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Here we introduce Rosindol, a novel thioacetal-based fluorogenic probe that overcomes these limitations. Rosindol undergoes an\n                  <jats:italic>umpolung<\/jats:italic>\n                  oxidation in the presence of ROS to generate fluorescence, displaying dose-linear responses to H\n                  <jats:sub>2<\/jats:sub>\n                  O\n                  <jats:sub>2<\/jats:sub>\n                  , O\n                  <jats:sub>2<\/jats:sub>\n                  \u2022\u207b, OH\u2022, and HOCl with minimal background signal. Unlike conventional probes, Rosindol is pH-independent, photostable, water soluble, and agnostic to glucose concentration, esterase expression, and ambient oxygen. Validation in human cells\u2014including PMA-stimulated neutrophils and SOD knockout models\u2014confirms accurate detection of cytosolic and mitochondrial ROS. In pancreatic cancer cells, Rosindol reveals a fourfold increase in mitochondrial O\n                  <jats:sub>2<\/jats:sub>\n                  \u2022\u207b generation capacity via Complex I of the electron transport chain. Glucose stimulation induces twofold higher ROS generation in malignant cells, highlighting a connection between Warburg metabolism and the etiology of oxidative stress in pancreatic cancer. These studies illustrate the utility of Rosindol to provide valuable insight to oxidative stress processes in complex biological environments.\n                <\/jats:p>","DOI":"10.64898\/2026.06.04.730197","type":"posted-content","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T17:55:23Z","timestamp":1781027723000},"source":"Crossref","is-referenced-by-count":0,"title":["Rosindol: A fluorogen for the quantitative measurement of reactive oxygen species in living cells"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5636-4984","authenticated-orcid":false,"given":"Andrew","family":"Monnone","sequence":"first","affiliation":[{"name":"Department of Chemistry, Boston University, Boston MA, USA"},{"name":"Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9888-6259","authenticated-orcid":false,"given":"Molly","family":"Nicknish","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Boston University, Boston MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2462-2018","authenticated-orcid":false,"given":"Juan Jaramillo","family":"Montezco","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Boston University, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9656-3056","authenticated-orcid":false,"given":"Christine","family":"Sanganoo","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Physiology & Biophysics, Chobanian and Avedisian School of Medicine, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nalin","family":"Aggarwal","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Boston University, Boston MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arista","family":"Luong","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Boston University, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5877-6587","authenticated-orcid":false,"given":"Scott","family":"Schaus","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Boston University, Boston MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5453-3668","authenticated-orcid":false,"given":"Mark","family":"Grinstaff","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Boston University, Boston MA, USA"},{"name":"Department of Biomedical Engineering, Boston University, Boston, MA, USA"},{"name":"Boston University Chobanian and Avedisian School of Medicine, Boston, MA, USA"},{"name":"Department of Pharmacology, Physiology & Biophysics, Chobanian and Avedisian School of Medicine, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026061115000584000_2026.06.04.730197v1.1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/2049-3002-2-17","article-title":"Mitochondrial reactive oxygen species and cancer","volume":"2","year":"2014","journal-title":"Cancer Metab"},{"key":"2026061115000584000_2026.06.04.730197v1.2","doi-asserted-by":"publisher","DOI":"10.2174\/1381612043384664"},{"key":"2026061115000584000_2026.06.04.730197v1.3","doi-asserted-by":"publisher","DOI":"10.3109\/10715761003667554"},{"key":"2026061115000584000_2026.06.04.730197v1.4","doi-asserted-by":"publisher","DOI":"10.1111\/cas.15068"},{"key":"2026061115000584000_2026.06.04.730197v1.5","doi-asserted-by":"publisher","DOI":"10.1039\/9781782622208-00001"},{"key":"2026061115000584000_2026.06.04.730197v1.6","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000186787.64500.12"},{"key":"2026061115000584000_2026.06.04.730197v1.7","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/s41420-024-02278-8","article-title":"Free radicals and their impact on health and antioxidant defenses: a review","volume":"11","year":"2025","journal-title":"Cell Death Discov"},{"key":"2026061115000584000_2026.06.04.730197v1.8","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1038\/s42255-022-00591-z","article-title":"Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo","volume":"4","year":"2022","journal-title":"Nat. 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