{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:38:46Z","timestamp":1790915926896,"version":"4.1.0"},"posted":{"date-parts":[[2026,6,26]]},"reference-count":61,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014185","name":"The Searle Scholars Program","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100014185","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Royalty Research Fund"},{"name":"National Institutes of Health Genome Training Grant","award":["T32HG000035"],"award-info":[{"award-number":["T32HG000035"]}]},{"name":"The Bonita and David Brewer Fellowship"},{"name":"The Shurl and Kay Curci Foundation Ph.D. Fellowship"},{"name":"Damon Runyon Postdoctoral Research Fellowship"},{"DOI":"10.13039\/100023581","name":"National Science Foundation Graduate Research Fellowship Program","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100023581","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fluent Biosciences for an in-kind contribution"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,6,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  During embryogenesis, cell types arise in a predictable order because developmental regulators act sequentially. But how evolutionary changes in morphogenesis reshape the signaling environments that activate these regulators remains unknown. Across vertebrates, primitive myeloid cells emerge from bone morphogenetic protein (BMP)-patterned ventral mesoderm through a conserved regulatory program. Here we show that in the annual killifish,\n                  <jats:italic>Nothobranchius furzeri<\/jats:italic>\n                  , a vertebrate with highly derived embryogenesis, neutrophils emerge prior to gastrulation, before ventral mesoderm has formed. Vascular progenitors arise later from ventral mesoderm, whereas myeloid progenitors are largely absent from this tissue. BMP inhibition abolishes pre-gastrula neutrophil specification, while disruption of Nodal-dependent mesendoderm formation does not. These findings reveal that conserved cell type programs can be redeployed within an altered embryonic architecture.\n                <\/jats:p>","DOI":"10.64898\/2026.06.25.734555","type":"posted-content","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T14:20:23Z","timestamp":1782483623000},"source":"Crossref","is-referenced-by-count":1,"title":["Early immune cell development precedes gastrulation in annual killifish"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5551-3378","authenticated-orcid":false,"given":"Sydney M.","family":"Sattler","sequence":"first","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, WA 98105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6832-6152","authenticated-orcid":false,"given":"Nicholas C.","family":"Lammers","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, WA 98105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3085-7451","authenticated-orcid":false,"given":"Sebastian V.","family":"Hriscu","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, WA 98105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2775-733X","authenticated-orcid":false,"given":"Clare L.T.","family":"Booth","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, WA 98105, USA"},{"name":"Graduate Program in Molecular & Cellular Biology, University of Washington, Seattle, WA 98105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8105-4347","authenticated-orcid":false,"given":"Cole","family":"Trapnell","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, WA 98105, USA"},{"name":"Seattle Hub for Synthetic Biology, Seattle, WA 98109, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8264-0807","authenticated-orcid":false,"given":"Philip B.","family":"Abitua","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, WA 98105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026063003351050000_2026.06.25.734555v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.02.017"},{"key":"2026063003351050000_2026.06.25.734555v1.2","doi-asserted-by":"publisher","DOI":"10.1111\/BRV.12183"},{"key":"2026063003351050000_2026.06.25.734555v1.3","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1401820203"},{"key":"2026063003351050000_2026.06.25.734555v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2018.07.015"},{"key":"2026063003351050000_2026.06.25.734555v1.5","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1002\/dvdy.24496","article-title":"Gastrulation in an annual killifish: Molecular and cellular events during germ layer formation in Austrolebias","volume":"246","year":"2017","journal-title":"Dev. 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