{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"medRxiv"}],"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T08:51:57Z","timestamp":1782118317761,"version":"3.54.5"},"posted":{"date-parts":[[2026,6,18]]},"group-title":"Public and Global Health","reference-count":57,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"National Institute of Child Health and Human Development","award":["R01HD108196"],"award-info":[{"award-number":["R01HD108196"]}]},{"name":"Global One Health Academy at North Carolina State University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,6,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  In low-income countries, ESBL-producing\n                  <jats:italic>Escherichia coli<\/jats:italic>\n                  (ESBL-EC) is frequently detected in humans, animals and household environments, indicating widespread exposure to antimicrobial resistance (AMR). Established risk factors such as antibiotic use do not explain the high community carriage of AMR in all settings; identifying the dominant exposure pathways can inform interventions against AMR. We aimed to investigate (i) animal-human-environment sharing of AMR by assessing associations between the abundance of ESBL-EC in the household environment, domestic animal feces and young children\u2019s stool and (ii) household factors associated with ESBL-EC abundance in these reservoirs. We enrolled 112 households from the CRADLE trial in rural Bangladesh. We enumerated ESBL-EC in drinking water, food, child hand rinses, outdoor soil, indoor floor swabs, chicken and cow feces, and stool from children aged 6 months. We recorded indicators of sanitation, animal ownership\/management, human and animal antibiotic use, and child exposure behaviors using structured questionnaires and spot checks. The highest prevalence of ESBL-EC was in child stool (95.6%) and animal feces (82.3-96.9%), followed by soil (48.2%) and floors (36.6%); &lt; 10% of food, child hands and drinking water harbored ESBL-EC. The abundance of ESBL-EC in child stool was not associated with its abundance in any sampled matrix; the abundance in chicken but not cow feces showed positive correlations with soil, floors, child hands, and drinking water (correlation coefficients: 0.19-0.39, p-values &lt; 0.05). Higher-quality latrines (improved, pour-flush, with slab) were associated with lower ESBL-EC abundance across matrices; unsafe animal management (animals roaming or spending the night inside the home) was associated with higher abundance. Child antibiotic use and exposure behaviors (soil ingestion, time spent on floor) were not associated with ESBL-EC abundance in child stool. We observed high AMR colonization among young children and domestic animals in rural Bangladesh not explained by traditional fecal-oral exposure pathways. Future studies should explore additional pathways and assess whether sanitation and animal management improvements can reduce AMR.\n                <\/jats:p>","DOI":"10.64898\/2026.06.16.26355831","type":"posted-content","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T01:55:18Z","timestamp":1781834118000},"source":"Crossref","is-referenced-by-count":0,"title":["Antimicrobial-resistant\n                  <i>E. coli<\/i>\n                  in human, animal and environmental reservoirs in rural Bangladeshi households with young children"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4305-181X","authenticated-orcid":false,"given":"Sumaiya","family":"Tazin","sequence":"first","affiliation":[{"name":"Department of Forestry and Environmental Resources, North Carolina State University, Raleigh, NC, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Sakib","family":"Hossain","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Health, International Centre for Diarrhoeal Disease Research, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashrin","family":"Haque","sequence":"additional","affiliation":[{"name":"Laboratory of Environmental Health, International Centre for Diarrhoeal Disease Research, Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. 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