value of volumetric analysis in understanding immune organization and provide new insights into TLS biology.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Graphical Abstract<\/jats:title>\n                  <jats:fig id=\"ufig1\" position=\"float\" orientation=\"portrait\" fig-type=\"figure\">\n                    <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"725201v2_ufig1\" position=\"float\" orientation=\"portrait\"\/>\n                  <\/jats:fig>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Highlights<\/jats:title>\n                  <jats:list list-type=\"bullet\">\n                    <jats:list-item>\n                      <jats:p>3D reconstruction of TB-infected lung reveals TLSs to be extended, heterogeneous structures that are frequently misclassified in 2D<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>TLS maturation follows continuous trajectories rather than discrete histological stages<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>Optimal transport modeling identifies distinct maturation paths with divergent follicular dynamics<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>Germinal center organization and Tfh-FDC interactions emerge along maturation trajectories<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>TLS maturation states are shaped by spatially localized microenvironmental niches.<\/jats:p>\n                    <\/jats:list-item>\n                  <\/jats:list>\n                <\/jats:sec>","DOI":"10.64898\/2026.06.02.725201","type":"posted-content","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T05:55:14Z","timestamp":1780898114000},"source":"Crossref","is-referenced-by-count":1,"title":["Modeling development of tertiary lymphoid structures in pulmonary tuberculosis by 3D profiling and trajectory analysis"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7378-7957","authenticated-orcid":false,"given":"Emmanuel C.","family":"Ogbonna","sequence":"first","affiliation":[{"name":"Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA 02115, USA"},{"name":"Tufts University School of Medicine, Tufts University, Boston, MA 02111 & Tufts University School of Engineering, Tufts University, Medford, MA 02155, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9999-2403","authenticated-orcid":false,"given":"Soheil R.","family":"Talemi","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4209-7253","authenticated-orcid":false,"given":"Zoltan","family":"Maliga","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2019-1406","authenticated-orcid":false,"given":"Ziyuan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA 02115, USA"},{"name":"Faculty of Arts and Sciences, Harvard University, Cambridge, MA 02138"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1144-5710","authenticated-orcid":false,"given":"Clarence","family":"Yapp","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2686-7898","authenticated-orcid":false,"given":"Shishir M.","family":"Pant","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1740-161X","authenticated-orcid":false,"given":"Yi Daniel","family":"Lu","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0811-637X","authenticated-orcid":false,"given":"Jeremy","family":"Muhlich","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3833-4159","authenticated-orcid":false,"given":"Andr\u00e9anne","family":"Gagn\u00e9","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA 02115, USA"},{"name":"Institut Universitaire de Cardiologie et de Pneumologie de Qu\u00e9bec (Quebec Heart and Lung Institute) Research Center-Laval University and Department of Molecular Biology, Medical Biochemistry, and Pathology, Laval University, Qu\u00e9bec, QC, Canada (current affiliation)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6237-6191","authenticated-orcid":false,"given":"Amanda J.","family":"Martinot","sequence":"additional","affiliation":[{"name":"Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA 01536, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0033-9031","authenticated-orcid":false,"given":"Shannon","family":"Coy","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA 02115, USA"},{"name":"Department of Pathology, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA, 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7528-9668","authenticated-orcid":false,"given":"Sandro","family":"Santagata","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA 02115, USA"},{"name":"Department of Pathology, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA, 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8945-5997","authenticated-orcid":false,"given":"Angela R.","family":"Shih","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Boston, MA 02114, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3432-0902","authenticated-orcid":false,"given":"Isaac H.","family":"Solomon","sequence":"additional","affiliation":[{"name":"Department of Pathology, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA, 