{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T07:17:58Z","timestamp":1791530278610,"version":"4.3.4"},"posted":{"date-parts":[[2026,6,15]]},"group-title":"Microbiology","reference-count":43,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"NIAID","doi-asserted-by":"crossref","award":["ZIA AI001254"],"award-info":[{"award-number":["ZIA AI001254"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,6,15]]},"abstract":"<jats:title>Summary<\/jats:title>\n                <jats:p>\n                  Bundibugyo virus (BDBV), a member of the orthoebolaviruses in the\n                  <jats:italic>Filoviridae<\/jats:italic>\n                  family, causes severe hemorrhagic disease with high case-fatality rates. Currently, there are no medical countermeasures approved for human use hampering the response to the large ongoing outbreak in the Democratic Republic of the Congo and Uganda. While vesicular stomatitis virus (VSV)\u2013based vaccines have demonstrated fast-acting prophylactic single-dose efficacy against multiple filoviruses, it has yet to be defined for VSV-BDBV. Here, we evaluated the rapid protection by a single-dose vaccination of VSV\u2013BDBV in nonhuman primates (NHPs). Vaccination elicited rapid innate and early adaptive immune responses and conferred complete protection from clinical disease against BDBV challenge within 3 days. Vaccinated NHPs exhibited minimal clinical signs, limited systemic inflammation, and no infectious virus was isolated from the blood at any time. Protection correlated with neutralizing antibodies and Fc effector functionality of the humoral immune response. These findings establish VSV\u2013BDBV as a fast-acting vaccine candidate suitable for outbreak response and highlight immune mechanisms underlying rapid protection.\n                <\/jats:p>","DOI":"10.64898\/2026.06.14.732188","type":"posted-content","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T05:40:23Z","timestamp":1781588423000},"source":"Crossref","is-referenced-by-count":3,"title":["A Single-Dose Bundibugyo Virus Vaccine Protects Macaques Within 3 Days"],"prefix":"10.64898","author":[{"given":"Kyle L.","family":"O\u2019Donnell","sequence":"first","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jil A.","family":"Haase","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corey W.","family":"Henderson","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. Ryder","family":"Gathright","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paige","family":"Fletcher","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph F.","family":"Rhoderick","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad S.","family":"Clancy","sequence":"additional","affiliation":[{"name":"Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilpi","family":"Jain","sequence":"additional","affiliation":[{"name":"Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9sar","family":"Albari\u00f1o","sequence":"additional","affiliation":[{"name":"Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian J.","family":"Smith","sequence":"additional","affiliation":[{"name":"Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0186-9587","authenticated-orcid":false,"given":"Andrea","family":"Marzi","sequence":"additional","affiliation":[{"name":"Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026061704551158000_2026.06.14.732188v1.1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1901594"},{"key":"2026061704551158000_2026.06.14.732188v1.2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1607.091525"},{"key":"2026061704551158000_2026.06.14.732188v1.3","unstructured":"World Health Organization. Epidemic of Ebola Disease caused by Bundibugyo virus in the Democratic Republic of the Congo and Uganda determined a public health emergency of international concern, <https:\/\/www.who.int\/news\/item\/17-05-2026-epidemic-of-ebola-disease-in-the-democratic-republic-of-the-congo-and-uganda-determined-a-public-health-emergency-of-international-concern> (2026)."},{"key":"2026061704551158000_2026.06.14.732188v1.4","unstructured":"Centers for Disease Control and Prevention. Ebola Outbreak: Current Situation, <https:\/\/www.cdc.gov\/ebola\/situation-summary\/index.html#:~:text=CDC%20is%20responding%20to%20an%20outbreak%20of%20Ebola,to%20the%20American%20public%20and%20travelers%20remains%20low. > (2026)."},{"key":"2026061704551158000_2026.06.14.732188v1.5","unstructured":"CDC. 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