{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:46:39Z","timestamp":1782261999834,"version":"3.54.5"},"posted":{"date-parts":[[2026,6,22]]},"group-title":"Pharmacology and Toxicology","reference-count":62,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"crossref","award":["R01ES031285"],"award-info":[{"award-number":["R01ES031285"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"crossref","award":["T32ES007148"],"award-info":[{"award-number":["T32ES007148"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"crossref","award":["P30ES005022"],"award-info":[{"award-number":["P30ES005022"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,6,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Fetal health is heavily dictated by the maternal environment. Inhaling airborne pollutants, like particulate matter, is associated with pregnancy complications and fetal developmental pathologies, including fetal growth restriction (FGR). Because fetal growth is dependent on the placental transfer of nutrients from the maternal circulation, particularly glucose, investigating glucose transport capacity is critical to understanding the development of FGR associated with gestational inhalation of particulate matter. Pregnant Sprague Dawley rats were exposed to titanium dioxide nanoparticles (9.8\u00b11.0 mg\/m\n                  <jats:sup>3<\/jats:sup>\n                  ) as a proxy for ultrafine particulate matter, from gestational day (GD) 5 to GD 19 via whole-body inhalation. Glucose transporters (GLUTs) 1, 3 and 4 were evaluated in term placentas on GD 20 and\n                  <jats:italic>ex vivo<\/jats:italic>\n                  placental perfusion was conducted as a functional assessment of glucose transport. Exposure resulted in a reduction in\n                  <jats:italic>Glut3<\/jats:italic>\n                  mRNA and GLUT1 protein. However, exposed placentas exhibited an adaptation, characterized by increased GLUT4 expression and membrane localization of both GLUT1 and GLUT4. Placental perfusion confirmed these molecular changes, revealing increased glucose flux in exposed placentas compared to control (AUC 95% CI: 77.4 to 127.5 vs 39.1 to 73.6, respectively). Contrary to our hypothesis, exposure to these nanoparticles enhanced glucose transport across the placenta. Here we have demonstrated that inhaling airborne pollutants during pregnancy modulates placental function and nutrient transport mechanisms, which can have direct effects on fetal development. Furthermore, we provide evidence for targeted interventions, aimed at mitigating fetal developmental pathologies.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Highlights<\/jats:title>\n                  <jats:list list-type=\"bullet\">\n                    <jats:list-item>\n                      <jats:p>Gestational inhalation of nanoparticles decreases GLUT1 expression in the placenta.<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>The placenta adapts to gestational nanoparticle inhalation by enhancing GLUT4 expression and GLUT1 and GLUT4 membrane localization.<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>\n                        <jats:italic>Ex vivo<\/jats:italic>\n                        placental perfusion demonstrated increased glucose flux across to the placenta to the fetus following gestational inhalation of nanoparticles.\n                      <\/jats:p>\n                    <\/jats:list-item>\n                  <\/jats:list>\n                <\/jats:sec>","DOI":"10.64898\/2026.06.16.732724","type":"posted-content","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:20:18Z","timestamp":1782174018000},"source":"Crossref","is-referenced-by-count":0,"title":["Inhalation of nanoparticles during pregnancy enhances placental glucose transport in rats"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8969-9190","authenticated-orcid":false,"given":"Talia N.","family":"Seymore","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy"},{"name":"Environmental and Occupational Health Sciences Institute Rutgers University, Piscataway, NJ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Bucknell University, Lewisburg, PA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Louro","sequence":"additional","affiliation":[{"name":"Research Pathology Services, Rutgers University, Piscataway, NJ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6107-8122","authenticated-orcid":false,"given":"Carol","family":"Gardner","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8842-8371","authenticated-orcid":false,"given":"Michael J.","family":"Goedken","sequence":"additional","affiliation":[{"name":"Research Pathology Services, Rutgers University, Piscataway, NJ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3049-4868","authenticated-orcid":false,"given":"Phoebe A.","family":"Stapleton","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy"},{"name":"Environmental and Occupational Health Sciences Institute Rutgers University, Piscataway, NJ"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026062317000954000_2026.06.16.732724v1.1","doi-asserted-by":"crossref","unstructured":"Barker DJP . 2006. 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