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1864-4301","authenticated-orcid":false,"given":"Threnesan","family":"Naidoo","sequence":"additional","affiliation":[{"name":"Africa Health Research Institute, University of KwaZulu-Natal (UKZN), Durban, South Africa"},{"name":"Walter Sisulu University, Mthatha, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2236-1424","authenticated-orcid":false,"given":"Bree B.","family":"Aldridge","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA 02115, USA"},{"name":"Tufts University School of Medicine, Tufts University, Boston, MA 02111 & Tufts University School of Engineering, Tufts University, Medford, MA 02155, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9177-8827","authenticated-orcid":false,"given":"Adrie J.C.","family":"Steyn","sequence":"additional","affiliation":[{"name":"Africa Health Research Institute, University of KwaZulu-Natal (UKZN), Durban, South Africa"},{"name":"Department of Microbiology, University of Alabama, Birmingham, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3364-1838","authenticated-orcid":false,"given":"Peter K.","family":"Sorger","sequence":"additional","affiliation":[{"name":"Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026072300000676000_2026.06.02.725201v2.1","unstructured":"Global Tuberculosis Report 2024 (2024). 1st ed. (World Health Organization)."},{"key":"2026072300000676000_2026.06.02.725201v2.2","unstructured":"WHO TB FACTS SHEET https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/tuberculosis."},{"key":"2026072300000676000_2026.06.02.725201v2.3","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.g5948"},{"key":"2026072300000676000_2026.06.02.725201v2.4","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.69"},{"key":"2026072300000676000_2026.06.02.725201v2.5","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12252"},{"key":"2026072300000676000_2026.06.02.725201v2.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.eclinm.2023.102332"},{"key":"2026072300000676000_2026.06.02.725201v2.7","first-page":"1","article-title":"Recent studies in the epidemiology of tuberculosis, based on the risk of being infected with tubercle bacilli","volume":"19","year":"1976","journal-title":"Adv. Tuberc. Res. Fortschritte Tuberkuloseforschung Progres Explor. Tuberc"},{"key":"2026072300000676000_2026.06.02.725201v2.8","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-3879(82)80013-5"},{"key":"2026072300000676000_2026.06.02.725201v2.9","unstructured":"Tobin, E.H. , and Tristram, D. (2025). Tuberculosis Overview. In StatPearls (StatPearls Publishing)."},{"key":"2026072300000676000_2026.06.02.725201v2.10","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00655-2019"},{"key":"2026072300000676000_2026.06.02.725201v2.11","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwt246"},{"key":"2026072300000676000_2026.06.02.725201v2.12","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-021-01121-x"},{"key":"2026072300000676000_2026.06.02.725201v2.13","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2022.04.004"},{"key":"2026072300000676000_2026.06.02.725201v2.14","doi-asserted-by":"publisher","DOI":"10.1101\/2025.03.10.642376"},{"key":"2026072300000676000_2026.06.02.725201v2.15","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.TBTB2-0016-2016"},{"key":"2026072300000676000_2026.06.02.725201v2.16","doi-asserted-by":"publisher","DOI":"10.1038\/nri3211"},{"key":"2026072300000676000_2026.06.02.725201v2.17","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00411"},{"key":"2026072300000676000_2026.06.02.725201v2.18","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.57.030502.091033"},{"key":"2026072300000676000_2026.06.02.725201v2.19","doi-asserted-by":"publisher","DOI":"10.1172\/JCI65728"},{"key":"2026072300000676000_2026.06.02.725201v2.20","doi-asserted-by":"publisher","DOI":"10.1101\/2022.07.31.502240"},{"key":"2026072300000676000_2026.06.02.725201v2.21","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9533"},{"key":"2026072300000676000_2026.06.02.725201v2.22","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2012.135"},{"key":"2026072300000676000_2026.06.02.725201v2.23","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-023-01476-3"},{"key":"2026072300000676000_2026.06.02.725201v2.24","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1801135"},{"key":"2026072300000676000_2026.06.02.725201v2.25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007142"},{"key":"2026072300000676000_2026.06.02.725201v2.26","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2017.07.073"},{"key":"2026072300000676000_2026.06.02.725201v2.27","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13894"},{"key":"2026072300000676000_2026.06.02.725201v2.28","doi-asserted-by":"publisher","DOI":"10.1111\/imr.70055"},{"key":"2026072300000676000_2026.06.02.725201v2.29","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00113"},{"key":"2026072300000676000_2026.06.02.725201v2.30","doi-asserted-by":"publisher","DOI":"10.1016\/j.thorsurg.2018.12.001"},{"key":"2026072300000676000_2026.06.02.725201v2.31","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