{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T13:37:57Z","timestamp":1786541877511,"version":"build-2736575974"},"posted":{"date-parts":[[2026,6,15]]},"group-title":"Microbiology","reference-count":40,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the European Union\u2019s Horizon 2020 research and innovation programme under the Marie Sk\u0142odowska-Curie","award":["No. 778069"],"award-info":[{"award-number":["No. 778069"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["N\u00b0 2024.03345.BD"],"award-info":[{"award-number":["N\u00b0 2024.03345.BD"]}]},{"name":"the European Union","award":["N\u00b0 101088806"],"award-info":[{"award-number":["N\u00b0 101088806"]}]},{"name":"the European Union\u2019s Horizon 2020 research and innovation programme","award":["N\u00b0 952374 (BlueBio4Future)"],"award-info":[{"award-number":["N\u00b0 952374 (BlueBio4Future)"]}]},{"DOI":"10.13039\/100000865","name":"the Bill and Melinda Gates Foundation","doi-asserted-by":"crossref","award":["INV-037899"],"award-info":[{"award-number":["INV-037899"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["2023.15022.PEX"],"award-info":[{"award-number":["2023.15022.PEX"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["2023.15022.PEX.F1"],"award-info":[{"award-number":["2023.15022.PEX.F1"]}]},{"name":"the National Center for Complementary and Integrative Health of the NIH","award":["F32AT011475"],"award-info":[{"award-number":["F32AT011475"]}]},{"name":"the National Center for Complementary and Integrative Health of the NIH","award":["R21AI171824"],"award-info":[{"award-number":["R21AI171824"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Boavistamide A (\n                  <jats:bold>1<\/jats:bold>\n                  ), a new alkyne-containing cyclic depsipeptide featuring the rare 3-amino-2-methyl-7-octynoic acid (AMOYA) moiety, was discovered along with two structurally related analogs, boavistamides B and C (\n                  <jats:bold>2<\/jats:bold>\n                  and\n                  <jats:bold>3<\/jats:bold>\n                  ), from a filamentous marine cyanobacterium collected on Boa Vista Island, Cabo Verde. Their isolation was guided by antiplasmodial activity, GNPS MS\/MS molecular networking, LC-MS profiling, and dereplication using the MarinLit database. The planar structures of boavistamides A\u2013C (\n                  <jats:bold>1<\/jats:bold>\n                  \u2013\n                  <jats:bold>3<\/jats:bold>\n                  ) were elucidated through comprehensive HRMS and 1D\/2D NMR analyses, with annotation support from AI-based tools SMART-NMR 2.1 and DeepSAT. The absolute configurations were established using Marfey\u2019s analysis and L-Phe-OMe coupling, complemented by NMR-based conformational studies. Boavistamides A and B exhibited moderate antiplasmodial activity with no mammalian cell cytotoxicity. Microscopic observations and metagenomic binning identified the producer strain as belonging to the genus\n                  <jats:italic>Okeania<\/jats:italic>\n                  (Microcoleaceae). These results expand the chemical diversity of AMOYA-containing cyanobacterial metabolites and highlight the utility of integrated metabolomics and AI-assisted workflows for natural product discovery from environmental samples.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>GRAPHICAL ABSTRACT<\/jats:title>\n                  <jats:fig id=\"ufig1\" position=\"float\" orientation=\"portrait\" fig-type=\"figure\">\n                    <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"732064v1_ufig1\" position=\"float\" orientation=\"portrait\"\/>\n                  <\/jats:fig>\n                <\/jats:sec>","DOI":"10.64898\/2026.06.13.732064","type":"posted-content","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T05:05:23Z","timestamp":1781586323000},"source":"Crossref","is-referenced-by-count":0,"title":["AI-Accelerated Structure Elucidation of Boavistamides A\u2013C, Cyclic Depsipeptides from a Marine Filamentous Cyanobacterium Collected in Cabo Verde"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1728-7901","authenticated-orcid":false,"given":"Marine","family":"Cuau","sequence":"first","affiliation":[{"name":"Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, California 92093, United States"},{"name":"Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, Matosinhos 4450-208, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3588-892X","authenticated-orcid":false,"given":"Nicole E.","family":"Avalon","sequence":"additional","affiliation":[{"name":"Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, California 92093, United States"},{"name":"Department of Pharmaceutical Sciences, School of Pharmacy & Pharmaceutical Sciences, University of California, Irvine, 856 Health Sciences Road, Suite 5400, Irvine, California 92697-3958, United States"},{"name":"Robert A. Mah Molecular Innovation Center, University of California, Irvine, Irvine, California 92697, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3405-2875","authenticated-orcid":false,"given":"Byeol","family":"Ryu","sequence":"additional","affiliation":[{"name":"Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, California 92093, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6513-9909","authenticated-orcid":false,"given":"Evgenia","family":"Glukhov","sequence":"additional","affiliation":[{"name":"Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, California 92093, United 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