{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:49:12Z","timestamp":1791265752904,"version":"4.1.0"},"posted":{"date-parts":[[2026,6,22]]},"group-title":"Genomics","reference-count":55,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014989","name":"Chan Zuckerberg Initiative","doi-asserted-by":"crossref","award":["2019-002425"],"award-info":[{"award-number":["2019-002425"]}],"id":[{"id":"10.13039\/100014989","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100014989","name":"Chan Zuckerberg Initiative","doi-asserted-by":"crossref","award":["2021-239847"],"award-info":[{"award-number":["2021-239847"]}],"id":[{"id":"10.13039\/100014989","id-type":"DOI","asserted-by":"crossref"}]},{"award":["EY022359"],"award-info":[{"award-number":["EY022359"]}],"id":[{"id":"https:\/\/ror.org\/03wkg3b53","id-type":"ROR","asserted-by":"publisher"}]},{"award":["EY028608"],"award-info":[{"award-number":["EY028608"]}],"id":[{"id":"https:\/\/ror.org\/03wkg3b53","id-type":"ROR","asserted-by":"publisher"}]},{"award":["EY025643"],"award-info":[{"award-number":["EY025643"]}],"id":[{"id":"https:\/\/ror.org\/03wkg3b53","id-type":"ROR","asserted-by":"publisher"}]},{"award":["EY032507"],"award-info":[{"award-number":["EY032507"]}],"id":[{"id":"https:\/\/ror.org\/03wkg3b53","id-type":"ROR","asserted-by":"publisher"}]},{"award":["EY034070"],"award-info":[{"award-number":["EY034070"]}],"id":[{"id":"https:\/\/ror.org\/03wkg3b53","id-type":"ROR","asserted-by":"publisher"}]},{"award":["S10OD023469"],"award-info":[{"award-number":["S10OD023469"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,6,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  The trabecular meshwork (TM) and ciliary body (CB) regulate aqueous humor dynamics and intraocular pressure (IOP), and TM\/Schlemm\u2019s canal (SC) dysfunction underlies glaucoma. Here, we present a spatially resolved multi-omics atlas of human TM and CB, integrating snRNA-seq, scRNA-seq, and snATAC-seq from over one million cells and nuclei across 112 donors with Xenium spatial transcriptomics. We identified 9 major cell classes and 21 cell types, revealing heterogeneity, including undercharacterized fibroblast and epithelial subpopulations. Spatial mapping supported TM fibroblast zonation and CB epithelial organization. Regulatory analyses identified cell type\u2013specific programs, including OTX\/PAX networks in CB epithelium and SMAD3\/TGF-\u03b2 signaling in fibroblasts. Integration with glaucoma loci showed enrichment of non-coding variants in regulatory elements associated with POAG and PACG. Age- and ancestry-associated remodeling revealed divergent fibroblast aging with increased\n                  <jats:italic>PIEZO1<\/jats:italic>\n                  , suggesting impaired outflow and elevated IOP. Together, this high-resolution atlas links cellular, regulatory, and genetic variation to anterior segment function and glaucoma susceptibility.\n                <\/jats:p>","DOI":"10.64898\/2026.06.17.732980","type":"posted-content","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T02:15:14Z","timestamp":1782180914000},"source":"Crossref","is-referenced-by-count":2,"title":["An Integrated muti-omics cell atlas of the human trabecular meshwork and ciliary body"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3912-1655","authenticated-orcid":false,"given":"Jinjing","family":"Jian","sequence":"first","affiliation":[{"name":"Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4842-2724","authenticated-orcid":false,"given":"Xuan","family":"Bao","sequence":"additional","affiliation":[{"name":"Brunson Center for Translational Vision Research, Gavin Herbert Eye Institute, Department of Ophthalmology and Visual Sciences, University of California, Irvine, School of Medicine, Irvine, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Zheng","sequence":"additional","affiliation":[{"name":"Brunson Center for Translational Vision Research, Gavin Herbert Eye Institute, Department of Ophthalmology and Visual Sciences, University of California, Irvine, School of Medicine, Irvine, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8975-6776","authenticated-orcid":false,"given":"Jin","family":"Li","sequence":"additional","affiliation":[{"name":"Brunson Center for Translational Vision Research, Gavin Herbert Eye Institute, Department of Ophthalmology and Visual Sciences, University of California, Irvine, School of Medicine, Irvine, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3782-4383","authenticated-orcid":false,"given":"Jianming","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismail","family":"Yaman","sequence":"additional","affiliation":[{"name":"Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Department of Physiology & Biophysics. University of California, Irvine, Irvine, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Chen","sequence":"additional","affiliation":[{"name":"Rory Meyers College of Nursing, New York University, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Tolman","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Vagelos college of Physicians and Surgeons Columbia University Irving Medical Center, New York, NY, USA"},{"name":"Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard H.","family":"Scheuermann","sequence":"additional","affiliation":[{"name":"Division of Intramural Research, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie J.","family":"Zheng","sequence":"additional","affiliation":[{"name":"Stein Eye Institute, Department of Ophthalmology, David Geffen School of Medicine, The Molecular Biology Institute at the University of California, Los Angeles, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl","family":"Sheridan","sequence":"additional","affiliation":[{"name":"Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2870-4504","authenticated-orcid":false,"given":"Yutao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Mayo Clinic, Rochester, MN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqin","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Morsani College of Medicine, University of South Florida, Tampa, FL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Revathi","family":"Balasubramanian","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Vagelos college of Physicians and Surgeons Columbia University Irving Medical Center, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gulab S.","family":"Zode","sequence":"additional","affiliation":[{"name":"Brunson Center for Translational Vision Research, Gavin Herbert Eye Institute, Department of Ophthalmology and Visual Sciences, University of California, Irvine, School of Medicine, Irvine, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4319-0356","authenticated-orcid":false,"given":"Simon WM","family":"John","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Vagelos college of Physicians and Surgeons Columbia University Irving Medical Center, New York, NY, USA"},{"name":"Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Timothy Stout","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Baylor College of Medicine, Houston, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Daniel Stamer","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Duke University School of Medicine, Durham, NC, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumei","family":"Li","sequence":"additional","affiliation":[{"name":"Brunson Center for Translational Vision Research, Gavin Herbert Eye Institute, Department of Ophthalmology and Visual Sciences, University of California, Irvine, School of Medicine, Irvine, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4387-9735","authenticated-orcid":false,"given":"Rui","family":"Chen","sequence":"additional","affiliation":[{"name":"Brunson Center for Translational Vision Research, Gavin Herbert Eye Institute, Department of Ophthalmology and Visual Sciences, University of California, Irvine, School of Medicine, Irvine, CA, USA"},{"name":"Department of Biomedical Engineering, Department of Physiology & Biophysics. 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