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We hypothesized that properties inherent to immune cells, including tissue infiltration and programmed cell recognition, could be harnessed to engineer a cell-based delivery system. We show here that T cells can both produce and transfer editing machinery to target cells. In response to a programmable ligand, engineered T-lymphoid cells can transfer enzymes using complex spatiotemporal logic and deliver cargo in a cell contact-dependent or -independent manner. We demonstrate feasibility of this approach in primary human T cells, establishing a customizable genetic circuit for macromolecular delivery controlled by intercellular interactions.<\/jats:p>","DOI":"10.64898\/2026.06.21.729417","type":"posted-content","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T11:11:14Z","timestamp":1782213074000},"source":"Crossref","is-referenced-by-count":0,"title":["Programming T cells for intercellular genome editing"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6629-6850","authenticated-orcid":false,"given":"Kevin M.","family":"Wasko","sequence":"first","affiliation":[{"name":"Innovative Genomics Institute; University of California, Berkeley; Berkeley CA 94720, USA"},{"name":"Department of Molecular and Cell Biology, University of California, Berkeley; Berkeley, CA 94720, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeleine","family":"Maker","sequence":"additional","affiliation":[{"name":"Innovative Genomics Institute; 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