-023-00706-z"},{"key":"2026072300000676000_2026.06.02.725201v2.32","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1322035"},{"key":"2026072300000676000_2026.06.02.725201v2.33","doi-asserted-by":"publisher","DOI":"10.1126\/science.abf9419"},{"key":"2026072300000676000_2026.06.02.725201v2.34","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-019-0144-6"},{"key":"2026072300000676000_2026.06.02.725201v2.35","doi-asserted-by":"publisher","DOI":"10.1172\/JCI65728"},{"key":"2026072300000676000_2026.06.02.725201v2.36","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2024.11.010"},{"key":"2026072300000676000_2026.06.02.725201v2.37","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2014.09.006"},{"key":"2026072300000676000_2026.06.02.725201v2.38","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-381300-8.00007-1"},{"key":"2026072300000676000_2026.06.02.725201v2.39","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1147480"},{"key":"2026072300000676000_2026.06.02.725201v2.40","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-022-00619-z"},{"key":"2026072300000676000_2026.06.02.725201v2.41","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2017.1378844"},{"key":"2026072300000676000_2026.06.02.725201v2.42","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2020-002273"},{"key":"2026072300000676000_2026.06.02.725201v2.43","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1096220"},{"key":"2026072300000676000_2026.06.02.725201v2.44","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-17-1987"},{"key":"2026072300000676000_2026.06.02.725201v2.45","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.12.028"},{"key":"2026072300000676000_2026.06.02.725201v2.46","doi-asserted-by":"publisher","DOI":"10.1137\/1.9781611978094"},{"key":"2026072300000676000_2026.06.02.725201v2.47","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.31657"},{"key":"2026072300000676000_2026.06.02.725201v2.48","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-025-02824-x"},{"key":"2026072300000676000_2026.06.02.725201v2.49","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.03.007"},{"key":"2026072300000676000_2026.06.02.725201v2.50","doi-asserted-by":"publisher","DOI":"10.64898\/2026.05.17.725158"},{"key":"2026072300000676000_2026.06.02.725201v2.51","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07359-3"},{"key":"2026072300000676000_2026.06.02.725201v2.52","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-87564-6"},{"key":"2026072300000676000_2026.06.02.725201v2.53","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-66741-5"},{"key":"2026072300000676000_2026.06.02.725201v2.54","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2010.09.025"},{"key":"2026072300000676000_2026.06.02.725201v2.55","unstructured":"ANTsX ANTs: Advanced Normalization Tools."},{"key":"2026072300000676000_2026.06.02.725201v2.56","doi-asserted-by":"publisher","DOI":"10.1109\/TMI.2009.2035616"},{"key":"2026072300000676000_2026.06.02.725201v2.57","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17204-5"},{"key":"2026072300000676000_2026.06.02.725201v2.58","unstructured":"Akenine-M\u00f6ller, T. , and Haines, E. (20). Real-time rendering 2. ed., [Nachdr.]. (AK Peters)."},{"key":"2026072300000676000_2026.06.02.725201v2.59","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-024-02328-0"},{"key":"2026072300000676000_2026.06.02.725201v2.60","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.699336"},{"key":"2026072300000676000_2026.06.02.725201v2.61","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000486"},{"key":"2026072300000676000_2026.06.02.725201v2.62","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2303077120"},{"key":"2026072300000676000_2026.06.02.725201v2.63","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020330404983"},{"key":"2026072300000676000_2026.06.02.725201v2.64","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-08453-2"},{"key":"2026072300000676000_2026.06.02.725201v2.65","doi-asserted-by":"publisher","DOI":"10.1561\/2200000073"},{"key":"2026072300000676000_2026.06.02.725201v2.66","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2024.12.003"},{"key":"2026072300000676000_2026.06.02.725201v2.67","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-024-02186-3"},{"key":"2026072300000676000_2026.06.02.725201v2.68","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-024-01980-6"},{"key":"2026072300000676000_2026.06.02.725201v2.69","doi-asserted-by":"publisher","DOI":"10.1126\/science.abf9419"},{"key":"2026072300000676000_2026.06.02.725201v2.70","doi-asserted-by":"publisher","DOI":"10.1101\/2025.06.23.661064"},{"key":"2026072300000676000_2026.06.02.725201v2.71","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2018.02.010"},{"key":"2026072300000676000_2026.06.02.725201v2.72","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.652631"},{"key":"2026072300000676000_2026.06.02.725201v2.73","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2025.112808"},{"key":"2026072300000676000_2026.06.02.725201v2.74","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.10.032"},{"key":"2026072300000676000_2026.06.02.725201v2.75","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-120419-022408"},{"key":"2026072300000676000_2026.06.02.725201v2.76","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-020-0672-y"},{"key":"2026072300000676000_2026.06.02.725201v2.77","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-20-0949"},{"key":"2026072300000676000_2026.06.02.725201v2.78","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1520112113"},{"key":"2026072300000676000_2026.06.02.725201v2.79","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1789"},{"key":"2026072300000676000_2026.06.02.725201v2.80","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-17135-2"},{"key":"2026072300000676000_2026.06.02.725201v2.81","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2021.12.015"},{"key":"2026072300000676000_2026.06.02.725201v2.82","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-41880-9"},{"key":"2026072300000676000_2026.06.02.725201v2.83","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2017.07.004"},{"key":"2026072300000676000_2026.06.02.725201v2.84","doi-asserted-by":"publisher","DOI":"10.1126\/science.abe5146"},{"key":"2026072300000676000_2026.06.02.725201v2.85","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2025.116250"},{"key":"2026072300000676000_2026.06.02.725201v2.86","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-65421-8"},{"key":"2026072300000676000_2026.06.02.725201v2.87","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-024-01947-5"},{"key":"2026072300000676000_2026.06.02.725201v2.88","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1413067"},{"key":"2026072300000676000_2026.06.02.725201v2.89","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0071-9"},{"key":"2026072300000676000_2026.06.02.725201v2.90","doi-asserted-by":"publisher","DOI":"10.1117\/12.2043282"},{"key":"2026072300000676000_2026.06.02.725201v2.91","doi-asserted-by":"publisher","DOI":"10.1038\/nri3700"},{"key":"2026072300000676000_2026.06.02.725201v2.92","doi-asserted-by":"publisher","DOI":"10.1002\/path.1628"},{"key":"2026072300000676000_2026.06.02.725201v2.93","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4073"},{"key":"2026072300000676000_2026.06.02.725201v2.94","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.202101-0032OC"},{"key":"2026072300000676000_2026.06.02.725201v2.95","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(10)07002-1"},{"key":"2026072300000676000_2026.06.02.725201v2.96","unstructured":"Volumetric Cyclic Immunofluorescence for 3D Spatial Profiling of Immune Structures in Human FFPE Tissue | bioRxiv https:\/\/www.biorxiv.org\/content\/10.64898\/2026.05.17.725158v2."},{"key":"2026072300000676000_2026.06.02.725201v2.97","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1369626"},{"key":"2026072300000676000_2026.06.02.725201v2.98","doi-asserted-by":"publisher","DOI":"10.1101\/2023.11.10.566670"},{"key":"2026072300000676000_2026.06.02.725201v2.99","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6945"},{"key":"2026072300000676000_2026.06.02.725201v2.100","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.34110"},{"key":"2026072300000676000_2026.06.02.725201v2.101","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-021-01308-y"},{"key":"2026072300000676000_2026.06.02.725201v2.102","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14836"},{"key":"2026072300000676000_2026.06.02.725201v2.103","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btac544"},{"key":"2026072300000676000_2026.06.02.725201v2.104","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-04076-3"},{"key":"2026072300000676000_2026.06.02.725201v2.105","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-87564-6"},{"key":"2026072300000676000_2026.06.02.725201v2.106","doi-asserted-by":"publisher","DOI":"10.3389\/fninf.2013.00050"},{"key":"2026072300000676000_2026.06.02.725201v2.107","doi-asserted-by":"publisher","DOI":"10.21105\/joss.06604"},{"key":"2026072300000676000_2026.06.02.725201v2.108","doi-asserted-by":"publisher","DOI":"10.1109\/TIT.1967.1053964"},{"key":"2026072300000676000_2026.06.02.725201v2.109","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0472-9"},{"key":"2026072300000676000_2026.06.02.725201v2.110","doi-asserted-by":"publisher","DOI":"10.1101\/gr.276206.121"},{"key":"2026072300000676000_2026.06.02.725201v2.111","doi-asserted-by":"publisher","DOI":"10.64898\/2026.06.03.725182"},{"key":"2026072300000676000_2026.06.02.725201v2.112","doi-asserted-by":"publisher","DOI":"10.1038\/s43018-023-00576-1"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.06.02.725201","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T07:00:18Z","timestamp":1784790018000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.06.02.725201"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,7]]},"references-count":112,"URL":"https:\/\/doi.org\/10.64898\/2026.06.02.725201","relation":{},"subject":[],"published":{"date-parts":[[2026,6,7]]},"subtype":"preprint"}}